Full Text:   <1849>

CLC number: S85

On-line Access: 

Received: 2009-03-12

Revision Accepted: 2009-06-10

Crosschecked: 2009-09-04

Cited: 17

Clicked: 3594

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.10 P.753~760


Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China

Author(s):  Jian-ping LI, Hai-jian ZHOU, Lin YUAN, Ting HE, Song-hua HU

Affiliation(s):  Department of Veterinary Medicine, College of Animal Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   Songhua@zju.edu.cn

Key Words:  Staphylococcus aureus, Bovine mastitis, Antimicrobial resistance, Genetic diversity, Pulsed-field gel electrophoresis

Jian-ping LI, Hai-jian ZHOU, Lin YUAN, Ting HE, Song-hua HU. Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China[J]. Journal of Zhejiang University Science B, 2009, 10(10): 753~760.

@article{title="Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China",
author="Jian-ping LI, Hai-jian ZHOU, Lin YUAN, Ting HE, Song-hua HU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China
%A Jian-ping LI
%A Hai-jian ZHOU
%A Ting HE
%A Song-hua HU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 10
%P 753~760
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920072

T1 - Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China
A1 - Jian-ping LI
A1 - Hai-jian ZHOU
A1 - Lin YUAN
A1 - Ting HE
A1 - Song-hua HU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 10
SP - 753
EP - 760
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920072

This study was conducted to determine genetic diversity and antimicrobial susceptibility profiles of Staphylococcus aureus recovered from bovine mastitis in Zhejiang Province, China. Out of 3178 quarter milk samples from 846 lactating cows, among which 459 cows (54.3%) were found HMT positive, 890 quarters (28%) were found having subclinical mastitis. From 75 representative S. aureus isolates, 16 distinct types were identified by pulsed-field gel electrophoresis (PFGE). Four major PFGE types (A, B, C, and D) accounted for 82.7% of all isolates, and type A (41.3%) was observed in multiple herds across the studied areas. Each region was found to have a predominant type: Hangzhou type A (64.1%), Ningbo type C (34.5%) and type B (23.1%), Jinhua type D (53.3%), and Taizhou type C (62.5%). Results of antimicrobial susceptibility tests showed that 90.7% of the isolates were resistant to at least one antimicrobial. Resistance to penicillin and ampicillin (77.3%), tetracycline (60.0%), or erythromycin (48.0%) was observed. The bacteria resistant to multiple antibiotics such as penicillin, ampicillin, tetracycline, and erythromycin were commonly found. The information obtained from this study is useful for designing specific control programs for bovine S. aureus mastitis in this region.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Anderson, K.L., Lyman, R.L., Bodeis-Jones, S.M., White, D.G., 2006. Genetic diversity and antimicrobial susceptibility profiles among mastitis-causing Staphylococcus aureus isolated from bovine milk samples. Am. J. Vet. Res., 67(7): 1185-1191.

[2] Archer, G.L., 1998. Staphylococcus aureus: a well-armed pathogen. Clin. Infect. Dis., 26(5):1179-1181.

[3] Bradley, A.J., 2002. Bovine mastitis: an evolving disease. Vet. J., 164(2):116-128.

[4] de Oliveira, A.P., Watts, J.L., Salmon, S.A., Aarestrup, F.M., 2000. Antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Europe and the United States. J. Dairy Sci., 83(4):855-862.

[5] Ferguson, J.D., Azzaro, G., Gambina, M., Licitra, G., 2007. Prevalence of mastitis pathogens in Ragusa, Sicily, from 2000 to 2006. J. Dairy Sci., 90(12):5798-5813.

[6] Fox, L.K., Gershman, M., Hancock, D.D., Hutton, C., 1991. Fomites and reservoirs of Staphylococcus aureus intramammary infections: the effect of milking time hygiene. Cornell Vet., 81(2):183-193.

[7] Fueyo, J.M., Mendoza, M.C., Rodicio, M.R., Muñiz, J., Alvarez, M.A., Martín, M.C., 2005. Cytotoxin and pyrogenic toxin superantigen gene profiles of Staphylococcus aureus associated with subclinical mastitis in dairy cows and relationships with macrorestriction genomic profiles. J. Clin. Microbiol., 43(3):1278-1284.

[8] Gentilini, E., Denamiel, G., Llarente, P., Godaly, S., Rebuelto, M., DeGregorio, O., 2000. Antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Argentina. J. Dairy Sci., 83(6):1224-1227.

[9] Haveri, M., Roslöf, A., Rantala, L., Pyörälä, S., 2007. Virulence genes of bovine Staphylococcus aureus from persistent and nonpersistent intramammary infections with different clinical characteristics. J. Appl. Microbiol., 103(4):993-1000.

[10] Hu, S.H., Zhu, P.M., Jiang, C.S., 1990. The relationship between reaction grades of Hangzhou mastitis test (HMT) agent and milk somatic cell counts. Chin. J. Vet. Med., 16(10):8-9 (in Chinese).

[11] Hu, S.H., Du, A.F., Cai, W.M., 1998. Bacteriological analysis of bovine clinical and subclinical mastitis. Chin. J. Anim. Poul. Infect. Dis., 20(4):199-201 (in Chinese).

[12] Joo, Y.S., Fox, L.K., Davis, W.C., Bohach, G.A., Park, Y.H., 2001. Staphylococcus aureus associated with mammary glands of cows: genotyping to distinguish different strains among herds. Vet. Microbiol., 80(2):131-138.

[13] Kaneene, J.B., Miller, R., 1992. Description and evaluation of the influence of veterinary presence on the use of antibiotics and sulfonamides in dairy herds. J. Am. Vet. Med. Assoc., 201(1):68-76.

[14] Liu, C., Wang, J.R., Zhang, C.D., Wu, B., Han, Y.P., Zhao, B., Yu, Y.J., Wang, J.H., Liu, Y.B., Cheng, G.B., Feng, Z., Tian, D., Chen, H.C., Guo, A.Z., 2007. Isolation, identification and analysis of drug resistance of mastitis-causing pathogens from Hubei. China Dairy Cattle, 7:35-38 (in Chinese).

[15] Lowy, F.D., 2003. Antimicrobial resistance: the example of Staphylococcus aureus. J. Clin. Invest., 111(9):1265-1273.

[16] McDougal, L.K., Steward, C.D., Killgore, G.E., Chaitram, J.M., McAllister, S.K., Tenover, F.C., 2003. Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the United States: establishing a national database. J. Clin. Microbiol., 41(11):5113-5120.

[17] Miles, H., Lesser, W., Sears, P., 1992. The economic implications of bioengineered mastitis control. J. Dairy Sci., 75(2):596-605.

[18] Moroni, P., Pisoni, G., Antonini, M., Villa, R., Boettcher, P., Carli, S., 2006. Antimicrobial drug susceptibility of Staphylococcus aureus from subclinical bovine mastitis in Italy. J. Dairy Sci., 89(8):2973-2976.

[19] NCCLS (National Committee for Clinical Laboratory Standards), 2002. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Approved Standard. NCCLS Document M31-A2, Wayne, PA.

[20] National Mastitis Council, 1999. Laboratory Handbook on Bovine Mastitis. National Mastitis Council, Inc., p.71-76.

[21] Oliver, S.P., Gillespie, B.E., Headrick, S.J., Moorehead, A., Lunn, P., Dowlen, H.H., Johnson, D.L., Lamar, K.C., Chester, S.T., Moseley, W.M., 2004. Efficacy of extended ceftiofur intramammary therapy for treatment of subclinical mastitis in lactating dairy cows. J. Dairy Sci., 87(8):2393-2400.

[22] Østeras, O., Sølverød, L., Reksen, O., 2006. Milk culture results in a large Norwegian survey: effects of season, parity, days in milk, resistance, and clustering. J. Dairy Sci., 89(3):1010-1023.

[23] Owens, W.E., Watts, J.L., Boddie, R.L., Nickerson, S.C., 1988. Antibiotic treatment of mastitis: comparison of intramammary and intramammary plus intramuscular therapies. J. Dairy Sci., 71(11):3143-3147.

[24] Pan, H., Liu, C.C., Zhang, L.H., Zhang, Z.C., Yu, J., Yuan, Y.L., Li, H.S., Hou, Y.Z., Yang, Y.Y., Li, X.P., 1996. Etiology and pathogenesis survey of bovine mastitis in some regions of China. Chin. J. Vet. Sci. Technol., 26(2):16-17 (in Chinese).

[25] Peacock, S.J., de Silva, G.D., Justice, A., Cowland, A., Moore, C.E., Winearls, C.G., Day, N.P., 2002. Comparison of multilocus sequence typing and pulsed-field gel electrophoresis as tools for typing Staphylococcus aureus isolates in a microepidemiological Setting. J. Clin. Microbiol., 40(10):3764-3770.

[26] Pitkala, A., Haveri, M., Pyorala, S., Myllys, V., Honkanen-Buzalski, T., 2004. Bovine mastitis in Finland 2001: Prevalence, distribution of bacteria, and antimicrobial resistance. J. Dairy Sci., 87(8):2433-2441.

[27] Rabello, R.F., Souza, C.R.V.M., Duarte, R.S., Lopes, R.M.M., Teixeira, L.M., Castro, A.C.D., 2005. Characterization of Staphylococcus aureus isolates recovered from bovine mastitis in Rio de Janeiro, Brazil. J. Dairy Sci., 88(9): 3211-3219.

[28] Sabour, P.M., Gill, J.J., Lepp, D., Pacan, J.C., Ahmed, R., Dingwell, R., Leslie, K., 2004. Molecular typing and distribution of Staphylococcus aureus isolates in eastern Canadian dairy herds. J. Clin. Microbiol., 42(8): 3449-3455.

[29] Sandgren, C.H., Waller, K.P., Emanuelson, U., 2008. Therapeutic effects of systemic or intramammary antimicrobial treatment of bovine subclinical mastitis during lactation. Vet. J., 175(1):108-117.

[30] Shitandil, A., Sternesjö, Å., 2004. Prevalence of multidrug resistant Staphylococcus aureus in milk from large- and small-scale producers in Kenya. J. Dairy Sci., 87(12): 4145-4149.

[31] Smith, E.M., Green, L.E., Medley, G.F., Bird, H.E., Fox, L.K., Schukken, Y.H., Kruze, J.V., Bradley, A.J., Zadoks, R.N., Dowson, C.G., 2005. Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates. J. Clin. Microbiol., 43(9):4737-4743.

[32] Sol, J., Sampimon, O.C., Barkema, H.W., Schukken, Y.H., 2000. Factors associated with cure after therapy of clinical mastitis caused by Staphylococcus aureus. J. Dairy Sci., 83(2):278-284.

[33] Tenover, F.C., Arbeit, R.D., Goering, R.V., Mickelsen, P.A., Murray, B.E., Persing, D.H., Swaminathan, B., 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol., 33(9): 2233-2239.

[34] Waage, S., Bjorland, J., Caugant, D.A., Oppegaard, H., Tollersrud, T., Mrk, T., Aarestrup, F.M., 2002. Spread of Staphylococcus aureus resistant to penicillin and tetracycline within and between dairy herds. Epidemiol. Infect., 129(1):193-202.

[35] Wang, B., Liu, M.C., Sheng, J.Z., Shen, J.Z., 2007. Resistance survey of Staphylococcus aureus isolated from bovine mastitis in Hohhot. Chin. J. Vet. Med., 43(3):30-32 (in Chinese).

[36] Weller, T.M., 2000. Methicillin-resistant Staphylococcus aureus typing methods: which should be the international standard? J. Hosp. Infect., 44(3):160-172.

[37] Wilson, D.J., Gonzalez, R.N., Das, H.H., 1997. Bovine mastitis pathogens in New York and Pennsylvania: prevalence and effects on somatic cell count and milk production. J. Dairy Sci., 80(10):2592-2598.

[38] Zhang, X.J., Xu, S.L., Wu, Y., Wu, C.J., 2005. Prevalence of bovine subclinical mastitis in Jinhua. Chin. J. Anim. Sci., 41(12):37-38 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE