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On-line Access: 2024-08-27

Received: 2023-10-17

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Crosschecked: 2022-11-16

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 ORCID:

Lee JIA

https://orcid.org/0000-0001-6839-5545

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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.11 P.943-956

http://doi.org/10.1631/jzus.B2200232


Combination of Se-methylselenocysteine, D-α-tocopheryl succinate, β-carotene, and L-lysine can prevent cancer metastases using as an adjuvant therapy


Author(s):  Yunlong CHENG, Shu LIAN, Shuhui LI, Yusheng LU, Jie WANG, Xiaoxiao DENG, Shengyi ZHAI, Lee JIA

Affiliation(s):  Fujian-Taiwan-Hongkong-Macao Science and Technology Cooperation Base of Intelligent Pharmaceutics, College of Materials and Chemical Engineering, Minjiang University, Fuzhou 350108, China; more

Corresponding email(s):   cmapcjia1234@163.com, jiali@fzu.edu.cn

Key Words:  Cancer prevention, Cancer metastases, Selenium (Se), Combination of drugs, Nutrition


Yunlong CHENG, Shu LIAN, Shuhui LI, Yusheng LU, Jie WANG, Xiaoxiao DENG, Shengyi ZHAI, Lee JIA. Combination of Se-methylselenocysteine, D-α-tocopheryl succinate, β-carotene, and L-lysine can prevent cancer metastases using as an adjuvant therapy[J]. Journal of Zhejiang University Science B, 2022, 23(11): 943-956.

@article{title="Combination of Se-methylselenocysteine, D-α-tocopheryl succinate, β-carotene, and L-lysine can prevent cancer metastases using as an adjuvant therapy",
author="Yunlong CHENG, Shu LIAN, Shuhui LI, Yusheng LU, Jie WANG, Xiaoxiao DENG, Shengyi ZHAI, Lee JIA",
journal="Journal of Zhejiang University Science B",
volume="23",
number="11",
pages="943-956",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200232"
}

%0 Journal Article
%T Combination of Se-methylselenocysteine, D-α-tocopheryl succinate, β-carotene, and L-lysine can prevent cancer metastases using as an adjuvant therapy
%A Yunlong CHENG
%A Shu LIAN
%A Shuhui LI
%A Yusheng LU
%A Jie WANG
%A Xiaoxiao DENG
%A Shengyi ZHAI
%A Lee JIA
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 11
%P 943-956
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200232

TY - JOUR
T1 - Combination of Se-methylselenocysteine, D-α-tocopheryl succinate, β-carotene, and L-lysine can prevent cancer metastases using as an adjuvant therapy
A1 - Yunlong CHENG
A1 - Shu LIAN
A1 - Shuhui LI
A1 - Yusheng LU
A1 - Jie WANG
A1 - Xiaoxiao DENG
A1 - Shengyi ZHAI
A1 - Lee JIA
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 11
SP - 943
EP - 956
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200232


Abstract: 
ObjectivePrimary tumor treatment through surgical resection and adjuvant therapy has been extensively studied, but there is a lack of effective strategies and drugs for the treatment of tumor metastases. Here, we describe a functional product based on a combination of compounds, which can be used as an adjuvant therapy and has well-known mechanisms for inhibiting cancer metastases, improving anti-cancer treatment, and enhancing immunity and antioxidant capacity. Our designed combination, named MVBL, consists of four inexpensive compounds: L-selenium-methylselenocysteine (MSC), D-‍α‍-tocopheryl succinic acid (VES),β‍-carotene (β‍-Ca), and L-lysine (Lys).
MethodsThe effects of MVBL on cell viability, cell cycle, cell apoptosis, cell migration, cell invasion, reactive oxygen species (ROS), and paclitaxel (PTX)-combined treatment were studied in vitro. The inhibition of tumor metastasis, antioxidation, and immune enhancement capacity of MVBL were determined in vivo.
ResultsMVBL exhibited higher toxicity to tumor cells than to normal cells. It did not significantly affect the cell cycle of cancer cells, but increased their apoptosis. Wound healing, adhesion, and transwell assays showed that MVBL significantly inhibited tumor cell migration, adhesion, and invasion. MVBL sensitized MDA-MB-231 breast cancer cells to PTX, indicating that it can be used as an adjuvant to enhance the therapeutic effect of chemotherapy drugs. In mice, experimental data showed that MVBL inhibited tumor metastasis, prolonged their survival time, and enhanced their antioxidant capacity and immune function.
ConclusionsThis study revealed the roles of MVBL in improving immunity and antioxidation, preventing tumor growth, and inhibiting metastasis in vitro and in vivo. MVBL may be used as an adjuvant drug in cancer therapy for improving the survival and quality of life of cancer patients.

L-Se-甲基硒代半胱氨酸、D-α-生育酚琥珀酸酯、β-胡萝卜素和L-赖氨酸联合作为辅助治疗手段可预防肿瘤转移

程云龙1,2,练殊1,3,李书慧2,卢余盛1,王杰2,邓潇潇2,翟胜益2,贾力1,2,3
1闽江学院材料与化学工程学院智能药物闽台港澳科技合作基地,福建福州,350108
2福州大学化学学院,肿瘤转移预警与预防研究中心,福建省肿瘤转移化学预防重点实验室,福建福州,350002
3闽江学院福州海洋研究院,福建福州,350108
目的:通过手术切除和辅助疗法治疗原发性肿瘤已被广泛研究,但对于肿瘤转移的有效治疗策略和药物仍然缺乏。本研究中,我们提出了一种基于组合化合物的功能性产品用作肿瘤辅助治疗,各化合物机制明确,可抑制癌症转移、改善抗癌治疗、增强免疫力和抗氧化。该组合由四种廉价化合物组成:L-硒-甲基硒代半胱氨酸(MSC)、D-α-生育酚琥珀酸(VES)、β-胡萝卜素(β-Ca)和赖氨酸(Lys),命名为MVBL。
方法:通过体外实验研究MVBL对细胞活力、细胞周期、细胞凋亡、细胞迁移、细胞侵袭、活性氧(ROS)和紫杉醇(PTX)联合治疗的影响。在动物水平测定了MVBL在体内的抗氧化和免疫增强能力。通过小鼠肿瘤模型测定MVBL在体内对肿瘤转移的抑制。
结果:MVBL对肿瘤细胞的毒性高于对正常细胞的毒性。它没有显著性地影响癌细胞的细胞周期,但增加了细胞凋亡。划痕、粘附和侵袭实验结果表明,MVBL显著抑制肿瘤细胞迁移、粘附和侵袭。MVBL使乳腺癌MDA-MB-231细胞对PTX更敏感,表明它可以作为佐剂来增强化疗药物的治疗效果。小鼠实验数据表明,MVBL可以抑制肿瘤转移,延长其生存时间,增强其抗氧化能力和免疫功能。
结论:本研究揭示了MVBL在提高免疫、抗氧化、防止肿瘤生长和抑制体内外转移方面的作用。MVBL可用作癌症治疗的辅助药物,提高癌症患者的生存率和生活质量。

关键词:肿瘤预防;肿瘤转移;硒;联合用药;营养

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Abu SamaanTM, SamecM, LiskovaA, et al., 2019. Paclitaxel’s mechanistic and clinical effects on breast cancer. Biomolecules, 9(12):789.

[2]AveryJC, HoffmannPR, 2018. Selenium, selenoproteins, and immunity. Nutrients, 10(9):1203.

[3]BarrettM, Uí DhuibhirP, NjorogeC, et al., 2020. Diet and nutrition information on nine national cancer organisation websites: a critical review. Eur J Cancer Care (Engl), 29(5):e13280.

[4]CheungEC, DenicolaGM, NixonC, et al., 2020. Dynamic ROS control by TIGAR regulates the initiation and progression of pancreatic cancer. Cancer Cell, 37(2):168-182.e4.

[5]CrowderSW, HortonLW, LeeSH, et al., 2013. Passage-dependent cancerous transformation of human mesenchymal stem cells under carcinogenic hypoxia. FASEB J, 27(7):2788-2798.

[6]DikicI, ElazarZ, 2018. Mechanism and medical implications of mammalian autophagy. Nat Rev Mol Cell Biol, 19(6):349-364.

[7]FangYZ, YangS, WuGY, 2002. Free radicals, antioxidants, and nutrition. Nutrition, 18(10):872-879.

[8]GanashMA, 2021. Anticancer potential of ascorbic acid and inorganic selenium on human breast cancer cell line MCF-7 and colon carcinoma HCT-116. J Cancer Res Ther, 17(1):122-129.

[9]GuoYY, XiaoYY, GuoHC, et al., 2021. The anti-dysenteric drug fraxetin enhances anti-tumor efficacy of gemcitabine and suppresses pancreatic cancer development by antagonizing STAT3 activation. Aging (Albany NY), 13(14):18545-18563.

[10]HébertJR, HurleyTG, SteckSE, et al., 2014. Considering the value of dietary assessment data in informing nutrition-related health policy. Adv Nutr, 5(4):447-455.

[11]HuangXL, NeckenigM, SunJT, et al., 2021. Vitamin E succinate exerts anti-tumour effects on human cervical cancer cells via the CD47-SIRPα pathway both in vivo and in vitro. J Cancer, 12(13):3877-3886.

[12]Ibrahim-HashimA, WojtkowiakJW, de Lourdes Coelho RibeiroM, et al., 2011. Free base lysine increases survival and reduces metastasis in prostate cancer model. J Cancer Sci Ther, Suppl 1(4):JCST-S1-004.

[13]JiangQ, 2017. Natural forms of vitamin E as effective agents for cancer prevention and therapy. Adv Nutr, 8(6):850-867.

[14]JiangZW, ChiJH, LiH, et al., 2021. Effect of chitosan oligosaccharide-conjugated selenium on improving immune function and blocking gastric cancer growth. Eur J Pharmacol, 891:173673.

[15]KimYS, LeeHA, LimJY, et al., 2014. β-Carotene inhibits neuroblastoma cell invasion and metastasis in vitro and in vivo by decreasing level of hypoxia-inducible factor-1α. J Nutr Biochem, 25(6):655-664.

[16]KirchhainA, ZubrienėA, KairysV, et al., 2021. Biphenyl substituted lysine derivatives as recognition elements for the matrix metalloproteinases MMP-2 and MMP-9. Bioorg Chem, 115:105155.

[17]KontekR, JakubczakM, Matlawska-WasowskaK, 2014. The antioxidants, vitamin A and E but not vitamin C and melatonin enhance the proapoptotic effects of irinotecan in cancer cells in vitro. Toxicol in Vitro, 28(2):282-291.

[18]LiCH, LiYF, YaoTT, et al., 2020. Wireless electrochemotherapy by selenium-doped piezoelectric biomaterials to enhance cancer cell apoptosis. ACS Appl Mater Interfaces, 12(31):34505-34513.

[19]LiuXY, PeiCY, YanS, et al., 2015. NADPH oxidase 1-dependent ROS is crucial for TLR4 signaling to promote tumor metastasis of non-small cell lung cancer. Tumor Biol, 36(3):1493-1502.

[20]MaL, LiuL, MaYC, et al., 2017. The role of E-cadherin/β-catenin in hydroxysafflor yellow a inhibiting adhesion, invasion, migration and lung metastasis of hepatoma cells. Biol Pharm Bull, 40(10):1706-1715.

[21]MarianM, AugustDA, 2014. Prevalence of malnutrition and current use of nutrition support in cancer patient study. J Parenter Enteral Nutr, 38(2):163-165.

[22]Martínez-ReyesI, ChandelNS, 2021. Cancer metabolism: looking forward. Nat Rev Cancer, 21(10):669-680.

[23]ØrskovH, FlyvbjergA, 2000. Selenium and human health. Lancet, 356(9233):942-943.

[24]PrasadS, GuptaSC, TyagiAK, 2017. Reactive oxygen species (ROS) and cancer: role of antioxidative nutraceuticals. Cancer Lett, 387:95-105.

[25]RoomiMW, IvanovV, KalinovskyT, et al., 2005. In vitro and in vivo antitumorigenic activity of a mixture of lysine, proline, ascorbic acid, and green tea extract on human breast cancer lines MDA-MB-231 and MCF-7. Med Oncol, 22(2):129-138.

[26]SatomiY, NishinoH, 2013. Inhibition of the enzyme activity of cytochrome P450 1A1, 1A2 and 3A4 by fucoxanthin, a marine carotenoid. Oncol Lett, 6(3):860-864.

[27]ShimojoY, AkimotoM, HisanagaT, et al., 2013. Attenuation of reactive oxygen species by antioxidants suppresses hypoxia-induced epithelial-mesenchymal transition and metastasis of pancreatic cancer cells. Clin Exp Metastasis, 30(2):143-154.

[28]SiegelRL, MillerKD, FuchsHE, et al., 2022. Cancer statistics, 2022. CA Cancer J Clin, 72(1):7-33.

[29]SuX, ShenZ, YangQ, et al., 2019. Vitamin C kills thyroid cancer cells through ROS-dependent inhibition of MAPK/ERK and PI3K/AKT pathways via distinct mechanisms. Theranostics, 9(15):4461-4473.

[30]WangYW, QiH, LiuY, et al., 2021. The double-edged roles of ROS in cancer prevention and therapy. Theranostics, 11(10):4839-4857.

[31]ZhangT, ZhuXY, WuHC, et al., 2019. Targeting the ROS/PI3K/AKT/HIF-1α/HK2 axis of breast cancer cells: combined administration of Polydatin and 2-Deoxy-d-glucose. J Cell Mol Med, 23(5):3711-3723.

[32]ZhouMG, WangHD, ZengXY, et al., 2019. Mortality, morbidity, and risk factors in China and its provinces, 1990‒2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet, 394(10204):1145-1158.

[33]ZhouX, JiWJ, ZhuY, et al., 2007. Enhancement of endogenous defenses against ROS by supra-nutritional level of selenium is more safe and effective than antioxidant supplementation in reducing hypertensive target organ damage. Med Hypotheses, 68(5):952-956.

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