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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.8 P.936~940

http://doi.org/10.1631/jzus.2004.0936


Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method


Author(s):  JIANG Bing-bing, GAO Chang-you, HU Ling, SHEN Jia-cong

Affiliation(s):  Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   cygao@mail.hz.zj.cn

Key Words:  Co-precipitation, Nanoparticles, Bone morphogenetic proteins, Biopolymers


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JIANG Bing-bing, GAO Chang-you, HU Ling, SHEN Jia-cong. Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method[J]. Journal of Zhejiang University Science A, 2004, 5(8): 936~940.

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author="JIANG Bing-bing, GAO Chang-you, HU Ling, SHEN Jia-cong",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0936"
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%A JIANG Bing-bing
%A GAO Chang-you
%A HU Ling
%A SHEN Jia-cong
%J Journal of Zhejiang University SCIENCE A
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%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0936

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T1 - Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method
A1 - JIANG Bing-bing
A1 - GAO Chang-you
A1 - HU Ling
A1 - SHEN Jia-cong
J0 - Journal of Zhejiang University Science A
VL - 5
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SP - 936
EP - 940
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.0936


Abstract: 
A modified complex coacervation-co-precipitation method was used to prepare bone morphogenetic protein (BMP)-loaded nanospheres. Three natural polymers were used as packing materials to obtain nanoscale delivery device for BMP, in the presence of phosphatidylcholine functioning as stabilizer. Positively charged polysaccharide, N,N-diethylaminoethyl dextran (DEAE-dextran) tended to form stable, uniform and smaller size particles carrying BMP. Negatively charged bovine serum al-bumin (BSA) induced precipitation of the produced BMP particles due to its weak interaction with BMP molecules, although it produced nanosized BMP spheres. While collagen, a weakly positively charged protein shaped larger particles due to the strong interaction among themselves. A mechanism of co-precipitation process was also deduced to depict the formation of stable nanospheres.

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Reference

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