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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.4 P.280~287

http://doi.org/10.1631/jzus.2005.B0280


Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica


Author(s):  Zheng Min, Wu Yi-jun, Cai Wei-min, Weng Hong-lei, Liu Rong-hua

Affiliation(s):  Institute of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   minzheng@zju.edu.cn

Key Words:  Schistosomiasis japonica, Suppression subtractive hybridization, Differential expression


Zheng Min, Wu Yi-jun, Cai Wei-min, Weng Hong-lei, Liu Rong-hua. Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica[J]. Journal of Zhejiang University Science B, 2005, 6(4): 280~287.

@article{title="Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica",
author="Zheng Min, Wu Yi-jun, Cai Wei-min, Weng Hong-lei, Liu Rong-hua",
journal="Journal of Zhejiang University Science B",
volume="6",
number="4",
pages="280~287",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0280"
}

%0 Journal Article
%T Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica
%A Zheng Min
%A Wu Yi-jun
%A Cai Wei-min
%A Weng Hong-lei
%A Liu Rong-hua
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 4
%P 280~287
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0280

TY - JOUR
T1 - Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica
A1 - Zheng Min
A1 - Wu Yi-jun
A1 - Cai Wei-min
A1 - Weng Hong-lei
A1 - Liu Rong-hua
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 4
SP - 280
EP - 287
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0280


Abstract: 
To construct a hepatic stellate cells (HSCs) subtracted cDNA library to find differentially expressed genes in normal mice and mice infected with Schistosoma japonicum (S. japonicum). suppression subtractive hybridization (SSH) was used. The cDNA fragments of normal mouse were compared to those of schistosoma-infected mice to find differentially expressed genes. Then differentially expressed cDNA fragments were directly inserted into T/A cloning vector to set up the subtractive library. Amplification of the library was carried out with transformation of DH5α. The amplified library contained more than 400 positive bacterial clones, which were then hybridized with forward and backward subtracted probes for differential screening. One hundred positive bacterial clones were randomly selected for sequencing and BLAST analysis. Finally, virtual Northern Blot confirmed such differential expression. The subtracted cDNA library of differentially expressed genes of HSCs was constructed successfully, the library is efficient and lays foundation for screening and cloning new and specific genes of schistosomiasis.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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