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Journal of Zhejiang University SCIENCE A 2003 Vol.4 No.1 P.95~100

http://doi.org/10.1631/jzus.2003.0095


Quantitative assessment of the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis against the green peach aphid Myzus persicae


Author(s):  FENG Ming-guang, XU Qian

Affiliation(s):  Research Institute of Microbiology, College of Life Science, Zhejiang University,Hangzhou 310029, China

Corresponding email(s):   mgfeng@cls.zju.edu.cn

Key Words:  Zoophthora radicans, Myzus persicae, Infection rate, Dose dependence, Time-dose-mortality relationship, Conidia disinfection


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FENG Ming-guang, XU Qian. Quantitative assessment of the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis against the green peach aphid Myzus persicae[J]. Journal of Zhejiang University Science A, 2003, 4(1): 95~100.

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Abstract: 
A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis (Li) Humber, on the green peach aphid, myzus persicae (Sulzer). Firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial dosages (0.4-10.4 conidia/mm2) of Z. anhuiensis F97028, was used to yield an estimate of expected mortality probability at a given dosage. Secondly, bioassay 2 was conducted by simultaneously exposing six ≤4-day-old nymphal colonies to a shower of Z. anhuiensis conidia at each of four dosages (resulting from exposures of 0.3-8.0 min). Subsequently, the colonies were separately immersed in a 0.1% chlorothalonil solution for 0.5 min to disinfect all surviving conidia on the host integument from 1-12 h after exposure under temperature treatments of 15 and 20°C, respectively. The infection rate during a specific period from the end of the exposure to the immersion was then estimated as the ratio of the observed mortality over the expected mortality probability at a particular dosage. The results showed that the infection of M. persicae from Z. anhuiensis was highly rapid with little difference between aphid colonies maintained at 15 and 20°C before being immersed in the fungicidal solution after exposure. The first 6-hour period after exposure was most crucial to successful infection of the fungus with the infection rate greatly depending on conidial dosages. It took ≤1 h to infect>50% of the aphids at a dosage of >1.5 conida/mm2 and >90% at >50 conidia/mm2.

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