Full Text:   <1690>

CLC number: X122; X172

On-line Access: 

Received: 2006-07-03

Revision Accepted: 2006-12-05

Crosschecked: 0000-00-00

Cited: 6

Clicked: 3062

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2007 Vol.8 No.11 P.1846~1852

http://doi.org/10.1631/jzus.2007.A1846


Effects of N source and nitrification pretreatment on growth of Arthrospira platensis in human urine


Author(s):  FENG Dao-lun, WU Zu-cheng, WANG Da-hui

Affiliation(s):  Department of Environmental Science and Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   fengdaolun2000@yahoo.com.cn, wuzc@zju.edu.cn

Key Words:  Arthrospira platensis, Human urine, N source, Nitrification, Bioregenerative life support system (BLSS)


FENG Dao-lun, WU Zu-cheng, WANG Da-hui. Effects of N source and nitrification pretreatment on growth of Arthrospira platensis in human urine[J]. Journal of Zhejiang University Science A, 2007, 8(11): 1846~1852.

@article{title="Effects of N source and nitrification pretreatment on growth of Arthrospira platensis in human urine",
author="FENG Dao-lun, WU Zu-cheng, WANG Da-hui",
journal="Journal of Zhejiang University Science A",
volume="8",
number="11",
pages="1846~1852",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.A1846"
}

%0 Journal Article
%T Effects of N source and nitrification pretreatment on growth of Arthrospira platensis in human urine
%A FENG Dao-lun
%A WU Zu-cheng
%A WANG Da-hui
%J Journal of Zhejiang University SCIENCE A
%V 8
%N 11
%P 1846~1852
%@ 1673-565X
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.A1846

TY - JOUR
T1 - Effects of N source and nitrification pretreatment on growth of Arthrospira platensis in human urine
A1 - FENG Dao-lun
A1 - WU Zu-cheng
A1 - WANG Da-hui
J0 - Journal of Zhejiang University Science A
VL - 8
IS - 11
SP - 1846
EP - 1852
%@ 1673-565X
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.A1846


Abstract: 
Culture of Arthrospira platensis (Spirulina platensis) in human urine was investigated to get valuable biomass. NO3-N was the proper n source, in comparison with other n source, including urea, NH4-N and NO2-N. As a result, aerobic nitrification of human urine was performed, with above 93.6% nitrification percentage finally achieved with total-N (TN) load of 46.52 mg/(L·d), in which Arthrospira platensis was successfully grown. The main compositions of the obtained biomass are close to those in Zarrouk medium. Thus, it is possible to culture Arthrospira platensis in nitrified human urine for food production within bioregenerative life support systems (BLSSs).

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Ahn, Y.H., 2006. Sustainable nitrogen elimination biotechnologies: A review. Process Biochemistry, 41(8):1709-1721.

[2] Alenka, P., Ivan, M., France, M., Eldor, A.P., James, M.T., 1998. Effects of pH and oxygen and ammonium concentrations on the community structure of nitrifying bacteria from wastewater. Applied and Environmental Microbiology, 64(10):3584-3590.

[3] APHA, AWWA, WEF, 1995. Standard Methods for Water and Wastewater Examination. Washington DC.

[4] Belay, A., 2002. The potential application of Spirulina (Arthrospira) as a nutritional and therapeutic supplement in health management. The Journal of the American Nutraceutical Association, 5(2):27-48.

[5] Blüm, V., Andriske, M., Ludwig, C.H., Paaflen, U., Voeste, D., 2003. The “C.E.B.A.S. mini-module”: A self-sustaining closed aquatic ecosystem for spaceflight experimentation. Advances in Space Research, 31(1):201-210.

[6] Carvajal, N., Fernandez, M., Rodrıguez, J.P., Donoso, M., 1980. Urease of Arthrospira maxima. Phytochemistry, 21(12):2821-2823.

[7] Chamorro, G., 1980. Toxicological Research on the Alga Arthrospira (in French). UNIDO, UF/MEX/78/048.

[8] Colliver, B.B., Stephenson, T., 2000. Production of nitrogen oxide and dinitrogen oxide by autotrophic nitrifiers. Biotechnology Advances, 18(3):219-232.

[9] Costa, J.A.V., Colla, L.M., Filho, P.F.D., 2004. Improving Spirulina platensis biomass yield using a fed-batch process. Bioresource Technology, 92(3):237-241.

[10] Danesi, E.D.G., Rangel-Yagui, C.O., de Carvalho, J.C.M., Sato, S., 2002. An investigation of effect of replacing nitrate by urea in the growth and production of chlorophyll by Arthrospira platensis. Biomass and Bioenergy, 23:261-269.

[11] Feng, D.L., Wu, Z.C., 2006. Culture of Arthrospira platensis in human urine for biomass production and O2 evolution. Journal of Zhejiang University SCIENCE B, 7(1):34-37.

[12] Gòdia, F., Albiol, J., Montesinos, J.L., Pérez, J., Creus, N., Cabello, F., Mengual, X., Montras, A., Lasseur, C., 2002. MELISSA: A loop of interconnected bioreactors to develop life support in space. Journal of Biotechnology, 99(3):319-330.

[13] Gordon, R. (Ed.), 1982. Essentials of Human Physiology (2nd Ed.). Year Book Medical Publishers, Chicago.

[14] Kowalchuk, G.A, Stephen, J.R., 2001. Ammonia-oxidizing bacteria: A model for molecular microbial ecology. Annual Review of Microbiology, 55(1):485-529.

[15] Larsen, T.A., Gujer, W., 1996. Separate management of anthropogenic nutrient solutions (human urine). Water Science and Technology, 34(3-4):87-94.

[16] Mota, C., Ridenoure, J., Cheng, J.Y., de los Reyes III, F.L., 2005. High levels of nitrifying bacteria in intermittently aerated reactors treating high ammonia wastewater. FEMS Microbiology Ecology, 54(3):391-400.

[17] Ogbonna, J.C., Yadam, H., Tanaka, H., 1995. Light supply coefficient: A new engineering parameter for photobioreactor design. Journal of Fermentation and Bioengineering, 80(4):369-376.

[18] Rangel-Yagui, C.O., Danesi, E.D.G., de Carvalho, J.C.M., Sato, S., 2004. Chlorophyll production from Spirulina platensis: cultivation with urea addition by fed-batch process. Bioresource Technology, 92(2):133-141.

[19] Richmond, A., 1992. Mass Culture of Cyanobacteria. In: Mann, N.H., Carr, N.G. (Eds.), Photosynthetic Prokaryotes, 2nd Ed. Plenum Press, New York and London, p.181-210.

[20] Rodushkin, I., Ödman, F., 2001. Application of inductively coupled mass spectrometry for elemental plasma sector field analysis of urine. Journal of Trace Elements in Medicine and Biology, 14(4):241-247.

[21] Ruiz, G., Jeison, D., Chamy, R., 2003. Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration. Water Research, 37(6):1371-1377.

[22] Salisbury, F.B., Gitelson, J.I., 1997. BIOS-3: Siberian experiments in bioregenerative life support. Bioscience, 47(9):575-585.

[23] Soletto, D., Binaghi, L., Lodi, A., de Carvalho, J.C.M., Converti, A., 2005. Batch and fed-batch cultivations of Spirulina platensis using ammonium sulphate and urea as nitrogen sources. Aquaculture, 243(1-4):217-224.

[24] Stein, L.Y., Arp, D.J., 1998. Loss of ammonia monooxygenase activity in Nitrosomonas europaea upon exposure to nitrite. Applied and Environmental Microbiology, 64(10):4098-4102.

[25] Sullivan, D.M., Carpenter, D.E., 1993. Methods of Analysis for Nutrition Labeling. AOAC International, Arlington, VA, p.85-104.

[26] Tadros, M.G., Smith, W., Mbuthia, P., Joseph, B., 1991. Manipulating Cyanobacteria: Arthrospira for Potential CELSS Diet. In: Alabama, A., Univ, M. (Eds.), NASA-HBCU Space Science and Engineering Research Forum Proceedings. USA, p.40-44.

[27] Udert, K.M., Fux, C., Münster, M., Larsen, T.A., Siegrist, H., Gujer, W., 2003. Nitrification and autotrophic denitrification of source-separated urine. Water Science and Technology, 48:119-130.

[28] Wheeler, R.M., Sager, J.C., Knott, W.M., Mackowiak, C.L., Stutte, G.W., Yorio, N.C., Ruffe, L.M., Peterson, B.V., Goins, G.D., Hinkle, C.R., Berry, W.L., 2003. Crop Production for Advanced Life Support Systems—Observations from the Kennedy Space Center Breadboard Project. NASA/TM–2003-211184.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE