CLC number: R735.2
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2014-04-24
Cited: 2
Clicked: 5589
Yan-yan Shi, Mo Chen, Yue-xia Zhang, Jing Zhang, Shi-gang Ding. Expression of three essential antioxidants of Helicobacter pylori in clinical isolates[J]. Journal of Zhejiang University Science B, 2014, 15(5): 500-506.
@article{title="Expression of three essential antioxidants of Helicobacter pylori in clinical isolates",
author="Yan-yan Shi, Mo Chen, Yue-xia Zhang, Jing Zhang, Shi-gang Ding",
journal="Journal of Zhejiang University Science B",
volume="15",
number="5",
pages="500-506",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300171"
}
%0 Journal Article
%T Expression of three essential antioxidants of Helicobacter pylori in clinical isolates
%A Yan-yan Shi
%A Mo Chen
%A Yue-xia Zhang
%A Jing Zhang
%A Shi-gang Ding
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 5
%P 500-506
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300171
TY - JOUR
T1 - Expression of three essential antioxidants of Helicobacter pylori in clinical isolates
A1 - Yan-yan Shi
A1 - Mo Chen
A1 - Yue-xia Zhang
A1 - Jing Zhang
A1 - Shi-gang Ding
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 5
SP - 500
EP - 506
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300171
Abstract: Objective: Helicobacter pylori maintains long-term persistence in the host and combats oxidative stress via many antioxidant proteins, which are expected to be relevant to bacterial-associated gastric diseases. We aimed to investigate the expression of three essential antioxidants in H. pylori strains isolated from patients with different clinical outcomes. Methods: Forty H. pylori strains were isolated from endoscopic biopsy specimens of gastric mucosa from 13 patients with gastric cancer, 13 with peptic ulcer, and 14 with gastritis. The expression of thioredoxin 1 (Trx1), arginase (RocF), and alkyl hydroperoxide reductase (AhpC) in H. pylori was measured by real-time PCR. Comparisons among multiple sample sets were analyzed using a one-way ANOVA test. Pearson’s correlation test was used to assess relationships among multiple continuous variables. Results: Trx1 expression of H. pylori in gastric cancer and peptic ulcer tissues was higher than that in tissues with gastritis. RocF expression of H. pylori in gastric cancer tissues was higher than that in tissues exhibiting peptic ulcer and gastritis. However, we did not find any differences in AhpC expression in samples from patients with different clinical outcomes. The expression of Trx1 and RocF had a positive, linear correlation. The expression of Trx1 and AhpC had a positive correlation without a linear trend. We found no correlation between the expression of RocF and AhpC. Conclusions: Our observations indicate that the expression of Trx1 and RocF in H. pylori might be related to gastric carcinogenesis. In H. pylori, the expression of members of the antioxidant system may be correlated and relevant to gastric cancer.
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