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On-line Access: 2012-04-06

Received: 2011-10-10

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.4 P.318-326


Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Author(s):  Tong-liang Bu, Yu-dong Jia, Jin-xing Lin, Yu-ling Mi, Cai-qiao Zhang

Affiliation(s):  Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   yulingmi@zju.edu.cn, cqzhang@zju.edu.cn

Key Words:  Quercetin, 3-Methyl-4-nitrophenol, Oxidative damage, Apoptosis, Germ cell, Testis

Tong-liang Bu, Yu-dong Jia, Jin-xing Lin, Yu-ling Mi, Cai-qiao Zhang. Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles[J]. Journal of Zhejiang University Science B, 2012, 13(11): 318-326.

@article{title="Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles",
author="Tong-liang Bu, Yu-dong Jia, Jin-xing Lin, Yu-ling Mi, Cai-qiao Zhang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles
%A Tong-liang Bu
%A Yu-dong Jia
%A Jin-xing Lin
%A Yu-ling Mi
%A Cai-qiao Zhang
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 4
%P 318-326
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer

T1 - Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles
A1 - Tong-liang Bu
A1 - Yu-dong Jia
A1 - Jin-xing Lin
A1 - Yu-ling Mi
A1 - Cai-qiao Zhang
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 4
SP - 318
EP - 326
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -

As a component of diesel exhaust particles, 3-Methyl-4-nitrophenol (4-nitro-m-cresol, PNMC) is also a metabolite of the insecticide fenitrothion and imposes hazardous effects on human health. In the present study, the alleviative effect of a common antioxidant flavonoid quercetin on mouse germ cells intoxicated by PNMC was investigated. Results showed that a single intraperitoneal injection of PNMC at 100 mg/kg induced severe testicular damage after one week. PNMC-treated mice showed a significant loss of germ cells (approximate 40% loss of round germ cells). PNMC caused an increase of hydroxyl radical and hydrogen peroxide production and lipid peroxidation, as well as a decrease in glutathione level, superoxide dismutase and glutathione peroxidase activities. Furthermore, treatment of PNMC increased expression of the pro-apoptotic protein Bax and decreased expression of the anti-apoptotic protein Bcl-XL in germ cells. In addition, testicular caspase-3 activity was significantly up-regulated and germ cell apoptosis was significantly increased in the PNMC-treated mice. In contrast, combined administration of quercetin at 75 mg/kg significantly attenuated PNMC-induced testicular toxicity. These results indicate that the antioxidant quercetin displays a remarkable protective effect on PNMC-induced oxidative damage in mouse testes and may represent an efficient supplement to attenuate reproductive toxicity by environmental toxicants to ensure healthy sperm production.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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