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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.8 P.648~653

10.1631/jzus.2006.B0648


Effects of angiotensin II on connexin 43 of VSMCs in arteriosclerosis


Author(s):  CAI Wei, RUAN Li-ming, WANG Yi-na, CHEN Jun-zhu

Affiliation(s):  Department of Internal Medicine, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   weic236@sohu.com

Key Words:  Atherosclerosis, Connexin, mRNA, Losartan, Ramipril


CAI Wei, RUAN Li-ming, WANG Yi-na, CHEN Jun-zhu. Effects of angiotensin II on connexin 43 of VSMCs in arteriosclerosis[J]. Journal of Zhejiang University Science B, 2006, 7(8): 648~653.

@article{title="Effects of angiotensin II on connexin 43 of VSMCs in arteriosclerosis",
author="CAI Wei, RUAN Li-ming, WANG Yi-na, CHEN Jun-zhu",
journal="Journal of Zhejiang University Science B",
volume="7",
number="8",
pages="648~653",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0648"
}

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%T Effects of angiotensin II on connexin 43 of VSMCs in arteriosclerosis
%A CAI Wei
%A RUAN Li-ming
%A WANG Yi-na
%A CHEN Jun-zhu
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 8
%P 648~653
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0648

TY - JOUR
T1 - Effects of angiotensin II on connexin 43 of VSMCs in arteriosclerosis
A1 - CAI Wei
A1 - RUAN Li-ming
A1 - WANG Yi-na
A1 - CHEN Jun-zhu
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 8
SP - 648
EP - 653
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0648


Abstract: 
Objective: To observe the effect of angiotensin II (Ang II) on expression of gap junction channel protein connexin 43 (Cx43) in the proliferation process of vascular smooth muscle cells (VSMCs) during the early stage of arteriosclerosis. Methods: Thirty-two adult male rabbits were randomly divided into 4 groups. Rabbits in Group A were fed common diet while others in Groups B, C, and D were fed high-cholesterol diet. losartan (10 mg/(kg∙d)) and ramipril (0.5 mg/(kg∙d)) were added in the diet of Groups C and D, respectively. The animals were sacrificed after 8 weeks and abdominal aortas were removed and dissected. The expression of Cx43 was assayed using RT-PCR and Western Blotting analysis. Results: Cx43 was increased markedly in both protein and mRNA level in Groups B, C, and D fed high-cholesterol diet compared with that in control group (P<0.01). Cx43 level in losartan or ramipril treated groups was higher than that in control group (P<0.01, P<0.05), but lower than that in high-cholesterol diet groups (P<0.05, P<0.01). Conclusion: Cx43 level was upregulated in VSMCs during early atherosclerosis. losartan and ramipril can inhibit the expression of Cx43.

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