Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.2 P.173-180

In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysacchaides from fruit peel of tea (Camellia sinensis L.)* #

Author(s): Yue-fei Wang^1,2,Jie Wang¹,Jing Wu¹,Ping Xu^1,2,Yi-qi Wang³,Jun-jie Gao¹,Danielle Hochstetter¹
Affiliation(s): 1. 1. Department of Tea Science, Zhejiang University, Hangzhou 310058, China more
Corresponding email(s): zdxp@zju.edu.cn
Key Words: Polysaccharides, Tea (Camellia sinensis L.) fruit peel, Physicochemical properties, Antioxidant activity, &alpha,-Glucosidase inhibition

Share this article to： More <<< Previous Article \|Next Article >>>

Yue-fei Wang, Jie Wang, Jing Wu, Ping Xu, Yi-qi Wang, Jun-jie Gao, Danielle Hochstetter. In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.)[J]. Journal of Zhejiang University Science B, 2014, 15(2): 173-180.

@article{title="In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.)",
author="Yue-fei Wang, Jie Wang, Jing Wu, Ping Xu, Yi-qi Wang, Jun-jie Gao, Danielle Hochstetter",
journal="Journal of Zhejiang University Science B",
volume="15",
number="2",
pages="173-180",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300186"
}

%0 Journal Article
%T In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.)
%A Yue-fei Wang
%A Jie Wang
%A Jing Wu
%A Ping Xu
%A Yi-qi Wang
%A Jun-jie Gao
%A Danielle Hochstetter
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 2
%P 173-180
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300186

TY - JOUR
T1 - In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.)
A1 - Yue-fei Wang
A1 - Jie Wang
A1 - Jing Wu
A1 - Ping Xu
A1 - Yi-qi Wang
A1 - Jun-jie Gao
A1 - Danielle Hochstetter
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 2
SP - 173
EP - 180
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300186

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied. Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM). Under the optimized conditions, the yield of TFPP was predicted to be 4.98%. The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated. We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions. They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro. Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.

茶果皮多糖理化性质、体外抗氧化活性及对α-葡萄糖苷酶的抑制作用

研究目的：利用响应面优化茶果皮多糖（TFPP）提取条件，用乙醇分级分段得到4个多糖组分（TFPP-0、TFPP-20、TFPP-40和TFPP-60），并研究其理化性质、抗氧化活性和对α-葡萄糖苷酶抑制作用，为综合高效利用茶果皮多糖资源提供理论基础。
创新要点：1. 首次将茶果皮作为一种潜在生物资源研究；2. 首次研究茶果皮多糖这一功能成分；3. 将工艺优化、理化性质和生物活性结合研究。
研究方法：三因素三水平响应面设计（见表1），傅里叶转换红外光谱法分析茶果皮粗多糖的功能团结构（见图3），高效液相色谱法检测单糖组分（见表2），2,2′-氨基-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐（ABTS）自由基清除法（见图 4a）和铁离子还原能力法（FRAP）（见图4b）分析茶果皮多糖抗氧化活性。
重要结论：1. 茶果皮多糖是一种水溶性的酸性杂多糖蛋白复合物；2. 乙醇分级是一种有效多糖分离手段；3. 茶果皮多糖具有出色的生物活性；4. 茶果皮资源可以作为一种可再生生物资源进行深度的开发。

关键词：茶果皮；多糖；响应面；抗氧化；α-葡萄糖苷酶抑制