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On-line Access: 2024-08-27

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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.9 P.661-665

http://doi.org/10.1631/jzus.2007.B0661


Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea


Author(s):  LU Jian-ping, DUAN Zhi-bing, LIU Tong-bao, LIN Fu-cheng

Affiliation(s):  Department of Biology, School of Life Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   jplu@zju.edu.cn, fuchenglin@zju.edu.cn

Key Words:  Magnaporthe grisea, Promoter, NAR, Clone, Hypha


LU Jian-ping, DUAN Zhi-bing, LIU Tong-bao, LIN Fu-cheng. Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea[J]. Journal of Zhejiang University Science B, 2007, 8(9): 661-665.

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author="LU Jian-ping, DUAN Zhi-bing, LIU Tong-bao, LIN Fu-cheng",
journal="Journal of Zhejiang University Science B",
volume="8",
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pages="661-665",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0661"
}

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%T Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea
%A LU Jian-ping
%A DUAN Zhi-bing
%A LIU Tong-bao
%A LIN Fu-cheng
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 9
%P 661-665
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0661

TY - JOUR
T1 - Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea
A1 - LU Jian-ping
A1 - DUAN Zhi-bing
A1 - LIU Tong-bao
A1 - LIN Fu-cheng
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 9
SP - 661
EP - 665
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0661


Abstract: 
The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the “TATA” box, the “CAAT” box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences indicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25(17):3389-3402.

[2] Bruno, K.S., Tenjo, F., Li, L., Hamer, J.E., Xu, J.R., 2004. Cellular localization and role of kinase activity of PMK1 in Magnaporthe grisea. Eukaryotic Cell, 3(6):1525-1532.

[3] Carroll, A.M., Sweigard, J.A., Valent, B., 1994. Improved vectors for selecting resistance to hygromycin. Fungal Genetics Newsletter, 41:22.

[4] Dean, R.A., Talbot, N.J., Ebbole, D.J., Farman, M.L., Mitchell, T.K., Orbach, M.J., Thon, M., Kulkarni, R., Xu, J.R., Pan, H., et al., 2005. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature, 434(7036):980-986.

[5] Foster, A.J., Jenkinson, J.M., Talbot, N.J., 2003. Trehalose synthesis and metabolism are required at different stages of plant infection by Magnaporthe grisea. EMBO Journal, 22(2):225-235.

[6] Fudal, I., Collemare, J., Böhnert, H.U., Melayah, D., Lebrun, M., 2007. Expression of Magnaporthe grisea avirulence gene ACE1 is connected to the initiation of appressorium-mediated penetration. Eukaryotic Cell, 6(3):546-554.

[7] Hua, S.S.T., Baker, J.L., Flores-Espiritu, M., 1999. Interactions of saprophytic yeasts with a nor mutant of Aspergillus flavus. Applied and Environmental Microbiology, 65(6):2738-2740.

[8] Liu, X.H., Lu, J.P., Zhang, L., Dong, B., Min, H., Lin, F.C., 2007. Involvement of a Magnaporthe grisea serine/threonine kinase gene, MgATG1, in appressorium turgor and pathogenesis. Eukaryotic Cell, 6(6):997-1005.

[9] Lu, J.P., Liu, T.B., Yu, X.Y., Lin, F.C., 2005. Representative appressorium stage cDNA library of Magnaporthe grisea. Journal of Zhejiang University SCIENCE B, 6(12):132-136.

[10] Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.

[11] Soanes, D.M., Talbot, N.J., 2005. A bioinformatics tool for analysis of EST abundance during infection-related development by Magnaporthe grisea. Molecular Plant Pathology, 6(5):503-512.

[12] Talbot, N.J., Ebbole, D.J., Hamer, J.E., 1993. Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell, 5(11):1575-1590.

[13] Tournu, H., Tripathi, G., Bertram, G., Macaskill, S., Mavor, A., Walker, L., Odds, F.C., Gow, N.A.R., Brown, A.J.P., 2005. Global role of the protein kinase Gcn2 in the human pathogen Candida albicans. Eukaryotic Cell, 4(10):1687-1696.

[14] Xue, C., Park, G., Choi, W., Zheng, L., Dean, R.A., Xu, J.R., 2002. Two novel fungal virulence genes specifically expressed in appressoria of the rice blast fungus. Plant Cell, 14(9):2107-2119.

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