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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.12 P.1613~1620

http://doi.org/10.1631/jzus.2004.1613


Mathematical modeling of salt-gradient ion-exchange simulated moving bed chromatography for protein separations


Author(s):  LU Jian-gang

Affiliation(s):  National Laboratory of Industrial Control Technology, Institute of Industrial Process Control, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   JGLU@iipc.zju.edu.cn

Key Words:  Simulated moving bed chromatography, Salt gradients, Size exclusion, Proteins, Mathematical model


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LU Jian-gang. Mathematical modeling of salt-gradient ion-exchange simulated moving bed chromatography for protein separations[J]. Journal of Zhejiang University Science A, 2004, 5(12): 1613~1620.

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author="LU Jian-gang",
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.1613

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T1 - Mathematical modeling of salt-gradient ion-exchange simulated moving bed chromatography for protein separations
A1 - LU Jian-gang
J0 - Journal of Zhejiang University Science A
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.1613


Abstract: 
The salt-gradient operation mode used in ion-exchange simulated moving bed chromatography (SMBC) can improve the efficiency of protein separations. A detailed model that takes into account any kind of adsorption/ion-exchange equilibrium, salt gradient, size exclusion, mass transfer resistance, and port periodic switching mechanism, was developed to simulate the complex dynamics. The model predictions were verified by the experimental data on upward and downward gradients for protein separations reported in the literature. All design and operating parameters (number, configuration, length and diameter of columns, particle size, switching period, flow rates of feed, raffinate, desorbent and extract, protein concentrations in feed, different salt concentrations in desorbent and feed) can be chosen correctly by numerical simulation. This model can facilitate the design, operation, optimization, control and scale-up of salt-gradient ion-exchange SMBC for protein separations.

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Reference

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