Full Text:   <1790>

CLC number: R604

On-line Access: 2013-01-08

Received: 2012-04-07

Revision Accepted: 2012-06-07

Crosschecked: 2012-12-12

Cited: 0

Clicked: 3950

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.1 P.68-75

http://doi.org/10.1631/jzus.B1200112


Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum


Author(s):  Xia-zhen Yu, Tao Ji, Xue-li Bai, Liang Liang, Lin-yan Wang, Wei Chen, Ting-bo Liang

Affiliation(s):  Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   liangtingbo@zju.edu.cn

Key Words:  Hepatic sinusoidal obstruction syndrome, Gynura segetum (Compositae), Sinusoidal endothelial cells, Matrix metalloproteinase-9


Xia-zhen Yu, Tao Ji, Xue-li Bai, Liang Liang, Lin-yan Wang, Wei Chen, Ting-bo Liang. Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum[J]. Journal of Zhejiang University Science B, 2013, 14(1): 68-75.

@article{title="Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum",
author="Xia-zhen Yu, Tao Ji, Xue-li Bai, Liang Liang, Lin-yan Wang, Wei Chen, Ting-bo Liang",
journal="Journal of Zhejiang University Science B",
volume="14",
number="1",
pages="68-75",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200112"
}

%0 Journal Article
%T Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum
%A Xia-zhen Yu
%A Tao Ji
%A Xue-li Bai
%A Liang Liang
%A Lin-yan Wang
%A Wei Chen
%A Ting-bo Liang
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 1
%P 68-75
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200112

TY - JOUR
T1 - Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum
A1 - Xia-zhen Yu
A1 - Tao Ji
A1 - Xue-li Bai
A1 - Liang Liang
A1 - Lin-yan Wang
A1 - Wei Chen
A1 - Ting-bo Liang
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 1
SP - 68
EP - 75
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200112


Abstract: 
Background and objective: hepatic sinusoidal obstruction syndrome (HSOS) is characterized by painful hepatomegaly, ascites, increased body weight, and jaundice. Gynura segetum (Compositae), a plant widely used in Chinese traditional medicine, often leads to the development of HSOS. However, the mechanism is unclear. The aim was to study the role of matrix metalloproteinase-9 (MMP-9) in the onset of HSOS induced by Gynura segetum. Methods: Twenty-five male Sprague-Dawley rats were randomly divided into two groups. Twenty were exposed to 600 mg/kg daily Gynura segetum extract solution for three weeks; five control rats were exposed to tap water alone. Liver sections were evaluated by light microscopy with a modified scoring system. Routine transmission electron microscopy (TEM) methods were used to evaluate the ultrastructual features of fixed liver tissue, and blood samples were collected to determine liver enzyme concentrations. MMP-9 expression was assessed by both immunohistochemical staining and enzyme-linked immunosorbent assay (ELISA) methods. Results: A stable and reproducible rat model of HSOS was achieved by long-term exposure to Gynura segetum extract. The treated rats presented clinical symptoms and the histopathological manifestation of HSOS, including abnormal liver enzyme concentrations (alanine aminotransferase (ALT): (84.8±13.62) vs. (167.0±72.63) U/L, P<0.05; aspartate aminotransferase (AST): (27.6±6.31) vs. (232.8±108.58) U/L, P<0.05). Hematoxylin and eosin (H&E) staining and TEM together revealed deposition of red blood cells, the damage and destruction of hepatic sinusoidal endothelial cells, collapse of hepatic sinusoids, hemorrhage of subendothelial cells, atrophy and destruction of hepatocytes, etc. Compared with controls, the expression of MMP-9 in the blood sample, the lung and liver tissues of HSOS rats was increased. Conclusions: MMP-9 may have an important role in early pathological changes of HSOS, and thus the onset of the disease.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Chen, M.Y., Cai, J.T., Du, Q., 2007. Hepatic veno-occlusive disease associated with the use of Gynura segetum. Europ. J. Intern. Med., 18(8):609.

[2]Chen, M.Y., Cai, J.T., Du, Q., Wang, L.J., Chen, J.M., Shao, L.M., 2008. Reliable experimental model of hepatic veno-occlusive disease caused by monocrotaline. Hepatobiliary Pancreat Dis. Int., 7(4):395-400.

[3]Chojkier, M., 2003. Hepatic sinusoidal-obstruction syndrome: toxicity of pyrrolizidine alkaloids. J. Hepatol., 39(3):437-446.

[4]DeLeve, L.D., 1994. Dacarbazine toxicity in murine liver cells: a model of hepatic endothelial injury and glutathione defense. J. Pharmacol. Exp. Ther., 268(3):1261-1270.

[5]DeLeve, L.D., 1996. Cellular target of cyclophosphamide toxicity in the murine liver: role of glutathione and site of metabolic activation. Hepatology, 24(4):830-837.

[6]DeLeve, L.D., Wang, X., Kuhlenkamp, J.F., Kaplowitz, N., 1996. Toxicity of azathioprine and monocrotaline in murine sinusoidal endothelial cells and hepatocytes: the role of glutathione and relevance to hepatic venoocclusive disease. Hepatology, 23(3):589-599.

[7]DeLeve, L.D., McCuskey, R.S., Wang, X., Hu, L., McCuskey, M.K., Epstein, R.B., Kanel, G.C., 1999. Characterization of a reproducible rat model of hepatic veno-occlusive disease. Hepatology, 29(6):1779-1791.

[8]DeLeve, L.D., Shulman, H.M., McDonald, G.B., 2002. Toxic injury to hepatic sinusoids: sinusoidal obstruction syndrome (veno-occlusive disease). Semin. Liver Dis., 22(1):27-42.

[9]DeLeve, L.D., Wang, X., Tsai, J., Kanel, G., Strasberg, S., Tokes, Z.A., 2003. Sinusoidal obstruction syndrome (veno-occlusive disease) in the rat is prevented by matrix metalloproteinase inhibition. Gastroenterology, 125(3):882-890.

[10]Fried, M.W., Duncan, A., Soroka, S., Connaghan, D.G., Farrand, A., Peter, J., Strauss, R.M., Boyer, T.D., McDonald, G.B., 2001. Serum hyaluronic acid in patients with veno-occlusive disease following bone marrow transplantation. Bone Marrow Transplant, 27(6):635-639.

[11]Hiller, O., Lichte, A., Oberpichler, A., Kocourek, A., Tschesche, H., 2000. Matrix metalloproteinases, collagenase-2, macrophage elastase, collagenase-3, and membrane type 1-matrix metalloproteinase impair clotting by degradation of fibrinogen and factor XII. J. Biol. Chem., 275(42):33008-33013.

[12]Kallianpur, A.R., Hall, L.D., Yadav, M., Byrne, D.W., Speroff, T., Dittus, R.S., Haines, J.L., Christman, B.W., Summar, M.L., 2005. The hemochromatosis C282Y allele: a risk factor for hepatic veno-occlusive disease after hematopoietic stem cell transplantation. Bone Marrow Transplant, 35(12):1155-1164.

[13]Kolettis, T., Vlahos, A.P., Louka, M., Hatzistergos, K.E., Baltogiannis, G.G., Agelaki, M.M., Mitsi, A., Malamou-Mitsi, V., 2007. Characterisation of a rat model of pulmonary arterial hypertension. Hellenic J. Cardiol., 48(4):206-210.

[14]Lin, G., Wang, J.Y., Li, N., Gao, H., Ji, Y., Zhang, F., Wang, H., Zhou, Y., Ye, Y., Xu, H.X., Zheng, J., 2011. Hepatic sinusoidal obstruction syndrome associated with consumption of Gynura segetum. J. Hepatol., 54(4):666-673.

[15]Maruyama, H., Watanabe, S., Kimura, T., Liang, J., Nagasawa, T., Onodera, M., Aonuma, K., Yamaguchi, I., 2007. Granulocyte colony-stimulating factor prevents progression of monocrotaline-induced pulmonary arterial hypertension in rats. Circ. J., 71(1):138-143.

[16]Nagase, H., Visse, R., Murphy, G., 2006. Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res., 69(3):562-573.

[17]Patterson, M.L., Atkinson, S.J., Knäuper, V., Murphy, G., 2001. Specific collagenolysis by gelatinase A, MMP-2, is determined by the hemopexin domain and not the fibronectin-like domain. FEBS Lett., 503(2-3):158-162.

[18]Petaja, J., Pitkanen, S., Vettenranta, K., Fasth, A., Heikinheimo, M., 2000. Serum tumor marker CA 125 is an early and sensitive indicator of veno-occlusive disease in children undergoing bone marrow transplantation. Clin. Cancer Res., 6:531-535.

[19]Richardson, P., Guinan, E., 1999. The pathology, diagnosis, and treatment of hepatic veno-occlusive disease: current status and novel approaches. Br. J. Haematol., 107(3):485-493.

[20]Rio, B., Bauduer, F., Arrago, J.P., Zittoun, R., 1993. N-terminal peptide of type III procollagen: a marker for the development of hepatic veno-occlusive disease after BMT and a basis for determining the timing of prophylactic heparin. Bone Marrow Transplant, 11(6):471-472.

[21]Stolow, M.A., Bauzon, D.D., Li, J., Sedgwick, T., Liang, V.C., Sang, Q.A., Shi, Y.B., 1996. Identification and characterization of a novel collagenase in Xenopus laevis: possible roles during frog development. Mol. Biol. Cell, 7(10):1471-1483.

[22]Tanikawa, S., Mori, S., Ohhashi, K., Akiyama, H., Sasaki, T., Kaku, H., Hiruma, K., Matsunaga, T., Morita, T., Sakamaki, H., 2000. Predictive markers for hepatic veno-occlusive disease after hematopoietic stem cell transplantation in adults: a prospective single center study. Bone Marrow Transplant, 26(8):881-886.

[23]Visse, R., Nagase, H., 2003. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ. Res., 92(8):827-839.

[24]Wadleigh, M., Ho, V., Momtaz, P., Richardson, P., 2003. Hepatic veno-occlusive disease: pathogenesis, diagnosis and treatment. Curr. Opin. Hematol., 10(6):451-462.

[25]Willmot, F.C., Robertson, G.W., 1920. Senecio disease, or cirrhosis of the liver due to Senecio poisoning. Lancet, 196(5069):848.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE