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CLC number: R285.5

On-line Access: 2017-09-05

Received: 2016-04-28

Revision Accepted: 2016-09-17

Crosschecked: 2017-08-17

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Zhong-ping Deng


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.9 P.757-769


Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish

Author(s):  Hong-cui Liu, Xiao-yu Zhu, Jiang-hua Chen, Sheng-ya Guo, Chun-qi Li, Zhong-ping Deng

Affiliation(s):  Center for Drug Safety Evaluation and Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; more

Corresponding email(s):   jackli@zhunter.com, dzp@shutcm.edu.cn

Key Words:  Sophorae tonkinensis Gagnep, Active fraction, Hepatotoxicity, Cardiovascular toxicity, Zebrafish

Hong-cui Liu, Xiao-yu Zhu, Jiang-hua Chen, Sheng-ya Guo, Chun-qi Li, Zhong-ping Deng. Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish[J]. Journal of Zhejiang University Science B, 2017, 18(9): 757-769.

@article{title="Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish",
author="Hong-cui Liu, Xiao-yu Zhu, Jiang-hua Chen, Sheng-ya Guo, Chun-qi Li, Zhong-ping Deng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish
%A Hong-cui Liu
%A Xiao-yu Zhu
%A Jiang-hua Chen
%A Sheng-ya Guo
%A Chun-qi Li
%A Zhong-ping Deng
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 9
%P 757-769
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600158

T1 - Toxicity comparison of different active fractions extracted from radix Sophorae tonkinensis in zebrafish
A1 - Hong-cui Liu
A1 - Xiao-yu Zhu
A1 - Jiang-hua Chen
A1 - Sheng-ya Guo
A1 - Chun-qi Li
A1 - Zhong-ping Deng
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 9
SP - 757
EP - 769
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600158

Radix Sophorae tonkinensis (RST) is a widely used herb in Traditional Chinese Medicine (TCM) for treating infectious and inflammatory diseases. However, the toxicity data for RST are limited. The aim of this work is to assess and compare the toxicity of the whole RST extract and its five active fractions using the zebrafish model. Five active fractions of RST were prepared using five different types of solvents, which included dealkalized water, ethanol, n-butyl ethanol, dichloromethane, and diethyl ether. The chemical profiles of the active fractions were determined by high-performance liquid chromatography (HPLC), and the toxicity observed in the zebrafish model was confirmed using mouse models. In the zebrafish model, cardiovascular toxicity was observed for the fraction extracted using diethyl ether, and hepatotoxicity was observed for the whole RST extract and the fractions extracted using water and ethanol, whereas both cardiovascular and hepatic toxicities were observed for the fractions extracted using n-butyl ethanol and dichloromethane. The hepatotoxicity of the fractions extracted using n-butyl ethanol and dichloromethane was also observed in mice. Our findings provide the toxicity data for RST and its five active fractions through modeling in a zebrafish, and indicate that the different fractions may each have a different toxicity, which is helpful for the optimal use of RST in clinical practice.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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