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CLC number: S626

On-line Access: 2017-07-05

Received: 2016-06-23

Revision Accepted: 2016-11-16

Crosschecked: 2017-06-16

Cited: 0

Clicked: 2020

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Tao Lu

http://orcid.org/0000-0003-0098-4356

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.7 P.635-648

10.1631/jzus.B1600286


Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato


Author(s):  Tao Lu, Jie-wei Shi, Zhou-ping Sun, Ming-fang Qi, Yu-feng Liu, Tian-lai Li

Affiliation(s):  College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China; more

Corresponding email(s):   lyf-3939@163.com, tianlaili@126.com

Key Words:  Photoinhibition, Photoprotection, Thylakoid membrane, Tomato


Tao Lu, Jie-wei Shi, Zhou-ping Sun, Ming-fang Qi, Yu-feng Liu, Tian-lai Li. Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato[J]. Journal of Zhejiang University Science B, 2017, 18(7): 635-648.

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journal="Journal of Zhejiang University Science B",
volume="18",
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doi="10.1631/jzus.B1600286"
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%T Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato
%A Tao Lu
%A Jie-wei Shi
%A Zhou-ping Sun
%A Ming-fang Qi
%A Yu-feng Liu
%A Tian-lai Li
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600286

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T1 - Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato
A1 - Tao Lu
A1 - Jie-wei Shi
A1 - Zhou-ping Sun
A1 - Ming-fang Qi
A1 - Yu-feng Liu
A1 - Tian-lai Li
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1600286


Abstract: 
Objective: To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress. Methods: Six-leaf-stage tomato seedlings (“Liaoyuanduoli”, n=160) were divided into four parts: Part 1, served as control under 25 °C, 500 µmol/(m2·s); Part 2, spayed with distilled water (H2O) under 35 °C, 1000 µmol/(m2·s) (HH); Part 3, spayed with 100 µmol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 µmol/L methyl viologen (MV, LEF inhibitor) under HH. Energy conversion, photosystem I (PSI), and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d. Results: HH decreased photochemical reaction dissipation (P) and the maximal photochemical efficiency of PSII (Fv/Fm), and increased the excitation energy distribution coefficient of PSII (β); DCMU and MV aggravated the partition imbalance of the excitation energy (γ) and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI (ETRI and YI) significantly decreased whereas acceptor and donor side limitation of PSI (YNA and YND) increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy YNPQ and YNO, respectively. Membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions. Conclusions: The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity.

线性电子传递和环式电子传递对缓解番茄亚高温强光胁迫的响应

目的:探讨特定高温和强光逆境下番茄叶片中的环式电子传递(CEF)和线性电子传递(LEF)的光保护机制。
创新点:通过引入电子抑制剂的方法系统分析了CEF和LEF对亚高温强光胁迫的响应。
方法:将品种为"辽园多丽"的番茄幼苗(n=160)平均分成四组(表1):组1,于常温常光照25 °C,500 µmol/(m2·s)条件下培养并作为对照;组2,叶片喷施蒸馏水并在亚高温强光35 °C,1000 µmol/(m2·s)(HH)条件下培养;组3,HH条件下叶片喷施100 µmol/L敌草隆(DCMU,CEF抑制剂);组4,HH条件下叶片喷施60 µmol/L甲基紫精(MV,LEF抑制剂)。在处理5 d及恢复10 d期间,分别测定番茄幼苗叶片的光能吸收、激发能分配、光系统活性、类囊体膜完整性和ATP酶活性等指标。
结论:CEF和LEF通过一定程度上改善叶片光能吸收及激发能分配,并且维持类囊体膜较高完整性和ATP酶活性,从而维持光系统活性并减少光抑制和光破坏程度。

关键词:光抑制;光保护;类囊体膜;番茄

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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