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Received: 2023-06-12

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Journal of Zhejiang University SCIENCE B 2024 Vol.25 No.3 P.233-243


Endoplasmic reticulum stress-induced NLRP3 inflammasome activation as a novel mechanism of polystyrene microplastics (PS-MPs)-induced pulmonary inflammation in chickens

Author(s):  Hongmin LU, Tiantian GUO, Yue ZHANG, Dewang LIU, Lulu HOU, Chengxue MA, Mingwei XING

Affiliation(s):  College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China

Corresponding email(s):   mch007@vip.163.com, xingmingwei@nefu.edu.cn

Key Words:  Polystyrene microplastics, Endoplasmic reticulum stress, Lung, NLRP3 inflammasome, Inflammation

Hongmin LU, Tiantian GUO, Yue ZHANG, Dewang LIU, Lulu HOU, Chengxue MA, Mingwei XING. Endoplasmic reticulum stress-induced NLRP3 inflammasome activation as a novel mechanism of polystyrene microplastics (PS-MPs)-induced pulmonary inflammation in chickens[J]. Journal of Zhejiang University Science B, 2024, 25(3): 233-243.

@article{title="Endoplasmic reticulum stress-induced NLRP3 inflammasome activation as a novel mechanism of polystyrene microplastics (PS-MPs)-induced pulmonary inflammation in chickens",
author="Hongmin LU, Tiantian GUO, Yue ZHANG, Dewang LIU, Lulu HOU, Chengxue MA, Mingwei XING",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Endoplasmic reticulum stress-induced NLRP3 inflammasome activation as a novel mechanism of polystyrene microplastics (PS-MPs)-induced pulmonary inflammation in chickens
%A Hongmin LU
%A Tiantian GUO
%A Dewang LIU
%A Lulu HOU
%A Chengxue MA
%A Mingwei XING
%J Journal of Zhejiang University SCIENCE B
%V 25
%N 3
%P 233-243
%@ 1673-1581
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300409

T1 - Endoplasmic reticulum stress-induced NLRP3 inflammasome activation as a novel mechanism of polystyrene microplastics (PS-MPs)-induced pulmonary inflammation in chickens
A1 - Hongmin LU
A1 - Tiantian GUO
A1 - Yue ZHANG
A1 - Dewang LIU
A1 - Lulu HOU
A1 - Chengxue MA
A1 - Mingwei XING
J0 - Journal of Zhejiang University Science B
VL - 25
IS - 3
SP - 233
EP - 243
%@ 1673-1581
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300409

Microplastics (MPs) have attracted growing attention worldwide as an increasingly prevalent environmental pollutant. In addition, chicken meat is currently the most widely consumed kind of poultry in the global market. Consumer demand for chicken is on the rise both at home and abroad. As a result, the safety of chicken raising has also received significant attention. The lungs play an essential role in the physiological activities of chickens, and they are also the most vulnerable organs. lung injury is difficult to repair after the accumulation of contaminants, and the mortality rate is high, which brings huge economic losses to farmers. The research on the toxicity of MPs has mainly focused on the marine ecosystem, while the mechanisms of toxicity and lung damage in chickens have been poorly studied. Thus, this study explored the effects of exposure to polystyrene microplastics (PS-MPs) at various concentrations for 42 d on chicken lungs. PS-MPs could cause lung pathologies and ultrastructural abnormalities, such as endoplasmic reticulum (ER) swelling, inflammatory cell infiltration, chromatin agglutination, and plasma membrane rupture. Simultaneously, PS-MPs increased the expression of genes related to the heat shock protein family (Hsp60, Hsp70, and Hsp90), ER stress signaling (activating transcription factor 6 (ATF6), ATF4, protein kinase RNA-like ER kinase (PERK), and eukaryotic translation initiation factor 2 subunit α (eIF2α)), pyroptosis-related genes (NOD-‍, LRR- and pyrin domain-containing protein 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), interleukin-1β (IL-1β), cysteinyl aspartate-specific proteinase 1 (Caspase1), and gasdermin-D (GSDMD)), and the inflammatory signaling pathway (nuclear factor-‍κB (NF-κB), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2)). The above results showed that PS-MP exposure could result in lung stress, ER stress, pyroptosis, and inflammation in broilers. Our findings provide new scientific clues for further research on the mechanisms of physical health and toxicology regarding MPs.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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