JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.2 P.135-142

Evaluation of the hepatoprotective and antioxidant activities of Rubus parvifolius L.

Author(s): Jie Gao, Cui-rong Sun, Jie-hong Yang, Jian-mei Shi, Yue-guang Du, Yu-yan Zhang, Jin-hui Li, Hai-tong Wan
Affiliation(s): Department of Chemistry, Zhejiang University, Hangzhou 310027, China; more
Corresponding email(s): suncuirong@zju.edu.cn, yannoo7376@sina.com
Key Words: Rubus parvifolius L., High-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS), Hepatoprotective activity, Antioxidant activity

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Abstract: The hepatoprotective and antioxidant activities of the n-butanol extract of Rubus parvifolius L. (RPL), a widely used medicinal plant, were evaluated. Results demonstrated that RPL extract possessed pronounced hepatoprotective effects against carbon tetrachloride (CCl₄)-induced hepatic injury in mice, which was at least partially attributed to its strong antioxidant capacity. Treatment with RPL extract markedly attenuated the increases in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels caused by CCl₄ intoxication. It also significantly prevented the decrease in superoxide dismutase (SOD) activity and the increase in malondialdehyde (MDA) content of liver tissue. Meanwhile, histopathological changes of hepatic damage were also remarkably ameliorated. Phytochemical analysis based on high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) revealed the presence of various phenolic compounds, including caffeic acid conjugates, ellagic acid glycosides, and flavonol glycosides, which might be responsible for the hepatoprotective and antioxidant activities of RPL.

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