Full Text:   <926>

CLC number: 

On-line Access: 2020-12-08

Received: 2020-08-12

Revision Accepted: 2020-11-06

Crosschecked: 0000-00-00

Cited: 0

Clicked: 1157

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE C 1998 Vol.-1 No.-1 P.

http://doi.org/10.1631/FITEE.2000410


Enhanced epi-collection of scattered photons in nonlinear fluorescence microscopy by extended detection with a silicon photomultiplier array


Author(s):  Ru-heng SHI, Cheng JIN, Chi LIU, Ling-jie KONG

Affiliation(s):  State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China; more

Corresponding email(s):   konglj@tsinghua.edu.cn

Key Words:  Extended detection, Epi-collection, Nonlinear fluorescence microscopy, Silicon photomultiplier array


Ru-heng SHI, Cheng JIN, Chi LIU, Ling-jie KONG. Enhanced epi-collection of scattered photons in nonlinear fluorescence microscopy by extended detection with a silicon photomultiplier array[J]. Frontiers of Information Technology & Electronic Engineering, 1998, -1(-1): .

@article{title="Enhanced epi-collection of scattered photons in nonlinear fluorescence microscopy by extended detection with a silicon photomultiplier array",
author="Ru-heng SHI, Cheng JIN, Chi LIU, Ling-jie KONG",
journal="Frontiers of Information Technology & Electronic Engineering",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/FITEE.2000410"
}

%0 Journal Article
%T Enhanced epi-collection of scattered photons in nonlinear fluorescence microscopy by extended detection with a silicon photomultiplier array
%A Ru-heng SHI
%A Cheng JIN
%A Chi LIU
%A Ling-jie KONG
%J Journal of Zhejiang University SCIENCE C
%V -1
%N -1
%P
%@ 2095-9184
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/FITEE.2000410

TY - JOUR
T1 - Enhanced epi-collection of scattered photons in nonlinear fluorescence microscopy by extended detection with a silicon photomultiplier array
A1 - Ru-heng SHI
A1 - Cheng JIN
A1 - Chi LIU
A1 - Ling-jie KONG
J0 - Journal of Zhejiang University Science C
VL - -1
IS - -1
SP -
EP -
%@ 2095-9184
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/FITEE.2000410


Abstract: 
To maximize signal collection in nonlinear optical microscopy, non-descanned epi-detection is generally adopted for in vivo imaging. However, because of severe scattering in biological samples, most of the emitted fluorescence photons go beyond the collection angles of objectives and thus cannot be detected. Here, we propose an extended detection scheme for enhancing the collection of scattered photons in nonlinear fluorescence microscopy by using a silicon photomultiplier array ahead of the front-apertures of objectives. We perform numerical simulations to demonstrate the enhanced fluorescence collection via extended epi-detection in the multi-photon fluorescence imaging of human skin and mouse brains through both craniotomy windows and intact skulls. For example, with red fluorescence emission at a depth of 600 μm in human skin, the increased collection can be as much as ~150% with a 10×, 0.6NA objective. We show that extended epi-detection is a generally applicable, feasible technique for use in nonlinear fluorescence microscopy to enhance signal detection.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE