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On-line Access: 2016-09-08

Received: 2015-10-29

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Crosschecked: 2016-08-18

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Yan-jie Wei


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Journal of Zhejiang University SCIENCE A 2016 Vol.17 No.9 P.745-757


Membrane fouling behavior and microbial community succession in a submerged membrane bioreactor treating harbor oily wastewater

Author(s):  Yan-jie Wei, Guo-yi Li

Affiliation(s):  Key Laboratory of Environmental Protection in Water Transport Engineering, Tianjin Research Institute for Water Transport Engineering, Tianjin 300456, China

Corresponding email(s):   wei_yj@126.com

Key Words:  Membrane bioreactor (MBR) fouling, Extracellular polymeric substances (EPS), Harbor oily wastewater, Microbial community

Yan-jie Wei, Guo-yi Li. Membrane fouling behavior and microbial community succession in a submerged membrane bioreactor treating harbor oily wastewater[J]. Journal of Zhejiang University Science A, 2016, 17(9): 745-757.

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author="Yan-jie Wei, Guo-yi Li",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Membrane fouling behavior and microbial community succession in a submerged membrane bioreactor treating harbor oily wastewater
%A Yan-jie Wei
%A Guo-yi Li
%J Journal of Zhejiang University SCIENCE A
%V 17
%N 9
%P 745-757
%@ 1673-565X
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A1500289

T1 - Membrane fouling behavior and microbial community succession in a submerged membrane bioreactor treating harbor oily wastewater
A1 - Yan-jie Wei
A1 - Guo-yi Li
J0 - Journal of Zhejiang University Science A
VL - 17
IS - 9
SP - 745
EP - 757
%@ 1673-565X
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.A1500289

A membrane bioreactor (MBR) was established for treating harbor oily wastewater. It showed good removal performance for chemical oxygen demand (COD), oil content, suspended solids (SS), and other pollutants. However, serious membrane fouling occurred. It was recognized that the extracellular polymeric substances (EPS) accumulated on the membrane surface, especially the proteins, were of great importance for the transmembrane pressure (TMP) increment and membrane fouling. The MBR was optimized via improving aeration rate and reducing the ratio of Ar/Ad (Ar and Ad are the cross-sectional areas of the riser and the downcomer of the MBR). The increasing rate of TMP was slowed, indicating that the optimization strategy could effectively mitigate membrane fouling. microbial community evolution was monitored and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), cloning, and sequencing of 16S ribosomal ribonucleic acid (rRNA) fragments. Results revealed that low community shift occurred during the whole operational period. Geobacter sp. and Rhodocyclales sp., which have also been identified by other studies in a petroleum refinery wastewater MBR or an infiltration basin receiving highway runoff, dominated in the MBR system throughout. Comamonas sp. was thought to accommodate the lower aeration rate in this study, while Rhodocyclales sp. preferred the higher aeration rate. In addition, during the operational time under high organic loading rate, a few species were present in abundance, and may have been responsible for the good removal performance at this time.

The authors described the establishment of a membrane bioreactor (MBR) for the treatment of harbor oily wastewater, and investigated with different approaches the causes of membrane fouling, a severe limitation to the development of MBR technology. Particularly, the Authors explored the relationship among the numerous factors that can lead to membrane fouling, with the aim to identify the key factors in this complex mechanism. In particular, it was deeply explored the role of the extracellular polymeric substances, and examined their content and composition also in an optimized MBR system. In addition, an interesting characterization of the microbial population during the different operational stages was performed. The study is of interest since it has undoubtedly a great value from an application point of view. The study is well conducted and convincing, particularly thanks to the use of different complementary approaches that made it possible to disclose the crucial factors that determine the membraner fouling.


创新点:1. 分析出膜表面累积的胞外蛋白是膜污染严重和过膜压力增大的关键诱因;2. 基于Yusuf Chisti和Murray Moo Young提出的气提液体上升流速模型,结合胞外蛋白变化趋势,提出降低过膜压力上升速度和缓解膜污染进程的有效措施;3. 针对处理港口含油废水的生物系统进行种群结构研究及优势菌属的分离与鉴定。
方法:1. 借助环境扫描电子显微镜(SEM)、能量色散X射线光谱仪(EDX)和傅里叶红外光谱仪(FTIR)等分析手段表征膜污染物的主要成分;2. 基于Yusuf Chisti和Murray Moo Young提出的气提液体上升流速模型,提出缓解膜污染进程的措施,并验证所提措施的有效性(图3和9);3. 通过巢式PCR-DGGE技术对处理港口含油废水的膜生物反应器不同运行阶段的微生物群落结构进行研究,并采用克隆技术对优势微生物进行菌种鉴定(图10和表3)。
结论:1. 采用一体化厌氧/好氧-膜生物反应器来处理港口含油废水的优点是对有机污染物和油类等污染物的去除效率高,但在其运行过程中膜污染严重。2. 分析发现胞外聚合物是引起膜污染物的主要因素(图3~6);组分分析进一步表明膜表面累积的胞外蛋白是膜污染严重和过膜压力增大的关键诱因(图7~9)。3. 通过增大反应器曝气量,调整反应器结构参数可有效降低过膜压力上升速度,缓解膜污染进程。4. 反应器内细菌群落结构平稳。5. 克隆测序结果表明:膜生物反应器内丛毛单胞菌属在低曝气环境下繁殖能力强,且可能是引起膜污染的特征微生物;红环菌科细菌更适应高曝气条件,是降解含有油类污染物的特征细菌。


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