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Received: 2005-03-21

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.8 P.817~824

http://doi.org/10.1631/jzus.2005.B0817


Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea


Author(s):  WANG Jiao-yu, LIU Xiao-hong, LU Jian-ping, LIN Fu-cheng

Affiliation(s):  Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   fuchenglin@zju.edu.cn

Key Words:  Magnaporthe grisea, MGTA1 gene, Zn(II)2Cys6


WANG Jiao-yu, LIU Xiao-hong, LU Jian-ping, LIN Fu-cheng. Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea[J]. Journal of Zhejiang University Science B, 2005, 6(8): 817~824.

@article{title="Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea",
author="WANG Jiao-yu, LIU Xiao-hong, LU Jian-ping, LIN Fu-cheng",
journal="Journal of Zhejiang University Science B",
volume="6",
number="8",
pages="817~824",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0817"
}

%0 Journal Article
%T Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea
%A WANG Jiao-yu
%A LIU Xiao-hong
%A LU Jian-ping
%A LIN Fu-cheng
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 8
%P 817~824
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0817

TY - JOUR
T1 - Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea
A1 - WANG Jiao-yu
A1 - LIU Xiao-hong
A1 - LU Jian-ping
A1 - LIN Fu-cheng
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 8
SP - 817
EP - 824
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0817


Abstract: 
MGTA1, a putative fungal zn(II)2Cys6 transcriptional activator-encoding gene, was isolated from rice blast pathogen Magnaporthe grisea, which is homologous to CLTA1 from Colletotrichum lindemuthianum with 51% identity at protein level. MGTA1 cassette contains a 2370 bp open reading frame, consisting of 6 exons, coding a 790 amino acid peptide. MGTA1 gene exists as a single copy in genomes of 7 strains of M. grisea, and is expressed in tip hyphae, conidia, and mature appressoria of strain Guy11.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Aist, J.R., Bushnell, W.R., 1991. Invasion of Plants by Powdery Mildew Fungi, and Cellular Mechanisms of Resistance. In: Cole, G.T., Hoch, H.C. (Eds.), The Fungal Spore and Disease Initiation in Plants and Animals. Plenum Press, New York, p.321-345.

[2] Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucl. Acids Res., 25:3389-3402.

[3] Bailey, J.A., Jeger, M.J., 1992. Colletotrichum: Biology, Pathology and Control. CAB International, Wallingford.

[4] Balhadere, P.V., Talbot, N.J., 2001. PDE1 encodes a P-type ATPase involved in appressorium-mediated plant infection by the rice blast fungus Magnaporthe grisea. Plant Cell, 13:1987-2004.

[5] Bourett, T.M., Howard, R.J., 1990. In vitro development of penetration structures in the rice blast fungus Magnaporthe grisea. Can. J. Bot., 69:329-342.

[6] Bourett, T.M., Howard, R.J., 1992. Actin in penetration pegs of the fungal rice blast pathogen, Magnaporthe grisea. Protoplasma, 168:20-26.

[7] Caroll, A.M., Sweigard, J.A., Valent, B., 1994. Improved vectors for selecting resistance to hygromycin. Fungal Genet. Newslett., 41:22.

[8] Dufresne, M., Perfect, S., Pellier, A.L., Bailey, J.A., Langin, T., 2000. A GAL4-like protein is involved in the switch between biotrophic and necrotrophic phases of the infection process of Colletotrichum lindemuthianum on common bean. Plant Cell, 12:1579-1590.

[9] Hamer, J.E., Howard, R.J., Chumley, F.G., Valent, B., 1988. A mechanism for surface attachment in spores of a plant pathogenic fungus. Science, 239:288-290.

[10] Heath, M.C., Valent, B., Howard, R.J., Chumley, F.G., 1990a. Correlations between cytologically detected plant-funga1 interactions and pathogenicity of Magnaporthe grisea toward weeping lovegrass. Phytopathology, 80:1382-1386.

[11] Heath, M.C., Valent, B., Howard, R.J., Chumley, F.G., 1990b. Interactions of two strains of Magnaporthe grisea with rice, goosegrass and weeping lovegrass. Can. J. Bot., 68:1627-1637.

[12] Higgins, D.G., Bleasby, A.J., Fuchs, R., 1992. CLUSTAL V: Improved software for multiple sequence alignment. Comput. Appl. Biosci., 8:189-191.

[13] Howard, R.J., Ferrari, M.A., 1989. Role of melanin in appressorium function. Exp. Mycol., 13:403-418.

[14] Howard, R.J., Bourett, T.M., Ferrari, M.A., 1991. Infection by Magnaporthe Grisea: An in vitro Analysis. In: Mendgen, K., Lesemann, D.E. (Eds.), Electron Microscopy of Plant Pathogens. Springer-Verlag, Berlin, p.251-264.

[15] Kunoh, H., Aist, J.R., Israel, H.W., 1979. Primary germ tubes and host cells penetrations from appressoria of Erysiphe graminis hordei. Ann. Phytopathol. Soc. Japan, 45:326-332.

[16] Lu, J.P., Liu, T.B., Yu, X.Y., Lin, F.C., 2005. Representative appressorium stage cDNA library of Magnaporthe grisea. J. Zhejiang Univ. Sci., 6B(2):132-136.

[17] O’Connell, R.J., Bailey, J.A., Richmond, D.V., 1985. Cytology and physiology of infection of Phaseolus vulgaris by Colletotrichum lindemuthianum. Physiol. Plant Pathol., 27:75-98

[18] Ou, S.H., 1985. Rice Diseases. Kew, Surrey, Commonwealth Mycological Institute, CABI, p.109-201.

[19] Perfect, S.E., Hughes, H.B., O’Connell, R.J., Green, J.R., 1999. Colletotrichum: A model genus for studies on pathology and fungal-plant interactions. Fungal Genetics and Biology, 27:186-198.

[20] Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

[21] Staben, C., Jensen, B., Singer, M., Pollock, J., Schechtman, M., Kinsey, J., Selker, E., 1989. Use of a bacterial Hygromycin B resistance gene as a dominant selectable marker in Neurospora crassa transformation. Fungal Genet. Newsl., 36:79-81.

[22] Talbot, N.J., 1995. Having a blast: Exploring the pathogenicity of Magnaporthe grisea. Trends Microbiol., 3:9-16.

[23] Talbot, N.J., Ebbole, D.J., Hamer, J.E., 1993. Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell, 5:1575-1590.

[24] Todd, R.B., Andrianopoulos, A., 1997. Evolution of a fungal regulatory gene family: The Zn(II)2Cys6 binuclear cluster DNA binding motif. Fungal Genetics and Biology, 21:388-405.

[25] Xu, J.R., Hamer, J.E., 1996. MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe grisea. Genes Dev., 10:2696-2706.

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