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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.7 P.521-531


In-situ nitrogen removal from the eutrophic water by microbial-plant integrated system

Author(s):  CHANG Hui-qing, YANG Xiao-e, FANG Yun-ying, PU Pei-min, LI Zheng-kui, RENGEL Zed

Affiliation(s):  Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, School of Natural Resources and Environmental Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   xyang581@yahoo.com

Key Words:  Nitrogen, Immobilized nitrogen-cycling bacteria (INCB), Eutrophication, Eichhornia crassipes, Elodea nuttallii

CHANG Hui-qing, YANG Xiao-e, FANG Yun-ying, PU Pei-min, LI Zheng-kui, RENGEL Zed. In-situ nitrogen removal from the eutrophic water by microbial-plant integrated system[J]. Journal of Zhejiang University Science B, 2006, 7(7): 521-531.

@article{title="In-situ nitrogen removal from the eutrophic water by microbial-plant integrated system",
author="CHANG Hui-qing, YANG Xiao-e, FANG Yun-ying, PU Pei-min, LI Zheng-kui, RENGEL Zed",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T In-situ nitrogen removal from the eutrophic water by microbial-plant integrated system
%A CHANG Hui-qing
%A YANG Xiao-e
%A FANG Yun-ying
%A PU Pei-min
%A LI Zheng-kui
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 7
%P 521-531
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0521

T1 - In-situ nitrogen removal from the eutrophic water by microbial-plant integrated system
A1 - CHANG Hui-qing
A1 - YANG Xiao-e
A1 - FANG Yun-ying
A1 - PU Pei-min
A1 - LI Zheng-kui
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 7
SP - 521
EP - 531
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0521

Objective: This study was to assess the influence of interaction of combination of immobilized nitrogen cycling bacteria (INCB) with aquatic macrophytes on nitrogen removal from the eutrophic waterbody, and to get insight into different mechanisms involved in nitrogen removal. Methods: The aquatic macrophytes used include Eichhornia crassipes (summer-autumn floating macrophyte), Elodea nuttallii (winter-growing submerged macrophyte), and nitrogen cycling bacteria including ammonifying, nitrosating, nitrifying and denitrifying bacteria isolated from Taihu Lake. The immobilization carriers materials were made from hydrophilic monomers 2-hydroxyethyl acrylate (HEA) and hydrophobic 2-hydroxyethyl methylacrylate (HEMA). Two experiments were conducted to evaluate the roles of macrophytes combined with INCB on nitrogen removal from eutrophic water during different seasons. Results: Eichhornia crassipes and Elodea nuttallii had different potentials in purification of eutrophic water. Floating macrophyte+bacteria (INCB) performed best in improving water quality (during the first experiment) and decreased total nitrogen (TN) by 70.2%, nitrite and ammonium by 92.2% and 50.9%, respectively, during the experimental period, when water transparency increased from 0.5 m to 1.8 m. When INCB was inoculated into the floating macrophyte system, the populations of nitrosating, nitrifying, and denitrifying bacteria increased by 1 to 2 orders of magnitude compared to the un-inoculated treatments, but ammonifying bacteria showed no obvious difference between different treatments. Lower values of chlorophyll a, CODMn, and pH were found in the microbial-plant integrated system, as compared to the control. Highest reduction in N was noted during the treatment with submerged macrophyte+INCB, being 26.1% for TN, 85.2% for nitrite, and 85.2% for ammonium at the end of 2nd experiment. And in the treatment, the populations of ammonifying, nitrosating, nitrifying, and denitrifying bacteria increased by 1 to 3 orders of magnitude, as compared to the un-inoculated treatments. Similar to the first experiment, higher water transparency and lower values of chlorophyll a, CODMn and pH were observed in the plant+ INCB integrated system, as compared to other treatments. These results indicated that plant-microbe interaction showed beneficial effects on N removal from the eutrophic waterbody.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Brix, H., 1994. Functions of macrophytes in constructed wetlands. Wat. Sci. Tech., 29(4):71-78.

[2] Cao, G.M., Zhao, Q.X., Sun, X.B., Zhang, T., 2002. Characterization of nitrifying and denitrifying bacteria coimmobilized in PVA and kinetics model of biological nitrogen removal by coimmobilized cells. Enzyme Microb. Technol., 30(1):49-55.

[3] Chang, H.Q., Yang, X.E., Fang, Y.Y., 2005. Effects on nutrient of eutrophicated water by Elodea nuttallii and immobilized bacteria. Journal of Soil and Water Conservation, 19(3):114-117.

[4] dos Santos, V.M.P.M., Bruijnse, M., Tramper, J., Wijffels, R.H., 1996. The magic-beads concept: an integrated approach to nitrogen removal with co-immobilized micro-organisms. Appl. Microbiol. Biotechnol., 45(4):447-453.

[5] Eighmy, T.T., Bishop, P.L., 1989. Distribution and role of bacterial nitrifying populations in nitrogen removal in aquatic treatment systems. Wat. Res., 23(8):947-955.

[6] Herbert, R.A., 1999. Nitrogen cycling in coastal marine ecosystems. FEMS Microbiol. Rev., 23(5):563-590.

[7] Jeppesen, E., Kristensen, P., Jensen, J.P., Søndergaard, M., Mortensen, E., Lauridsen, T., 1991. Recovery resilience following a reduction in external phosphorus loading of shallow, eutrophic Danish lakes: duration, regulating factors and methods for overcoming resilience. Mem. Ist. Ital. Idrobiol., 48(1):127-148.

[8] Kim, Y., Giokas, D.L., Lee, J.W., Paraskevas, P.A., 2006. Potential of natural treatment systems for the reclamation of domestic sewage in irrigated agriculture. Desalination, 189(1-3):229-242.

[9] Körner, S., 1999. Nitrifying and denitrifying bacteria in epiphytic communities of submerged macrophytes in a treated sewage channel. Acta Hydrochim. Hydrobiol., 27(1):27-31.

[10] Körner, S., Vermaat, J.E., 1998. The relative importance of Lemna gibba L., bacteria and algae for the nitrogen and phosphorus removal in duckweed-covered domestic wastewater. Water Research, 32(12):3651-3661.

[11] Kufel, L., Ozimek, T., 1994. Can Chara control phosphorus cycling in Lake Luknajno (Poland). Hydrobiologia, 275/276(1):277-283.

[12] Lauridsen, T.L., Jeppesen, E., Søndergaard, M., 1994. Colonization and succession of submerged macrophytes in shallow Lake Vaeng during the first 5 years following fish manipulation. Hydrobiologia, 275/276(1):233-242.

[13] Li, W.C., 1997. Construction and purification efficiency test of an ever-green aquatic vegetation in an eutrophic lake. China Enviromental Science, 17(1):53-57 (in Chinese).

[14] Li, Z.K., Pu, P.M., 2000. The dynamic mode of INCB purifying the polluted lake by nitrogen compound in the autumn-winter seasons. Lake Science, 1-2(4):321-326 (in Chinese).

[15] Li, Z.K., Pu, P.M., 2001a. The dynamic mode of INCB purifying the polluted lake by nitrogen compound in the autumn-winter seasons. Lake Science, 12(4):321-326 (in Chinese).

[16] Li, Z.K., Pu, P.M., 2001b. The technics immobilized nitration bacteria removal ammonium in low temperature radiation. Jiangsu Agriculture, 16(2):115-117 (in Chinese).

[17] Li, F.D., Yu, Z.N., He, S.J., 1996. Experimental Techniques of Agricultural Microorganism. Chinese Agriculture Press, Beijing, China, p.44-50 (in Chinese).

[18] Livingston, R.J., McGlynn, S.E., Niu, X., 1998. Factors controlling seagrass growth in a gulf coastal system: water quality and light. Aquat. Bot., 60(2):135-159.

[19] Matulewich, V.A., Finstein, M.S., 1978. Distribution of autotrophic nitrifying bacteria in a polluted river (the Passaic). Applied and Environmental Microbiology, 35(1):67-71.

[20] Neundorfer, J.V., Kemp, W.M., 1993. Nitrogen versus phosphorus enrichment of brackish waters: responses of the submersed plant Potamogeton perfoliatus and its associated algal community. Mar. Ecol. Prog. Ser., 94:71-82.

[21] Northup, R.R., Zengshou, Y.U., Randy, A., 1995. Polyphenol control of nitrogen release from pine litter. Nature, 377(21):227-229.

[22] Ntengwe, F.W., 2005. An overview of industrial wastewater treatment and analysis as means of preventing pollution of surface and underground water bodies―the case of Nkana Mine in Zambia. Physics and Chemistry of the Earth, 30(11-16):726-734.

[23] Rattray, M.R., Howard-Williams, C., Brown, J.M.A., 1991. Sediment and water as sources of nitrogen and phosphorus for submerged rooted aquatic macrophytes. Aquat. Bot., 40(3):225-237.

[24] Sakairi, M.A.C., Kimiaki Yasuda, K., Matsumura, M., 1996. Nitrogen removal in seawater using nitrifying and denitrifying bacteria immobilized in porous cellulose carrier. Water Science and Technology, 34(7-8):267-274.

[25] Seddon, S., Connolly, R.S., Edyvane, K.S., 2000. Large-scale seagrass die back in northern Spencer Gulf, South Australia. Aquat. Bot., 66(4):297-310.

[26] Smith, W.H., Bormann, F.H., Likens, G.E., 1968. Response of chemoautotrophic nitrifiers to forest cutting. Soil Sci., 106(6):471-473.

[27] Sooknah, R.D., Wilkie, A.C., 2004. Nutrient removal by floating aquatic macrophytes cultured in anaerobically digested flushed dairy manure wastewater. Ecological Engineering, 22(1):27-42.

[28] Takizawa, S., Aravinthan, V., Fujita, K., 1996. Nitrogen removal from domestic wastewater using immobilized bacteria. Water Science and Technology, 34(1-2):431-440.

[29] van Donk, E., van de Bund, W.J., 2002. Impact of submerged macrophytes including charophytes on phyto- and zooplankton communities: allelopathy versus other mechanisms. Aquat. Bot., 72(3-4):261-274.

[30] Wei, F.S., Qi, W.Q., Bi, T., 2002. Inspectorial and Analytical Methods of Water and Wastewater Transaction. Chinese Environmental Sciences Press, Beijing, p.1254-1284 (in Chinese).

[31] Wu, Z.B., 2003. The effect of submerge plant recovery on the nitrogen and phosphorus of the eutrophic water. Chinese Journal of Applied Ecology, 14(8):1351-1353 (in Chinese).

[32] Yang, X.E., Tong, C.H., Pu, P.M., 2003. The effects and mechanism of aquatic plant control the nutrient release from the lake sediment. Agriculture Environment Science Journal, 22(6):673-676 (in Chinese).

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