Full Text:   <657>

Summary:  <172>

CLC number: R378.99

On-line Access: 2018-05-05

Received: 2017-07-24

Revision Accepted: 2017-09-06

Crosschecked: 2018-04-18

Cited: 0

Clicked: 1521

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Min-tao Zhong

https://orcid.org/0000-0002-2190-1153

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.5 P.364-371

10.1631/jzus.B1700356


Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa


Author(s):  Wen-dong Wang, Nan-nan Zhang, Warren Chanda, Min Liu, Syed Riaz ud Din, Yun-peng Diao, Lei Liu, Jing Cao, Xiao-li Wang, Xing-yun Li, An-hong Ning, Min Huang, Min-tao Zhong

Affiliation(s):  Department of Medical Microbiology, Dalian Medical University, Dalian 116044, China; more

Corresponding email(s):   huangminchao@163.com, dyzhongmt@163.com

Key Words:  Antibacterial, Lipid extract, Mantidis ootheca, Pseudomonas aeruginosa


Wen-dong Wang, Nan-nan Zhang, Warren Chanda, Min Liu, Syed Riaz ud Din, Yun-peng Diao, Lei Liu, Jing Cao, Xiao-li Wang, Xing-yun Li, An-hong Ning, Min Huang, Min-tao Zhong. Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa[J]. Journal of Zhejiang University Science B, 2018, 19(5): 364-371.

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author="Wen-dong Wang, Nan-nan Zhang, Warren Chanda, Min Liu, Syed Riaz ud Din, Yun-peng Diao, Lei Liu, Jing Cao, Xiao-li Wang, Xing-yun Li, An-hong Ning, Min Huang, Min-tao Zhong",
journal="Journal of Zhejiang University Science B",
volume="19",
number="5",
pages="364-371",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700356"
}

%0 Journal Article
%T Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa
%A Wen-dong Wang
%A Nan-nan Zhang
%A Warren Chanda
%A Min Liu
%A Syed Riaz ud Din
%A Yun-peng Diao
%A Lei Liu
%A Jing Cao
%A Xiao-li Wang
%A Xing-yun Li
%A An-hong Ning
%A Min Huang
%A Min-tao Zhong
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 5
%P 364-371
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700356

TY - JOUR
T1 - Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa
A1 - Wen-dong Wang
A1 - Nan-nan Zhang
A1 - Warren Chanda
A1 - Min Liu
A1 - Syed Riaz ud Din
A1 - Yun-peng Diao
A1 - Lei Liu
A1 - Jing Cao
A1 - Xiao-li Wang
A1 - Xing-yun Li
A1 - An-hong Ning
A1 - Min Huang
A1 - Min-tao Zhong
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 5
SP - 364
EP - 371
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700356


Abstract: 
The aim of this study is to assess the antibacterial and anti-biofilm properties of the lipid extract from Mantidis ootheca against the gentamycin resistant Pseudomonas aeruginosa. The chemical composition of the lipid extract and its relative proportion were determined using the technique of gas chromatography coupled with mass spectrometry (GC-MS). antibacterial susceptibility tests were performed using a disc diffusion assay and the minimum inhibition concentration (MIC) was determined by way of the agar dilution method. The anti-biofilm test was carried out with crystal violet staining and scanning electron microscopy (SEM). There were 16 compounds detected, and the most abundant components were sesquiterpenoids, monoterpenes, and trace aromatic compounds. The MIC for P. aeruginosa was 4 mg/ml and the eradication effect on preformed biofilms was established and compared with a ciprofloxacin control. The results of our study indicated that a lipid extract from M. ootheca could be used as a topical and antibacterial agent with anti-biofilm activity in the future.

桑螵蛸脂类提取物对铜绿假单胞菌的抗菌活性和抗生物膜作用

目的:探究桑螵蛸脂类提取物的成分及对铜绿假单胞菌的抗菌和抗生物膜作用.
创新点:中国传统中药桑螵蛸一直广泛应用于肾病的治疗,在抗菌领域未见报道,本实验首次证明桑螵蛸脂类提取物对铜绿假单胞菌有明显的抗菌和抗生物膜作用.
方法:采用气相色谱-质谱联用技术,测定桑螵蛸脂质提取物的化学成分及其相对比例.采用纸片扩散法和琼脂平板稀释法观察桑螵蛸脂类提取物对铜绿假单胞菌的抑菌效应并测定最小抑制浓度(MIC).采用结晶紫染色法和扫描电镜(SEM)进行抑制生物被膜的试验.
结论:桑螵蛸脂类提取物中含有16种化合物,最丰富的成分分别是倍半萜类化合物、单萜和微量芳香族化合物.桑螵蛸脂类提取物对铜绿假单胞菌的MIC为4 mg/ml,对铜绿假单胞菌生物被膜的抑制作用明显.

关键词:抗菌药;脂类提取物;桑螵蛸;铜绿假单胞菌

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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