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Received: 2016-06-23

Revision Accepted: 2016-11-16

Crosschecked: 2017-06-16

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Tao Lu


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.7 P.635-648


Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato

Author(s):  Tao Lu, Jie-wei Shi, Zhou-ping Sun, Ming-fang Qi, Yu-feng Liu, Tian-lai Li

Affiliation(s):  College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China; more

Corresponding email(s):   lyf-3939@163.com, tianlaili@126.com

Key Words:  Photoinhibition, Photoprotection, Thylakoid membrane, Tomato

Tao Lu, Jie-wei Shi, Zhou-ping Sun, Ming-fang Qi, Yu-feng Liu, Tian-lai Li. Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato[J]. Journal of Zhejiang University Science B, 2017, 18(7): 635-648.

@article{title="Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato",
author="Tao Lu, Jie-wei Shi, Zhou-ping Sun, Ming-fang Qi, Yu-feng Liu, Tian-lai Li",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato
%A Tao Lu
%A Jie-wei Shi
%A Zhou-ping Sun
%A Ming-fang Qi
%A Yu-feng Liu
%A Tian-lai Li
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 7
%P 635-648
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600286

T1 - Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato
A1 - Tao Lu
A1 - Jie-wei Shi
A1 - Zhou-ping Sun
A1 - Ming-fang Qi
A1 - Yu-feng Liu
A1 - Tian-lai Li
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 7
SP - 635
EP - 648
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600286

Objective: To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress. Methods: Six-leaf-stage tomato seedlings (“Liaoyuanduoli”, n=160) were divided into four parts: Part 1, served as control under 25 °C, 500 µmol/(m2·s); Part 2, spayed with distilled water (H2O) under 35 °C, 1000 µmol/(m2·s) (HH); Part 3, spayed with 100 µmol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 µmol/L methyl viologen (MV, LEF inhibitor) under HH. Energy conversion, photosystem I (PSI), and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d. Results: HH decreased photochemical reaction dissipation (P) and the maximal photochemical efficiency of PSII (Fv/Fm), and increased the excitation energy distribution coefficient of PSII (β); DCMU and MV aggravated the partition imbalance of the excitation energy (γ) and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI (ETRI and YI) significantly decreased whereas acceptor and donor side limitation of PSI (YNA and YND) increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy YNPQ and YNO, respectively. Membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions. Conclusions: The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity.


方法:将品种为"辽园多丽"的番茄幼苗(n=160)平均分成四组(表1):组1,于常温常光照25 °C,500 µmol/(m2·s)条件下培养并作为对照;组2,叶片喷施蒸馏水并在亚高温强光35 °C,1000 µmol/(m2·s)(HH)条件下培养;组3,HH条件下叶片喷施100 µmol/L敌草隆(DCMU,CEF抑制剂);组4,HH条件下叶片喷施60 µmol/L甲基紫精(MV,LEF抑制剂)。在处理5 d及恢复10 d期间,分别测定番茄幼苗叶片的光能吸收、激发能分配、光系统活性、类囊体膜完整性和ATP酶活性等指标。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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