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Received: 2007-05-08

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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.9 P.661~665

http://doi.org/10.1631/jzus.2007.B0661


Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea


Author(s):  LU Jian-ping, DUAN Zhi-bing, LIU Tong-bao, LIN Fu-cheng

Affiliation(s):  Department of Biology, School of Life Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   jplu@zju.edu.cn, fuchenglin@zju.edu.cn

Key Words:  Magnaporthe grisea, Promoter, NAR, Clone, Hypha


LU Jian-ping, DUAN Zhi-bing, LIU Tong-bao, LIN Fu-cheng. Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea[J]. Journal of Zhejiang University Science B, 2007, 8(9): 661~665.

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author="LU Jian-ping, DUAN Zhi-bing, LIU Tong-bao, LIN Fu-cheng",
journal="Journal of Zhejiang University Science B",
volume="8",
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pages="661~665",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0661"
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%T Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea
%A LU Jian-ping
%A DUAN Zhi-bing
%A LIU Tong-bao
%A LIN Fu-cheng
%J Journal of Zhejiang University SCIENCE B
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%N 9
%P 661~665
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%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0661

TY - JOUR
T1 - Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea
A1 - LU Jian-ping
A1 - DUAN Zhi-bing
A1 - LIU Tong-bao
A1 - LIN Fu-cheng
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 9
SP - 661
EP - 665
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2007.B0661


Abstract: 
The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the “TATA” box, the “CAAT” box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences indicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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