Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.9 P.766-775

Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo*

Author(s): Jian Yin¹,Jian-ming Yang¹,Feng Zhang²,Peng Miao^1,3,Ying Lin¹,Ming-li Chen¹
Affiliation(s): 1. 1. CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academic of Sciences, Suzhou 215163, China more
Corresponding email(s): yinj@sibet.ac.cn
Key Words: Joint toxicity, Cadmium sulfate, &alpha,-Naphthoflavone, Zebrafish embryo, Oxidative stress

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Jian Yin, Jian-ming Yang, Feng Zhang, Peng Miao, Ying Lin, Ming-li Chen. Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo[J]. Journal of Zhejiang University Science B, 2014, 15(9): 766-775.

@article{title="Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo",
author="Jian Yin, Jian-ming Yang, Feng Zhang, Peng Miao, Ying Lin, Ming-li Chen",
journal="Journal of Zhejiang University Science B",
volume="15",
number="9",
pages="766-775",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400091"
}

%0 Journal Article
%T Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo
%A Jian Yin
%A Jian-ming Yang
%A Feng Zhang
%A Peng Miao
%A Ying Lin
%A Ming-li Chen
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 9
%P 766-775
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400091

TY - JOUR
T1 - Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo
A1 - Jian Yin
A1 - Jian-ming Yang
A1 - Feng Zhang
A1 - Peng Miao
A1 - Ying Lin
A1 - Ming-li Chen
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 9
SP - 766
EP - 775
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400091

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: This paper aims to evaluate the individual and joint toxicities of cadmium sulfate (CdSO₄) and α-Naphthoflavone (ANF) in zebrafish embryos. As a result, CdSO₄ caused both lethal and sub-lethal effects, such as 24 h post-fertilization (hpf) death and 72 hpf delayed hatching. However, ANF only caused sub-lethal effects, including 48 hpf cardiac edema and 72 hpf delayed hatching. Taking 24 hpf death and 48 hpf cardiac edema as endpoints, the toxicities of CdSO₄ and ANF were significantly enhanced by each other. Consistently, both CdSO₄ and ANF caused significant oxidative stress, including decreases in the reduced glutathione (GSH) level, inhibition of superoxide dismutase (SOD) activity, as well as increases in malondialdehyde (MDA) content in zebrafish embryos, but these mixtures produced much more significant alterations on the biomarkers. Co-treatment of CdSO₄ and ANF significantly down-regulated the mRNA level of multidrug resistance-associated protein (mrp) 1 and cytochrome P450 (cyp) 1a, which constituted the protective mechanisms for zebrafish embryos to chemical toxins. In conclusion, co-treatment of CdSO₄ and ANF exhibited a much more severe damage in zebrafish embryos than individual treatment. Meanwhile, production of oxidative stress and altered expression of mrp1 and cyp1a could be important components of such joint toxicity.

硫酸镉与α-萘黄酮对斑马鱼发育的个体毒性与联合毒性研究

研究目的：研究硫酸镉与α-萘黄酮对斑马鱼发育的个体毒性与联合毒性，并探讨其中的联合毒性机制。
创新要点：首次对硫酸镉与α-萘黄酮的联合毒性进行了研究，并探索了氧化应激、ATP结合盒式（ABC）转运蛋白及细胞色素P450蛋白（CYP）1A在化合物联合毒性中的作用。
研究方法：在不同时间点，依据形态学指标检测硫酸镉与α-萘黄酮单独使用与联合使用时对斑马鱼胚胎的致死与致畸性毒性。取样品，采用试剂盒检测不同处理状态下斑马鱼胚胎中谷胱甘肽（GSH）、超氧化物歧化酶（SOD）及丙二醛（MDA）三个氧化还原指标的改变，聚合酶链式反应（PCR）检测样品中多药耐药蛋白（mrp）1及cyp1a基因表达水平的改变。
重要结论：硫酸镉与α-萘黄酮的联用能够产生远大于个体且显著增强各自的毒性，而这种增强作用机理在于氧化应激压力的产生，以及两者合用下mrp1及cyp1a基因表达水平的改变。
联合毒性；硫酸镉；α-萘黄酮；斑马鱼胚胎；氧化应激压力