Full Text:   <1513>

CLC number: 

On-line Access: 2020-07-18

Received: 2020-04-24

Revision Accepted: 2020-06-26

Crosschecked: 2020-08-29

Cited: 0

Clicked: 1741

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Bio-Design and Manufacturing  2020 Vol.3 No.4 P.396-409

http://doi.org/10.1007/s42242-020-00086-4


Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scafold fabricated by 3D printing


Author(s):  Qiongxi Pan, Chenyuan Gao, Yingying Wang, Yili Wang, Cong Mao, Quan Wang, Sophia N. Economidou, Dennis Douroumis, Feng Wen, Lay Poh Tan & Huaqiong Li

Affiliation(s):  School of Biomedical Engineering, School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, People’s Republic of China; more

Corresponding email(s):   lptan@ntu.edu.sg, lihq@wiucas.ac.cn

Key Words:  Polycaprolactone, 3D printing, Decellularized porcine bone, Cranial bone regeneration, Attenuated immunogenicity


Share this article to: More

Qiongxi Pan, Chenyuan Gao, Yingying Wang, Yili Wang, Cong Mao, Quan Wang, Sophia N. Economidou, Dennis Douroumis, Feng Wen, Lay Poh Tan & Huaqiong Li . Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scafold fabricated by 3D printing[J]. Journal of Zhejiang University Science D, 2020, 3(4): 396-409.

@article{title="Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scafold fabricated by 3D printing",
author="Qiongxi Pan, Chenyuan Gao, Yingying Wang, Yili Wang, Cong Mao, Quan Wang, Sophia N. Economidou, Dennis Douroumis, Feng Wen, Lay Poh Tan & Huaqiong Li ",
journal="Journal of Zhejiang University Science D",
volume="3",
number="4",
pages="396-409",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00086-4"
}

%0 Journal Article
%T Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scafold fabricated by 3D printing
%A Qiongxi Pan
%A Chenyuan Gao
%A Yingying Wang
%A Yili Wang
%A Cong Mao
%A Quan Wang
%A Sophia N. Economidou
%A Dennis Douroumis
%A Feng Wen
%A Lay Poh Tan & Huaqiong Li
%J Journal of Zhejiang University SCIENCE D
%V 3
%N 4
%P 396-409
%@ 1869-1951
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00086-4

TY - JOUR
T1 - Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scafold fabricated by 3D printing
A1 - Qiongxi Pan
A1 - Chenyuan Gao
A1 - Yingying Wang
A1 - Yili Wang
A1 - Cong Mao
A1 - Quan Wang
A1 - Sophia N. Economidou
A1 - Dennis Douroumis
A1 - Feng Wen
A1 - Lay Poh Tan & Huaqiong Li
J0 - Journal of Zhejiang University Science D
VL - 3
IS - 4
SP - 396
EP - 409
%@ 1869-1951
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00086-4


Abstract: 
Bone is known to have a natural function to heal itself. However, if the bone damage is beyond a critical degree, intervention such as bone grafting may be imperative. In this work, the fabrication of a novel bone scafold composed of natural bone components and polycaprolactone (PCL) using 3D printing is put forward. α1, 3-galactosyltransferase defcient pigs were used as the donor source of a xenograft. decellularized porcine bone (DCB) with attenuated immunogenicity was used as the natural component of the scafold with the aim to promote bone regeneration. The 3D printed DCB-PCL scafolds combined essential advantages such as uniformity of the interconnected macropores and high porosity and enhanced compressive strength. The biological properties of the DCB-PCL scafolds were evaluated by studying cell adhesion, viability, alkaline phosphatase activity and osteogenic gene expression of human bone marrow-derived mesenchymal stem cells. The in vitro results demonstrated that the DCB-PCL scafolds exhibit an enhanced performance in promoting bone diferentiation, which is correlated to the DCB content. Furthermore, critical-sized cranial rat defects were used to assess the efect of DCB-PCL scafolds on bone regeneration in vivo. The results confrm that in comparison with PCL scafolds, the DCB-PCL scafolds can signifcantly improve new bone formation in cranial defects. Thus, the proposed 3D printed DCB-PCL scafolds emerge as a promising regeneration alternative in the clinical treatment of large bone defects.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE