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CLC number: Q73

On-line Access: 2010-12-09

Received: 2010-04-06

Revision Accepted: 2010-11-15

Crosschecked: 2010-11-18

Cited: 14

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.12 P.923-930


Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes

Author(s):  Xiao-feng Lu, Guo-qing He, Hai-ning Yu, Qi Ma, Sheng-rong Shen, Undurti N. Das

Affiliation(s):  Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310032, China, UND Life Sciences, Shaker Heights, OH 44120, USA, College of Medicine, Bharati Vidyapeeth University, Pune 411030, India

Corresponding email(s):   shrshen@zju.edu.cn

Key Words:  Anti-cancer, Fatty acids composition, Linoleic acid, In vitro

Xiao-feng Lu, Guo-qing He, Hai-ning Yu, Qi Ma, Sheng-rong Shen, Undurti N. Das. Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes[J]. Journal of Zhejiang University Science B, 2010, 11(12): 923-930.

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author="Xiao-feng Lu, Guo-qing He, Hai-ning Yu, Qi Ma, Sheng-rong Shen, Undurti N. Das",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes
%A Xiao-feng Lu
%A Guo-qing He
%A Hai-ning Yu
%A Qi Ma
%A Sheng-rong Shen
%A Undurti N. Das
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 12
%P 923-930
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000125

T1 - Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes
A1 - Xiao-feng Lu
A1 - Guo-qing He
A1 - Hai-ning Yu
A1 - Qi Ma
A1 - Sheng-rong Shen
A1 - Undurti N. Das
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 12
SP - 923
EP - 930
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000125

Polyunsaturated fatty acids (PUFAs) possess anti-cancer action both in vitro and in vivo. In the present study, we detected cell viability with methyl thiazolyl tetrazolium (MTT) assay and cell membrane permeability with propidium iodide (PI) fluorescence dyeing, and calculated cell membrane fluidity change as fluorescence anisotropy. Fatty acid content in cells was measured by gas chromatography/mass spectroscopy (GC/MS), and the relationship between fatty acid composition and cell viability was studied. We observed that n-6 PUFA linoleic acid (LA) inhibited tumor cell growth at high concentrations (≥300 µmol/L), while low concentrations (100–200 µmol/L) seemed to promote cell proliferation. Analyses of cell membrane permeability, cell membrane fluidity, and cell fatty acid composition suggested that the anti-cancer action of LA could be related to changes in the ratio of n-6 to n-3 PUFAs. We observed that pre-incubation of cancer cells with 100 µmol/L LA for 24 h enhanced cell sensitivity to the cytotoxic action of LA, whereas undifferentiated cell line LoVo seemed to have a distinct path in LA-induced death. These results showed that one of the mechanisms by which supplementation of LA induces cancer cell death could be altering the ratio of n-6/n-3 PUFAs, and this may be related to cell differentiation status.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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