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CLC number: R285.5

On-line Access: 2017-09-05

Received: 2016-04-28

Revision Accepted: 2016-09-17

Crosschecked: 2017-08-17

Cited: 0

Clicked: 2261

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Zhong-ping Deng

http://orcid.org/0000-0002-5608-8641

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.9 P.757-769

http://doi.org/10.1631/jzus.B1600158


Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish


Author(s):  Hong-cui Liu, Xiao-yu Zhu, Jiang-hua Chen, Sheng-ya Guo, Chun-qi Li, Zhong-ping Deng

Affiliation(s):  Center for Drug Safety Evaluation and Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; more

Corresponding email(s):   jackli@zhunter.com, dzp@shutcm.edu.cn

Key Words:  Sophorae tonkinensis Gagnep, Active fraction, Hepatotoxicity, Cardiovascular toxicity, Zebrafish


Hong-cui Liu, Xiao-yu Zhu, Jiang-hua Chen, Sheng-ya Guo, Chun-qi Li, Zhong-ping Deng. Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish[J]. Journal of Zhejiang University Science B, 2017, 18(9): 757-769.

@article{title="Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish",
author="Hong-cui Liu, Xiao-yu Zhu, Jiang-hua Chen, Sheng-ya Guo, Chun-qi Li, Zhong-ping Deng",
journal="Journal of Zhejiang University Science B",
volume="18",
number="9",
pages="757-769",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600158"
}

%0 Journal Article
%T Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish
%A Hong-cui Liu
%A Xiao-yu Zhu
%A Jiang-hua Chen
%A Sheng-ya Guo
%A Chun-qi Li
%A Zhong-ping Deng
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 9
%P 757-769
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600158

TY - JOUR
T1 - Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish
A1 - Hong-cui Liu
A1 - Xiao-yu Zhu
A1 - Jiang-hua Chen
A1 - Sheng-ya Guo
A1 - Chun-qi Li
A1 - Zhong-ping Deng
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 9
SP - 757
EP - 769
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600158


Abstract: 
Radix Sophorae tonkinensis (RST) is a widely used herb in Traditional Chinese Medicine (TCM) for treating infectious and inflammatory diseases. However, the toxicity data for RST are limited. The aim of this work is to assess and compare the toxicity of the whole RST extract and its five active fractions using the zebrafish model. Five active fractions of RST were prepared using five different types of solvents, which included dealkalized water, ethanol, n-butyl ethanol, dichloromethane, and diethyl ether. The chemical profiles of the active fractions were determined by high-performance liquid chromatography (HPLC), and the toxicity observed in the zebrafish model was confirmed using mouse models. In the zebrafish model, cardiovascular toxicity was observed for the fraction extracted using diethyl ether, and hepatotoxicity was observed for the whole RST extract and the fractions extracted using water and ethanol, whereas both cardiovascular and hepatic toxicities were observed for the fractions extracted using n-butyl ethanol and dichloromethane. The hepatotoxicity of the fractions extracted using n-butyl ethanol and dichloromethane was also observed in mice. Our findings provide the toxicity data for RST and its five active fractions through modeling in a zebrafish, and indicate that the different fractions may each have a different toxicity, which is helpful for the optimal use of RST in clinical practice.

使用斑马鱼模型比较山豆根不同有效部位的毒性

目的:利用斑马鱼模型评价和比较山豆根不同提取方法提取的有效部位的体内毒性。
创新点:首次在斑马鱼模型中证明山豆根提取方法不同,有效部位的毒性有明显差异。研究结果有助于指导山豆根的新药开发与临床应用。
方法:用五种不同的溶剂(去碱水、乙醇、正丁基乙醇、二氯甲烷和乙醚)提取山豆根,然后通过高效液相色谱法(HPLC)检测有效部位,将AB品系斑马鱼分为对照组(养鱼水处理)和实验组(山豆根提取物)。实验组根据采用的提取溶剂不同,分为以下六组:去碱水提取组、乙醇沉提取组、正丁基乙醇提取组、二氯甲烷提取组和乙醚提取组以及山豆根总组分组(对照),观察各种山豆根提取物对斑马鱼的急性毒性与毒性靶器官。
结论:山豆根乙醚提取组诱发斑马鱼心血管毒性(图1);山豆根去碱水提取组、乙醇沉提取组以及山豆根总组分组诱发斑马鱼肝脏毒性(图3和图4);而山豆根正丁基乙醇提取组和二氯甲烷提取组诱发斑马鱼心血管毒性(图1和图2)和肝脏毒性(图3和图4)。

关键词:山豆根;有效部位;肝脏毒性;心血管毒性;斑马鱼

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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