Full Text:   <2593>

CLC number: R657.3

On-line Access: 

Received: 2005-06-10

Revision Accepted: 2005-09-27

Crosschecked: 0000-00-00

Cited: 2

Clicked: 5849

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.12 P.1188-1194

http://doi.org/10.1631/jzus.2005.B1188


Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation


Author(s):  XIE Hai-yang, HUANG Dong-sheng, JIA Chang-ku, ZHENG Shu-sen

Affiliation(s):  Department of Hepatobiliary Pancreatic Surgery, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   Zhengss@mail.hz.zj.cn

Key Words:  Liver transplantation, Rejection, Bone marrow infusion, Chimerism


XIE Hai-yang, HUANG Dong-sheng, JIA Chang-ku, ZHENG Shu-sen. Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation[J]. Journal of Zhejiang University Science B, 2005, 6(12): 1188-1194.

@article{title="Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation",
author="XIE Hai-yang, HUANG Dong-sheng, JIA Chang-ku, ZHENG Shu-sen",
journal="Journal of Zhejiang University Science B",
volume="6",
number="12",
pages="1188-1194",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B1188"
}

%0 Journal Article
%T Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation
%A XIE Hai-yang
%A HUANG Dong-sheng
%A JIA Chang-ku
%A ZHENG Shu-sen
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 12
%P 1188-1194
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B1188

TY - JOUR
T1 - Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation
A1 - XIE Hai-yang
A1 - HUANG Dong-sheng
A1 - JIA Chang-ku
A1 - ZHENG Shu-sen
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 12
SP - 1188
EP - 1194
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B1188


Abstract: 
Objective: To study the effect and implication of nonmyeloablative donor specific bone marrow (DSBM) infusion on the immunoreaction of liver allotransplantation. Methods: Orthotopic liver transplantation model was used in this study. Groups were set as follows: Group I, syngeneic control (Wistar-to-Wistar); Group II, acute rejection (SD-to-Wistar); Group III, acute rejection treated with cyclosporine A (CsA) by intramuscular injection (SD-to-Wistar+CsA); Group IV, bone marrow infusion at 7 d pretransplantation followed by short-term CsA treatment (SD-to-Wistar+DSBM); Another group of short-term CsA treatment preoperatively without bone marrow infusion was also set as control. General characteristics and survival time were observed. Histological grades of rejection were determined by pathological examination. IL-2 and IFN-γ level in peripheral blood and donor liver were detected respectively by Enzyme-Linked Immuno-Sorbent Assay (ELISA) and Western blot. chimerism of donor cells was measured by PCR for a male-specific marker (Y-chromosome-specific sequence, Sry). Results: No signs of rejection were found in Group I. Acute rejection occurred in both Group II and the short-term CsA treated group. All the recipients died at (9~15) d posttransplantation with a median survival time of (10.7±0.5) d and (11.2±2.4) d, respectively. Only mild rejection could be seen in Group III. In Group IV, 4 out of 6 recipients had long-term survival (>100 d), the histological grade of rejection was significantly lower than that of Group II, so did the expression level of IL-2 and IFN-γ in both peripheral blood and grafted liver. Y-chromosome-specific sequence (Sry) of male SD rats could be detected in the bone marrow, spleen and thymus of female recipients at 15 d after bone marrow infusion. Conclusion: Mild preconditioning nonmyeloablative donor specific bone marrow infusion can enhance chimerism formation in recipients, alleviate the rejection of liver allotransplantation and prolong survival of liver allotransplantation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Alberto, S.F., Terry, B.S., 2004. Immunological tolerance and liver transplantation. Journal of Hepatology, 41(5):698-705.

[2] Beschorner, W.E., Yao, X., Divic, J., 1995. Recruitment of semiallogeneic dendritic cells to the thymus during post-cyclosporine thymic regeneration. Transplantation, 60(11):1326-1330.

[3] Gao, E.K., Lo, D., Cheney, R., Kanagawa, O., Sprent, J., 1988. Abnormal differentiation of thymocytes in mice treated with cyclosporine A. Nature, 336(6195):176-179.

[4] Hale, D.A., Gottschalk, R., Umemura, A., Maki, T., Monaco, A.P., 2000. Establishment of stable multilineage hematopoietic chimerism and donor-specific tolerance without irradiation. Transplantation, 69(7):1242-1251.

[5] Jenkins, M.K., Schwartz, R.H., Pardoll, D.M., 1988. Effects of cyclosporine A on T cell development and clonal deletion. Science, 241(4873):1655-1658.

[6] Jia, C.K., Zheng, S.S., Zhang, A.B., 2003. Intrathymic inoculation of donor liver specific antigen alleviates rejection of liver allotransplantation. Journal of Zhejiang University Science, 4(4):485-490.

[7] Joseph, S., David, W.R., 2000. Molecular Cloning: A Laboratory Manual on the Web, 3rd Ed. Cold Spring Harbor Laboratory (CSHL) Press, New York, p.127-128.

[8] Kemnitz, J., Ringe, B., Cohnert, T.R., Gubernatis, G., Choritz, H., Georgii, A., 1989. Bile duct injury as a part of diagnostic criteria for liver allograft rejection. Hum. Pathol., 20(2):132-143.

[9] Kita, Y., Li, X.K., Ohba, M., Funeshima, N., Enosawa, S., Tamura, A., Suzuki, K., Amemiya, H., Hayashi, S., Kazui, T., Suzuki, S., 1999. Prolonged cardiac allograft survival in rats systemically injected adenoviral vectors containing CTLA4Ig-gene. Transplantation, 68(6):758-766.

[10] Kobayashi, E., Kamada, N., Goto, S., Miyata, M., 1993. Protocol for the technique of orthotopic liver transplantation in the rat. Microsurgery, 14(8):541-546.

[11] Lee, W.C., Jeng, L.B., Chiang, Y.J., Wang, H.C., Huang, C.C., 2000. Dendritic cell progenitors prolong allograft survival through T-helper 2 deviation of the Th1/Th2 paradigm. Transplant Proc., 32(7):2076-2077.

[12] Li, S., Thanikachalam, M., Pang, M., Kawaharada, N., Aitouche, A., Pham, S.M., 2001. A clinically relevant CTLA4-Ig-based regimen induces chimerism and tolerance to heart grafts. Ann. Thorac. Surg., 72(4):1306-1310.

[13] Liu, J., 1993. FK506 and cyclosporine, molecular probes for studying intracellular signal transduction. Immunol. Today, 14(6):290-295.

[14] Manilay, J.O., Pearson, D.A., Sergio, J.J., Swenson, K.G., Sykes, M., 1998. Intrathymic deletion of alloreactive T cells in mixed bone marrow chimeras prepared with a nonmyeloablative conditioning regimen. Transplantation, 66(1):96-102.

[15] Petersen, B.E., Bowen, W.C., Patrene, K.D., Mars, W.M., Sullivan, A.K., Murase, N., Boggs, S.S., Greenberger, J.S., Goff, J.P., 1999. Bone marrow as a potential source of hepatic oval cells. Science, 284(5417):1168-1170.

[16] Raimondo, M.L., Burroughs, A.K., 2002. Single-agent immunosuppression after liver transplantation: what is possible? Drugs, 62(11):1587-1597.

[17] Shirasugi, N., Adams, A.B., Durham, M.M., Lukacher, A.E., Xu, H., Rees, P., Cowan, S.R., Williams, M.A., Pearson, T.C., Larsen, C.P., 2002. Prevention of chronic rejection in murine cardiac allografts: a comparison of chimerism- and nonchimerism-inducing costimulation blockade-based tolerance induction regimens. J. Immunol., 169(5):2677-2684.

[18] Theise, N.D., Badve, S., Saxena, R., Henegariu, O., Sell, S., Crawford, J.M., Krause, D.S., 2000. Derivation of hepatocytes from bone marrow cells in mice after radiation-induced myeloahlation. Hepatology, 31(1):235-240.

[19] Urdahl, K.B., Pardoll, D.M., Jenkins, M.K., 1994. Cyclosporin A inhibits positive selection and delays negative selection in αβ TCR transgenic mice. J. Immunol., 152(6):2853-2859.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE