Full Text:   <1169>

Summary:  <392>

CLC number: R698+.2

On-line Access: 2015-09-05

Received: 2015-01-15

Revision Accepted: 2015-05-05

Crosschecked: 2015-08-10

Cited: 5

Clicked: 1891

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Bin Zhu

http://orcid.org/0000-0003-1771-9874

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.9 P.780-787

10.1631/jzus.B1500015


Protective effect of L-carnitine in cyclophosphamide-induced germ cell apoptosis


Author(s):  Bin Zhu, Yan-fei Zheng, Yue-ying Zhang, Yun-song Cao, Lei Zhang, Xin-gang Li, Teng Liu, Zhao-zhu Jiao, Qi Wang, Zhi-gang Zhao

Affiliation(s):  1Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China; more

Corresponding email(s):   1022zzg@sina.com

Key Words:  L-Carnitine, Infertility, Cyclophosphamide, LC3, Beclin-1


Bin Zhu, Yan-fei Zheng, Yue-ying Zhang, Yun-song Cao, Lei Zhang, Xin-gang Li, Teng Liu, Zhao-zhu Jiao, Qi Wang, Zhi-gang Zhao. Protective effect of L-carnitine in cyclophosphamide-induced germ cell apoptosis[J]. Journal of Zhejiang University Science B, 2015, 16(9): 780-787.

@article{title="Protective effect of L-carnitine in cyclophosphamide-induced germ cell apoptosis",
author="Bin Zhu, Yan-fei Zheng, Yue-ying Zhang, Yun-song Cao, Lei Zhang, Xin-gang Li, Teng Liu, Zhao-zhu Jiao, Qi Wang, Zhi-gang Zhao",
journal="Journal of Zhejiang University Science B",
volume="16",
number="9",
pages="780-787",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500015"
}

%0 Journal Article
%T Protective effect of L-carnitine in cyclophosphamide-induced germ cell apoptosis
%A Bin Zhu
%A Yan-fei Zheng
%A Yue-ying Zhang
%A Yun-song Cao
%A Lei Zhang
%A Xin-gang Li
%A Teng Liu
%A Zhao-zhu Jiao
%A Qi Wang
%A Zhi-gang Zhao
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 9
%P 780-787
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500015

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T1 - Protective effect of L-carnitine in cyclophosphamide-induced germ cell apoptosis
A1 - Bin Zhu
A1 - Yan-fei Zheng
A1 - Yue-ying Zhang
A1 - Yun-song Cao
A1 - Lei Zhang
A1 - Xin-gang Li
A1 - Teng Liu
A1 - Zhao-zhu Jiao
A1 - Qi Wang
A1 - Zhi-gang Zhao
J0 - Journal of Zhejiang University Science B
VL - 16
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SP - 780
EP - 787
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500015


Abstract: 
cyclophosphamide (CP) is a widely used anti-cancer agent; however, it can also induce serious male infertility. There are currently no effective drugs to alleviate this side-effect. L-Carnitine has been used to treat male infertility, but whether it can be used to protect against CP-induced male infertility is still unclear. This study aims to explore the effect and mechanism of L-Carnitine in male infertility induced by CP. CP was used to establish an animal model. After three weeks of treatment, rats were sacrificed and testis and serum were harvested for further evaluation. Testosterone and estrogen levels were measured by enzyme-linked immunosorbent assay (ELISA). Testicular injury was examined by hematoxylin and eosin (H & E) staining, and germ-cell apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). The expression of LC3 and beclin-1 was examined by immunohistochemistry, Western blot, and real-time polymerase chain reaction (PCR), respectively. Compared with the CP group, L-Carnitine significantly increases sperm motility, viability, and testosterone level (P<0.05). Western blot and real-time PCR results showed that L-Carnitine treatment can significantly up-regulate the LC3-II and beclin-1 expression in the CP+L-Carnitine group when compared with the control group (P<0.05). In addition, TUNEL-positive cells were also more numerous in the CP group; however, L-Carnitine can effectively retard cell apoptosis in the CP+ L-Carnitine group. In conclusion, L-Carnitine contributes to the inhibition of cell apoptosis and the modulation of autophagy in protecting CP-induced testicular injury. These results suggest the applicability of L-Carnitine in the treatment of male infertility.

左卡尼汀对环磷酰胺诱导的生精细胞凋亡的疗效机制研究

目的:环境因素及抗癌药物的使用会导致生精细胞出现凋亡,从而引起男性不育。目前尚缺乏疗效明确的治疗药物。左卡尼汀被用来治疗男性不育取得了一定的疗效。本研究拟通过环磷酰胺诱导的大鼠不育模型,研究左卡尼汀对生精细胞的保护作用。
创新点:首次观察了左卡尼汀改善化疗药物环磷酰胺所导致的生殖功能障碍的疗效。
方法:采用环磷酰胺诱导大鼠不育模型成功后,给予左卡尼汀治疗15天,然后处死全部大鼠。检测血清中的睾酮和雌激素含量;采用全自动精子分析仪检测附睾组织中精子的数量和质量;采用苏木精-伊红(H & E)染色观察睾丸组织的病理损伤;通过末端脱氧核苷酸转移酶介导的 d UTP缺口标记技术(TUNEL)的染色观察睾丸组织中生精细胞的凋亡;采用免疫组织化学法观察睾丸组织中LC3和Beclin-1的表达;采用蛋白质免疫印迹分析(Western blot)和多聚酶链式反应(PCR)分别检测组织中LC3和Beclin-1的表达。
结论:左卡尼汀可以通过增加细胞自噬,抑制生精细胞凋亡,提高生精细胞的质量和数量,进而降低环磷酰胺引起的睾丸组织的损伤。

关键词:左卡尼汀;男性不育;环磷酰胺;LC3;Beclin-1

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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