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On-line Access: 2022-04-11

Received: 2021-08-29

Revision Accepted: 2021-12-08

Crosschecked: 2022-04-19

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Xing DU


Xufeng FU


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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.4 P.286-299


A novel anticancer property of Lycium barbarum polysaccharide in triggering ferroptosis of breast cancer cells

Author(s):  Xing DU, Jingjing ZHANG, Ling LIU, Bo XU, Hang HAN, Wenjie DAI, Xiuying PEI, Xufeng FU, Shaozhang HOU

Affiliation(s):  Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China; more

Corresponding email(s):   fuxufeng100@163.com, houshzh@nxmu.edu.cn

Key Words:  Lycium barbarum polysaccharide, Ferroptosis, Breast cancer cells, xCT, Glutathione peroxidase 4 (GPX4)

Xing DU, Jingjing ZHANG, Ling LIU, Bo XU, Hang HAN, Wenjie DAI, Xiuying PEI, Xufeng FU, Shaozhang HOU. A novel anticancer property of Lycium barbarum polysaccharide in triggering ferroptosis of breast cancer cells[J]. Journal of Zhejiang University Science B, 2022, 23(4): 286-299.

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author="Xing DU, Jingjing ZHANG, Ling LIU, Bo XU, Hang HAN, Wenjie DAI, Xiuying PEI, Xufeng FU, Shaozhang HOU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T A novel anticancer property of Lycium barbarum polysaccharide in triggering ferroptosis of breast cancer cells
%A Xing DU
%A Jingjing ZHANG
%A Ling LIU
%A Bo XU
%A Hang HAN
%A Wenjie DAI
%A Xiuying PEI
%A Xufeng FU
%A Shaozhang HOU
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 4
%P 286-299
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100748

T1 - A novel anticancer property of Lycium barbarum polysaccharide in triggering ferroptosis of breast cancer cells
A1 - Xing DU
A1 - Jingjing ZHANG
A1 - Ling LIU
A1 - Bo XU
A1 - Hang HAN
A1 - Wenjie DAI
A1 - Xiuying PEI
A1 - Xufeng FU
A1 - Shaozhang HOU
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 4
SP - 286
EP - 299
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100748

Breast cancer is one of the most malignant tumors and is associated with high mortality rates among women. Lycium barbarum polysaccharide (LBP) is an extract from the fruits of the traditional Chinese herb, L. barbarum. LBP is a promising anticancer drug, due to its high activity and low toxicity. Although it has anticancer properties, its mechanisms of action have not been fully established. ferroptosis, which is a novel anticancer strategy, is a cell death mechanism that relies on iron-dependent lipid reactive oxygen species (ROS) accumulation. In this study, human breast cancer cells (Michigan Cancer Foundation-7 (MCF-7) and MD Anderson-Metastatic Breast-231 (MDA-MB-231)) were treated with LBP. LBP inhibited their viability and proliferation in association with high levels of ferroptosis. Therefore, we aimed to ascertain whether LBP reduced cell viability through ferroptosis. We found that the structure and function of mitochondria, lipid peroxidation, and expression of solute carrier family 7 member 11 (SLC7A11, also known as xCT, the light-chain subunit of cystine/glutamate antiporter system Xc-) and glutathione peroxidase 4 (GPX4) were altered by LBP. Moreover, the ferroptosis inhibitor, Ferrostatin-1 (Fer-1), rescued LBP-induced ferroptosis-associated events including reduced cell viability and glutathione (GSH) production, accumulation of intracellular free divalent iron ions and malondialdehyde (MDA), and down-regulation of the expression of xCT and GPX4. Erastin (xCT inhibitor) and RSL3 (GPX4 inhibitor) inhibited the expression of xCT and GPX4, respectively, which was lower after the co-treatment of LBP with Erastin and RSL3. These results suggest that LBP effectively prevents breast cancer cell proliferation and promotes ferroptosis via the xCT/GPX4 pathway. Therefore, LBP exhibits novel anticancer properties by triggering ferroptosis, and may be a potential therapeutic option for breast cancer.


目的:乳腺癌是女性高发的恶性肿瘤之一。枸杞多糖(Lycium barbarumpolysaccharide,LBP)作为我国传统名贵中药材枸杞子的主要活性成分,因其高活性和低毒性逐渐成为抗癌药物的新选择。已有研究表明LBP对多种肿瘤细胞的生长具有抑制作用,但机制尚不明确。铁死亡是一种新的细胞死亡方式,主要依赖铁的脂质过氧化,引发细胞死亡,而LBP与铁死亡的关系尚未见明确报道。因此,本研究旨在探索LBP是否通过诱导铁死亡发挥抗癌作用。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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