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Received: 2004-01-16
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Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS
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PAN Chun-xiu, XU Xiu-zhu, HE Hong-mei, CAI Xiao-jun, ZHANG Xue-jun. Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS[J]. Journal of Zhejiang University Science B, 2005, 6(1): 74-78.
@article{title="Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS",
author="PAN Chun-xiu, XU Xiu-zhu, HE Hong-mei, CAI Xiao-jun, ZHANG Xue-jun",
journal="Journal of Zhejiang University Science B",
volume="6",
number="1",
pages="74-78",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0074"
}
%0 Journal Article
%T Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS
%A PAN Chun-xiu
%A XU Xiu-zhu
%A HE Hong-mei
%A CAI Xiao-jun
%A ZHANG Xue-jun
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 1
%P 74-78
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0074
TY - JOUR
T1 - Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS
A1 - PAN Chun-xiu
A1 - XU Xiu-zhu
A1 - HE Hong-mei
A1 - CAI Xiao-jun
A1 - ZHANG Xue-jun
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 1
SP - 74
EP - 78
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0074
Abstract: The cis and trans isomers separation of 2-butene-1,4-diol and lafutidine were studied by HPLC on two kinds of chiral columns: (S,s)-Whelk-O 1 and chiraSpher. The isomers of 2-butene-1,4-diol can be separated on both chiral columns while the isomers of lafutidine can only be resolved on chiraSpher column. The influence of different type and amount of mobile phase modifier on the isomers separation was extensively studied. The resolution of cis and trans isomers of 2-butene-1,4-diol was 2.61 on (S,s)-Whelk-O 1 column with hexane-ethanol (97:3, v/v) as the mobile phase. The resolution of lafutidine was 1.89 on chiraSpher column with hexane-ethanol-THF-diethylamine (92:3:5:0.1, v/v/v/v) as the mobile phase. LC-MS methods were developed to identify the isomer peaks.
[1] Boonen, G., Beck, M., Häberlein, H., 1997. Contribution to the quantitative and enantioselective determination of kavapyrones by high-performance liquid chromatography on ChiraSpher NT material. Journal of Chromatography B, 702:240.
[2] Chen, W.Q., Xu, D.Y., 2003. Lafutidine, a novel antiulcer drug. Chinese J. New Drugs Clin. Rem., 4:247-250 (in Chinese).
[3] Krause, K., Girod, M., Chankvetadze, B., Blaschke, G., 1999. Enantioseparations in normal- and reversed-phase nano-high-performance liquid chromatography and capillary electrochromatography using polyacrylamide and polysaccharide derivatives as chiral stationary phases. J. Chromatogr. A, 837:51-63.
[4] Onodera, S., Shibata, M., Tanaka, M., Inaba, N., Yamamura, T., Ohnishi, H., 1995. Gastroprotective activity of FRG-8813, a novel histamine H2-receptor antagonist, in rats. Jpn. J. Pharmacol., 68:161-173.
[5] Pirkle, W.H., Welch, C.J., 1992. An improved chiral stationary phase for the chromatographic-separation of underivatized naproxen enantiomers. J. Liq. Chromatogr., 15:1947-1955.
[6] Schulte, M., Devent, R., Grosser, R., 2002. Enantioseparation of Gantofiban precursors on chiral stationary phases of the poly-(N-acryloyl amino acid derivative)-type. Journal of Pharmaceutical and Biomedical Analysis, 27:627-637.
[7] Shao, B.H., Xu, X.Z., Lu, J.D., Cai, X.J., Zou, L., Fu, X.Y., 2003. Comparison of enantioseparation and chiral recognition mechanism of racemic naproxen esters on (S,S)-whelk-O 1 and CDMPC chiral columns. Acta Chim. Sinica, 61:1635-1640.
[8] Shibata, M., Yamamura, T., Inaba, N., Onodera, S., Chiba, Y., Ohnishi, H., 1993. Gastric antisecretory effect of FRG-8813, a new histamine H2-receptor antagonist, in rats and dogs. Eur J. Pharmacol., 235:245-253.
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