JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.6 P.448-454

Effects of the crude extract of Polygala tenuifolia Willd on human sperm in vitro

Author(s): Yi Qiu, Lei-guang Wang, Yi-fang Jia, Dan-tong Yang, Mei-hua Zhang, Yan-ping Zhang, Li-hong Zhang, Ling Gai
Affiliation(s): Key Laboratory for Improving Birth Outcome Technique, Shandong Provincial Institute of Science and Technology for Family Planning, Jinan 250002, China
Corresponding email(s): qiuyi987@sina.com, qiuyimail@yahoo.com
Key Words: Polygala tenuifolia Willd, Crude extract, Spermicidal effect in vitro, Sperm membrane, DNA integrity

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Abstract: The aim of the present study is to analyze sperm membrane changes and the spermicidal effect in treatment with the crude extract from Polygala tenuifolia Willd (PTW) in vitro. The root of PTW was extracted in distilled water. Normal human spermatozoa were used to assess the spermicidal activity (Sander-Cramer assay) of the extract from the PTW root. The hypo-osmotic swelling (HOS) test and the eosin Y (EY) staining were used to detect the integrity of sperm membrane and vitality. The sperm chromatin dispersion (SCD) test was performed to determine sperm DNA integrity. N-9 was used as a reference standard and semen added to physiological saline was used as the control. Semen samples were donated by 42 healthy fertile men. The crude extract from the root of PTW could immobilize and kill 100% spermatozoa within 20 s in vitro at the concentrations of 20.0 and 10.0 mg/ml; at the concentration of 5.0 mg/ml, spermatozoa were immobilized in (39.5±3.2) s. In the groups of the crude extract from the root of PTW and N-9 solution, the rate of the normal HOS (tails swollen) and the white head (unstained) was 0%, and the rate of the abnormal HOS (tails unswollen) and red head (stained) was 100%. Sperm DNA fragmentation showed no change in exposure to the crude extract from the root of PTW and N-9 solution. The sperm revival test did not show any spermatozoa that recovered their motilities. The rapid spermicidal activity of the crude extract from the root of PTW in vitro may occur by the disruption of the sperm membrane integrity.

Reference

[1]Baccetti, B., Collodel, G., Piomboni, P., 1996. Apoptosis in human ejaculated sperm cells (Notulae seminologicae 9). J. Submicrosc. Cytol. Pathol., 28(4):587-596.

[2]Björndahl, L., Barratt, C.L.R., Fraser, L., Kvist, U., Mortimer, D., 2002. ESHRE basic semen analysis courses 1995–1999: immediate beneficial effects of standardized training. Hum. Reprod., 17(5):1299-1305.

[3]Björndahl, L., Söderlund, I., Kvist, U., 2003. Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment. Hum. Reprod., 18(4):813-816.

[4]Blanc-Layrac, G., Bringuier, A.F., Guillot, R., Feldmann, G., 2000. Morphological and biochemical analysis of cell death in human ejaculated spermatozoa. Cell Mol. Biol., 46(1):187-197.

[5]Chakrabarti, K., Pal, S., Bhattacharyya, A.K., 2003. Sperm immobilization activity of Allium sativum L. and other plant extracts. Asian J. Androl., 5(3):131-135.

[6]Chan, E., Tan M., Xin, J., Sudarsanam, S., Johnson, D.E., 2010. Interactions between traditional Chinese medicines and Western therapeutics. Curr. Opin. Drug Discov. Devel., 13(1):50-65.

[7]Chijioke, P.C., Zaman, S., Pearson, R.M., 1986. Comparison of the potency of d-propranolol, chlorhexidine and nonoxynol-9 in the Sander-Cramer test. Contraception, 34(2):207-211.

[8]Dott, H.M., Foster, G.C., 1972. A technique for studying the morphology of mammalian spermatozoa which are eosinophilic in a differential live/dead stain. J. Reprod. Fertil., 29(3):443-445.

[9]Fernández, J.L., Muriel, L., Rivero, M.T., Goyanes, V., Vazquez, R., Alvarez, J.G., 2003. The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J. Androl., 24(1):59-66.

[10]Gandini, L., Lombardo, F., Paoli, D., Caponecchia, L., Familiari, G., Verlengia, C., Dondero, F., Lenzi, A., 2000. Study of apoptotic DNA fragmentation in human spermatozoa. Hum. Reprod., 15(4):830-839.

[11]Gao, J., Sun, C.R., Yang, J.H., Shi, J.M., Du, Y.G., Zhang, Y.Y., Li, J.H., Wan, H.T., 2011. Evaluation of the hepatoprotective and antioxidant activities of Rubus parvifolius L. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 12(2):135-142.

[12]Huang, D., Li, J., He, L.Q., 2009. Influence of Tripterygium wilfordii on the expression of spermiogenesis related genes Herc4, Ipo11 and Mrto4 in mice. Hereditas, 31(9):941-946 (in Chinese).

[13]Jeyendran, R.S., van der Ven, H.H., Perez-Pelaez, M., Crabo, B.G., Zaneveld, L.J., 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70(1):219-228.

[14]Kamischke, A., Nieschlag, E., 1999. Treatment of retrograde ejaculation and anejaculation. Hum. Reprod. Update, 5(5):448-474.

[15]Laforest, G., Serqerie, M., Bleau, G., 2004. Innocuity of tetradecyl-dimethyl-benzyl ammonium fluoride on the DNA of human spermatozoa. Contraception, 69(5):425-432.

[16]Li, J., Huang, D., He, L.Q., 2009. Effects of glycosides of Tripterygium wilfordii and Cistanche deserticola on the fertility of male mice. Natl. J. Androl., 15(6):569-572 (in Chinese).

[17]Liu, Y.X., 2005. Control of spermatogenesis in primate and prospect of male contraception. Arch. Androl., 51(2):77-92.

[18]Lohiya, N.K., Kothari, L.K., Manivannan, B., Mishra, P.K., Pathak, N., 2000. Human sperm immobilization effect of Carica papaya seed extracts: an in vitro study. Asian J. Androl., 2(2):103-109.

[19]Maione, B., Pittoggi, C., Achene, L., Lorenzini, R., Spadafora, C., 1997. Activation of endogenous nucleases in mature sperm cells upon interaction with exogenous DNA. DNA Cell Biol., 16(9):1087-1097.

[20]Matson, P., Kappelle, W., Malecki, I., 2009. The use of a hypo-osmotic swelling (HOS) test on sperm of the pig (Sus scrofa domesticus), emu (Dromaius novaehollandiae), Asian elephant (Elephas maximus), hamadryas baboon (Papio hamadryas hamadryas), and central rock rat (Zyzomys pedunculatus). Reprod. Biol., 9(2):181-187.

[21]Muriel, L., Goyanes, V., Segrelles, E., Gosálvez, J., Alvarez, J.G., Fernández, J.L., 2007. Increased aneuploidy rate in sperm with fragmented DNA as determined by the sperm chromatin dispersion (SCD) test and FISH analysis. J. Androl., 28(1):38-49.

[22]Primorac, M., Sekulovic, D., Antonic, S., 1985. In vitro determination of the spermicidal activity of plant saponins. Pharmazie, 40(8):585.

[23]Qian, S.Z., Wang, Z.G., 1984. Gossypol: a potential antifertility agent for males. Ann. Rev. Pharmacol. Toxicol., 24(1):329-360.

[24]Qiu, Y., Wang, L.G., Zhang, L.H., Yang, D.T., 2008. Analysis of sperm chromosomal abnormalities and sperm DNA fragmentation in infertile males. Chin. J. Med. Genet., 25(6):681-685 (in Chinese).

[25]Qiu, Y., Wang, L.G., Zhang, L.H., 2009. Effect of testis heat stress contraceptive underpants on sperm DNA integrity and sperm membrane damage of human male. Asian J. Androl., 11(5 Suppl.):159-160.

[26]Ramos, L., Wetzels, A.M.M., 2001. Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay. Hum. Reprod., 16(8):1703-1707.

[27]Sander, F.V., Cramer, S.D., 1941. Practical method for testing the spermicidal action of chemical contraceptives. Hum. Fertil., 6:134-137.

[28]Sharma, R.K., Pasqualotto, F.F., Nelson, D.R., Thomas, A.J.Jr., Agarwal, A., 1999. The reactive oxygen species-total antioxidant capacity score is a new measure of oxidative stress to predict male infertility. Hum. Reprod., 14(11):2801-2807.

[29]Souad, K., Ali, S., Mounir, A., Mounir, T.M., 2007. Spermicidal activity of extract from Cestrum parqui. Contraception, 75(2):152-156.

[30]Venma, P.K., Sharma, A., Mathur, A., Sharma, P., Gupta, R.S., Joshi, S.C., Dixit, V.P., 2002. Effect of Sarcostemma acidum stem extract on spermatogenesis in male albino rats. Asian J. Androl., 4(1):43-47.

[31]Waller, D.P., Zaneveld, L.J.D., Fong, H.H.S., 1980. In vitro spermicidal activity of gossypol. Contraception, 22(2):183-187.

[32]WHO (World Health Organization), 1999. Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 4th Ed. Cambridge University Press, Cambridge, New York, p.10-26.

[33]Zhang, L.H., Qiu, Y., Wang, K.H., Wang, Q.J., Tao, F.Z., Wang, L.G., 2010. Measurement of sperm DNA fragmentation using bright-field microscopy: comparison between sperm chromatin dispersion test and terminal uridine nickend labeling assay. Fertil. Steril., 94(3):1027-1032.

[34]Zhuang, Y., Xie, X., 2009. Primary studies of toxicity-reducing and efficacy-maintaining action of fungal fermentative products in Tripterygium wilfordii by a novel bi-directional solidstate fungal fermentation. China J. Chin. Meter. Med., 34(16):2083-2087 (in Chinese).

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