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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.4 P.306~312

http://doi.org/10.1631/jzus.B0710625


A preliminary study on the teratogenesis of dexamethasone and the preventive effect of vitamin B12 on murine embryonic palatal shelf fusion in vitro


Author(s):  Sheng-jun LU, Wei HE, Bing SHI, Tian MENG, Xiao-yu LI, Yu-rong LIU

Affiliation(s):  State Key Laboratory of Oral Disease, West China College of Stomatology, Sichuan University, Chengdu 610041, China; more

Corresponding email(s):   shibingcn@sina.com, tianmeng_scu@yahoo.com.cn

Key Words:  Dexamethasone, Vitamin B12, Organ culture, Cleft palate, Medial edge epithelial cells


Sheng-jun LU, Wei HE, Bing SHI, Tian MENG, Xiao-yu LI, Yu-rong LIU. A preliminary study on the teratogenesis of dexamethasone and the preventive effect of vitamin B12 on murine embryonic palatal shelf fusion in vitro[J]. Journal of Zhejiang University Science B, 2008, 9(4): 306~312.

@article{title="A preliminary study on the teratogenesis of dexamethasone and the preventive effect of vitamin B12 on murine embryonic palatal shelf fusion in vitro",
author="Sheng-jun LU, Wei HE, Bing SHI, Tian MENG, Xiao-yu LI, Yu-rong LIU",
journal="Journal of Zhejiang University Science B",
volume="9",
number="4",
pages="306~312",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0710625"
}

%0 Journal Article
%T A preliminary study on the teratogenesis of dexamethasone and the preventive effect of vitamin B12 on murine embryonic palatal shelf fusion in vitro
%A Sheng-jun LU
%A Wei HE
%A Bing SHI
%A Tian MENG
%A Xiao-yu LI
%A Yu-rong LIU
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 4
%P 306~312
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0710625

TY - JOUR
T1 - A preliminary study on the teratogenesis of dexamethasone and the preventive effect of vitamin B12 on murine embryonic palatal shelf fusion in vitro
A1 - Sheng-jun LU
A1 - Wei HE
A1 - Bing SHI
A1 - Tian MENG
A1 - Xiao-yu LI
A1 - Yu-rong LIU
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 4
SP - 306
EP - 312
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0710625


Abstract: 
Excessive dexamethasone (Dex) administrated into pregnant mice during critical periods of palatal development can produce a high incidence of cleft palate. Its mechanisms remain unknown. vitamin B12 has been shown to antagonize the teratogenic effects of Dex, which, however, remains controversial. In this study, we investigated the effects of Dex and vitamin B12 on murine embryonic palatal shelf fusion using organ culture of murine embryonic shelves. The explanted palatal shelves on embryonic day 14 (E14) were cultured for 24, 48, 72 or 96 h in different concentrations of Dex and/or vitamin B12. The palatal shelves were examined histologically for the morphological alterations on the medial edge epithelium (MEE) and fusion rates among different groups. It was found that the palatal shelves were not fused at 72 h or less of culture in Dex group, while they were completely fused in the control and vitamin B12-treated groups at 72 and 96 h, respectively. The MEE still existed and proliferated. In Dex+vitamin B12 group the palatal shelves were fused at each time point in a similar rate to controls. These results may suggest that Dex causes teratogenesis of murine embryonic palatal shelves and vitamin B12 prevents the teratogenic effect of Dex on palatogenesis on murine embryos in vitro.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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