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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.5 P.372-381


Persistence and intergenerational transmission of differentially expressed genes in the testes of intracytoplasmic sperm injection conceived mice

Author(s):  Li-ya Wang, Ning Wang, Fang Le, Lei Li, Le-jun Li, Xiao-zhen Liu, Ying-ming Zheng, Hang-ying Lou, Xiang-rong Xu, Xiao-ming Zhu, Yi-min Zhu, He-feng Huang, Fan Jin

Affiliation(s):  Key Laboratory of Reproductive Genetics (Zhejiang), Ministry of Education, and Centre of Reproductive Medicine, Womens Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China; more

Corresponding email(s):   jinfan@zju.edu.cn

Key Words:  Intracytoplasmic sperm injection, Testis, Intergenerational transmission

Li-ya Wang, Ning Wang, Fang Le, Lei Li, Le-jun Li, Xiao-zhen Liu, Ying-ming Zheng, Hang-ying Lou, Xiang-rong Xu, Xiao-ming Zhu, Yi-min Zhu, He-feng Huang, Fan Jin. Persistence and intergenerational transmission of differentially expressed genes in the testes of intracytoplasmic sperm injection conceived mice[J]. Journal of Zhejiang University Science B, 2013, 14(5): 372-381.

@article{title="Persistence and intergenerational transmission of differentially expressed genes in the testes of intracytoplasmic sperm injection conceived mice",
author="Li-ya Wang, Ning Wang, Fang Le, Lei Li, Le-jun Li, Xiao-zhen Liu, Ying-ming Zheng, Hang-ying Lou, Xiang-rong Xu, Xiao-ming Zhu, Yi-min Zhu, He-feng Huang, Fan Jin",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Persistence and intergenerational transmission of differentially expressed genes in the testes of intracytoplasmic sperm injection conceived mice
%A Li-ya Wang
%A Ning Wang
%A Fang Le
%A Lei Li
%A Le-jun Li
%A Xiao-zhen Liu
%A Ying-ming Zheng
%A Hang-ying Lou
%A Xiang-rong Xu
%A Xiao-ming Zhu
%A Yi-min Zhu
%A He-feng Huang
%A Fan Jin
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 5
%P 372-381
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200321

T1 - Persistence and intergenerational transmission of differentially expressed genes in the testes of intracytoplasmic sperm injection conceived mice
A1 - Li-ya Wang
A1 - Ning Wang
A1 - Fang Le
A1 - Lei Li
A1 - Le-jun Li
A1 - Xiao-zhen Liu
A1 - Ying-ming Zheng
A1 - Hang-ying Lou
A1 - Xiang-rong Xu
A1 - Xiao-ming Zhu
A1 - Yi-min Zhu
A1 - He-feng Huang
A1 - Fan Jin
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 5
SP - 372
EP - 381
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200321

intracytoplasmic sperm injection (ICSI) is commonly used to solve male infertility problems. Previous studies showed that early environmental exposure of an embryo may influence postnatal development. To detect whether ICSI operations affect the reproductive health of a male or his offspring, we established assisted reproductive technologies (ART) conceived mouse models, and analyzed gene expression profiles in the testes of both ICSI and naturally conceived (NC) newborn F1 mice using micro-array analysis. Among the differentially expressed genes, we focused on the expression of eight male reproduction-related genes. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was used to analyze the expression of these genes in the testes of both adult and old F1 generation mice and adult F2 generation mice. Our results showed that down-regulated and somatic cell-expressed genes in newborn mice retained their differential expression patterns in adult and old F1 generation individuals, implying the persistence and fetal origin of the alteration in the expression of these genes. The intergenerational transmission of differential gene expression was observed, but most changes tended to be reduced in adult F2 generations. Controlled ovarian hyperstimulation (COH) and in vitro fertilization (IVF) mice models were added to explore the precise factors contributing to the differences in ICSI offspring. The data demonstrated that superovulation, in vitro culture, and mechanical stimulation involved in ICSI had a cumulative effect on the differential expression of these male reproductive genes.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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