CLC number: Q25
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2015-11-24
Cited: 4
Clicked: 4937
Hai-sheng Xu, Sun-jian Lyu, Jie-hao Xu, Bin-jie Lu, Jing Zhao, Song Li, Yi-qun Li, Yu-yin Chen. Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis[J]. Journal of Zhejiang University Science B, 2015, 16(12): 971-979.
@article{title="Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis",
author="Hai-sheng Xu, Sun-jian Lyu, Jie-hao Xu, Bin-jie Lu, Jing Zhao, Song Li, Yi-qun Li, Yu-yin Chen",
journal="Journal of Zhejiang University Science B",
volume="16",
number="12",
pages="971-979",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500098"
}
%0 Journal Article
%T Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis
%A Hai-sheng Xu
%A Sun-jian Lyu
%A Jie-hao Xu
%A Bin-jie Lu
%A Jing Zhao
%A Song Li
%A Yi-qun Li
%A Yu-yin Chen
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 12
%P 971-979
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500098
TY - JOUR
T1 - Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis
A1 - Hai-sheng Xu
A1 - Sun-jian Lyu
A1 - Jie-hao Xu
A1 - Bin-jie Lu
A1 - Jing Zhao
A1 - Song Li
A1 - Yi-qun Li
A1 - Yu-yin Chen
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 12
SP - 971
EP - 979
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500098
Abstract: In the present study, we investigated the possible toxicity mechanism of lipopolysaccharide (LPS) extracted from Gram-negative bacteria in Eriocheir sinensis hemocytes. Apoptotic hemocytes and reactive oxygen species (ROS) production induced by the LPS were monitored by the combination of flow cytometry and microscope observation. It was shown that LPS induced serious damage on the DNA and morphological changes in hemocytes, including cell shrinkage, fracture of nucleus membrane, margination, condensation and fragmentation of chromatin, and formation of apoptotic bodies indicating obvious hemocyte apoptosis. As compared with the control group, the apoptotic cell ratio increased to 30.61% and 39.01% after 1-h exposure and 57.72% and 75.01% after 2-h exposure to 1 and 10 μg/ml LPS, respectively (P<0.05). Significant outburst of ROS production was observed in LPS-treated hemocytes with approximately 176.6% of relative dichlorofluorescein mean fluorescence at 1-h exposure, followed by a drastic decline (P<0.05). These results indicated that LPS would induce oxidative stress on hemocytes from E. sinensis and cause ROS burst, DNA damage, and subsequently apoptosis. The process of ROS-mediated apoptosis might be one of the potential toxicity mechanisms of LPS on crustacean hemocytes.
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