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CLC number: X173

On-line Access: 2014-09-18

Received: 2014-04-18

Revision Accepted: 2014-09-17

Crosschecked: 2015-01-07

Cited: 11

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Citations:  Bibtex RefMan EndNote GB/T7714


Dan Liu


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.2 P.123-130


Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation

Author(s):  Dan LIU, Song LI, Ejazul ISLAM, Jun-ren CHEN, Jia-sen WU, Zheng-qian YE, Dan-li PENG, Wen-bo YAN, Kou-ping LU

Affiliation(s):  Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, School of Environmental and Resource Sciences, Zhejiang A & F University, Lin’an 311300, China; more

Corresponding email(s):   liudan7812@aliyun.com

Key Words:  Moso bamboo, Pb, Phytoremediation, Scanning electron microscopy, Transmission electron microscopy

Dan LIU, Song LI, Ejazul ISLAM, Jun-ren CHEN, Jia-sen WU, Zheng-qian YE, Dan-li PENG, Wen-bo YAN, Kou-ping LU. Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation[J]. Journal of Zhejiang University Science B, 2015, 16(2): 123-130.

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author="Dan LIU, Song LI, Ejazul ISLAM, Jun-ren CHEN, Jia-sen WU, Zheng-qian YE, Dan-li PENG, Wen-bo YAN, Kou-ping LU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation
%A Dan LIU
%A Song LI
%A Ejazul ISLAM
%A Jun-ren CHEN
%A Jia-sen WU
%A Zheng-qian YE
%A Dan-li PENG
%A Wen-bo YAN
%A Kou-ping LU
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 2
%P 123-130
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400107

T1 - Lead accumulation and tolerance of Moso bamboo (Phyllostachys pubescens) seedlings: applications of phytoremediation
A1 - Dan LIU
A1 - Song LI
A1 - Ejazul ISLAM
A1 - Jun-ren CHEN
A1 - Jia-sen WU
A1 - Zheng-qian YE
A1 - Dan-li PENG
A1 - Wen-bo YAN
A1 - Kou-ping LU
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 2
SP - 123
EP - 130
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400107

A hydroponics experiment was aimed at identifying the lead (pb) tolerance and phytoremediation potential of moso bamboo (Phyllostachys pubescens) seedlings grown under different pb treatments. Experimental results indicated that at the highest pb concentration (400 μmol/L), the growth of bamboo seedlings was inhibited and pb concentrations in leaves, stems, and roots reached the maximum of 148.8, 482.2, and 4282.8 mg/kg, respectively. scanning electron microscopy revealed that the excessive pb caused decreased stomatal opening, formation of abundant inclusions in roots, and just a few inclusions in stems. The ultrastructural analysis using transmission electron microscopy revealed that the addition of excessive pb caused abnormally shaped chloroplasts, disappearance of endoplasmic reticulum, shrinkage of nucleus and nucleolus, and loss of thylakoid membranes. Although ultrastructural analysis revealed some internal damage, even the plants exposed to 400 µmol/L pb survived and no visual pb toxicity symptoms such as necrosis and chlorosis were observed in these plants. Even at the highest pb treatment, no significant difference was observed for the dry weight of stem compared with controls. It is suggested that use of moso bamboo as an experimental material provides a new perspective for remediation of heavy metal contaminated soil owing to its high metal tolerance and greater biomass.


方法:根系形态分析(图3)、植物重金属含量分析 (表1)、扫描电镜分析(图4)和透射电镜分析(图5)。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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