JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE A 2002 Vol.3 No.5 P.622-626

http://doi.org/10.1631/jzus.2002.0622

Use of flow cytometry to investigate the cytokine response pattern in infants with respiratory syncytial virus infection and bronchiolitis

Author(s): CHEN Zhi-min, DU Li-zhong, MAO Jian-hua, TANG Yong-min
Affiliation(s): The Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China
Corresponding email(s): chenzm@mail.hz.zj.cn
Key Words: Respiratory syncytial virus (RSV), Bronchiolitis, IL-4/IFN-γ

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Abstract: Objective: To investigate the cytokine response pattern (IL-4/IFN-γ;) in infants with RSV infections and bronchiolitis during the acute phase. Methods: Four-color flow cytometry was used to measure intracellular IL-4 and IFN-γ expressions in peripheral blood CD3+ and CD8+ lymphocytes from RSV-infected and bronchiolitis infants. Serum IL-4 and IFN-γ levels were also determined. Results: RSV-infected and bronchiolitis infants showed no statistical differences from not-RSV-infected or pneumonia infants and control in the frequency of IL-4 and IFN-γ expressions in CD3+CD8- lymphocytes, showed no obvious Th1/Th2 imbalance, while IFN-γ was expressed much more frequently in CD3+CD8+ lymphocytes. Systematically, RSV-infected and bronchiolitis infants showed much lower levels of serum IL-4 and IL-4/IFN-γ; ratios and much higher serum IFN-γ levels than control. However, there were no statistical differences in the above three indices between RSV-infected and not-RSV infected infants or between bronchiolitis and pneumonia infants, except that bronchiolitis infants had a higher level of serum IFN-γ than pneumonia infants statistically. Conclusions: There is no type-2 cytokine response predominance in the acute phase of RSV infection and bronchiolitis. IL-4 production is suppressed and IFN-γ production upregulated, the latter being most prominent in bronchiolitis infants.

Reference

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