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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.1 P.74~78

http://doi.org/10.1631/jzus.2005.B0074


Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS


Author(s):  PAN Chun-xiu, XU Xiu-zhu, HE Hong-mei, CAI Xiao-jun, ZHANG Xue-jun

Affiliation(s):  Department of Chemistry, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   xuxiuzhu@zju.edu.cn

Key Words:  2-butene-1, 4-diol, Lafutidine, Isomers separation, (S, S)-Whelk-O 1, ChiraSpher, LC-MS


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PAN Chun-xiu, XU Xiu-zhu, HE Hong-mei, CAI Xiao-jun, ZHANG Xue-jun. Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS[J]. Journal of Zhejiang University Science B, 2005, 6(1): 74~78.

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author="PAN Chun-xiu, XU Xiu-zhu, HE Hong-mei, CAI Xiao-jun, ZHANG Xue-jun",
journal="Journal of Zhejiang University Science B",
volume="6",
number="1",
pages="74~78",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0074"
}

%0 Journal Article
%T Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS
%A PAN Chun-xiu
%A XU Xiu-zhu
%A HE Hong-mei
%A CAI Xiao-jun
%A ZHANG Xue-jun
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 1
%P 74~78
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0074

TY - JOUR
T1 - Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS
A1 - PAN Chun-xiu
A1 - XU Xiu-zhu
A1 - HE Hong-mei
A1 - CAI Xiao-jun
A1 - ZHANG Xue-jun
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 1
SP - 74
EP - 78
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0074


Abstract: 
The cis and trans isomers separation of 2-butene-1,4-diol and lafutidine were studied by HPLC on two kinds of chiral columns: (S,s)-Whelk-O 1 and chiraSpher. The isomers of 2-butene-1,4-diol can be separated on both chiral columns while the isomers of lafutidine can only be resolved on chiraSpher column. The influence of different type and amount of mobile phase modifier on the isomers separation was extensively studied. The resolution of cis and trans isomers of 2-butene-1,4-diol was 2.61 on (S,s)-Whelk-O 1 column with hexane-ethanol (97:3, v/v) as the mobile phase. The resolution of lafutidine was 1.89 on chiraSpher column with hexane-ethanol-THF-diethylamine (92:3:5:0.1, v/v/v/v) as the mobile phase. LC-MS methods were developed to identify the isomer peaks.

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Reference

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