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On-line Access: 2015-04-03

Received: 2014-06-01

Revision Accepted: 2014-12-10

Crosschecked: 2015-03-17

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Fa-zhong Yang


Chun Xiao


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.4 P.264-274


Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuazonic acid

Author(s):  Fa-zhong Yang, Bin Yang, Bei-bei Li, Chun Xiao

Affiliation(s):  School of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; more

Corresponding email(s):   x.chun@163.com

Key Words:  Toxin, Induced resistance, Fungus-plant-insect system, Plant-mediated interaction, Tenuazonic acid, Alternaria alternata, Macrosiphum rosivorum

Fa-zhong Yang, Bin Yang, Bei-bei Li, Chun Xiao. Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuazonic acid[J]. Journal of Zhejiang University Science B, 2015, 16(4): 264-274.

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author="Fa-zhong Yang, Bin Yang, Bei-bei Li, Chun Xiao",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuazonic acid
%A Fa-zhong Yang
%A Bin Yang
%A Bei-bei Li
%A Chun Xiao
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 4
%P 264-274
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400151

T1 - Alternaria toxin-induced resistance in rose plants against rose aphid (Macrosiphum rosivorum): effect of tenuazonic acid
A1 - Fa-zhong Yang
A1 - Bin Yang
A1 - Bei-bei Li
A1 - Chun Xiao
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 4
SP - 264
EP - 274
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400151

Many different types of toxins are produced by the fungus, Alternaria alternata (Fr.) Keissler. Little is known, however, regarding the influence of these toxins on insects. In this study, we investigated the toxin-induced inhibitory effects of the toxin produced by A. alternata on the rose aphid, Macrosiphum rosivorum, when the toxin was applied to leaves of the rose, Rosa chinensis. The results demonstrated that the purified crude toxin was non-harmful to rose plants and rose aphids, but had an intensive inhibitory effect on the multiplication of aphids. The inhibitory index against rose aphids reached 87.99% when rose plants were sprayed with the toxin solution at a low concentration. Further results from bioassays with aphids and high performance liquid chromatography (HPLC) analyses demonstrated that tenuazonic acid (TeA) was one of the most important resistance-related active components in the crude toxin. The content of TeA was 0.1199% in the crude toxin under the HPLC method. Similar to the crude toxin, the inhibitory index of pure TeA reached 83.60% 15 d after the rose plants were sprayed with pure TeA solution at the lower concentration of 0.060 μg/ml, while the contents of residual TeA on the surface and in the inner portion of the rose plants were only 0.04 and 0.00 ng/g fresh weight of TeA-treated rose twigs, respectively, 7 d after the treatment. Our results show that TeA, an active component in the A. alternata toxin, can induce the indirect plant-mediated responses in rose plants to intensively enhance the plant’s resistances against rose aphids, and the results are very helpful to understand the plant-mediated interaction between fungi and insects on their shared host plants.


结论:(1)链格孢菌粗毒素和对照品TeA均能使中国月季植株产生对月季长管蚜的系统诱导抗性,显 著降低月季长管蚜对中国月季的危害;(2)链格孢菌粗毒素中的主要抗蚜活性成分是TeA,TeA有望成为中国月季上具有抗蚜活性的先导化合物;(3)粗毒素和对照品TeA对蚜虫和植物均无伤害作用,但能激活植物对虫害的诱导抗性(ISR)和系统获得性抗性(SAR),可直接证实二者间存在着寄主植物介导的间接的病虫互作关系。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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