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Received: 2009-07-14

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Crosschecked: 2009-11-17

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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.12 P.928~932

http://doi.org/10.1631/jzus.B0920205


Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4+ CD25+ Treg cells


Author(s):  Hang YAN, Chen-guang DING, Pu-xun TIAN, Guan-qun GE, Zhan-kui JIN, Li-ning JIA, Xiao-ming DING, Xiao-ming PAN, Wu-jun XUE

Affiliation(s):  Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, Medicine College, Xi'an Jiaotong University, Xi'an 710061, China

Corresponding email(s):   yuantian@mail.xjtu.edu.cn

Key Words:  CD4+ CD25+ Treg cells, Flow cytometry sorting, Magnetic cell sorting


Hang YAN, Chen-guang DING, Pu-xun TIAN, Guan-qun GE, Zhan-kui JIN, Li-ning JIA, Xiao-ming DING, Xiao-ming PAN, Wu-jun XUE. Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4+ CD25+ Treg cells[J]. Journal of Zhejiang University Science B, 2009, 10(12): 928~932.

@article{title="Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4+ CD25+ Treg cells",
author="Hang YAN, Chen-guang DING, Pu-xun TIAN, Guan-qun GE, Zhan-kui JIN, Li-ning JIA, Xiao-ming DING, Xiao-ming PAN, Wu-jun XUE",
journal="Journal of Zhejiang University Science B",
volume="10",
number="12",
pages="928~932",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0920205"
}

%0 Journal Article
%T Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4+ CD25+ Treg cells
%A Hang YAN
%A Chen-guang DING
%A Pu-xun TIAN
%A Guan-qun GE
%A Zhan-kui JIN
%A Li-ning JIA
%A Xiao-ming DING
%A Xiao-ming PAN
%A Wu-jun XUE
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 12
%P 928~932
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920205

TY - JOUR
T1 - Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4+ CD25+ Treg cells
A1 - Hang YAN
A1 - Chen-guang DING
A1 - Pu-xun TIAN
A1 - Guan-qun GE
A1 - Zhan-kui JIN
A1 - Li-ning JIA
A1 - Xiao-ming DING
A1 - Xiao-ming PAN
A1 - Wu-jun XUE
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 12
SP - 928
EP - 932
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920205


Abstract: 
Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4+ CD25+ regulatory T (Treg) cells, in order to inform further studies in Treg cell function. Methods: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4+ CD25+ Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4+ CD25+ Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4+ CD25+ Treg cells in the in vitro proliferation experiments. Results: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4+ CD25+ Treg cells, however, obtained with both methods have similar immunosuppressive capacities. Conclusion: The result suggests that both methods can be used in isolating CD4+ CD25+ Treg cells, and one can select the best method according to specific needs and availability of the methodologies.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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