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CLC number: R394-3

On-line Access: 2015-10-03

Received: 2014-11-19

Revision Accepted: 2015-06-08

Crosschecked: 2015-07-09

Cited: 1

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Citations:  Bibtex RefMan EndNote GB/T7714


Jing Jiang


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.10 P.845-856


Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit

Author(s):  Ning Zhang, Jing Jiang, Yan-li Yang, Zhi-he Wang

Affiliation(s):  Key Laboratory of Protected Horticulture, Ministry of Education, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding email(s):   zhangningsyau@163.com, jj_syau@hotmail.com

Key Words:  Invertase inhibitor, Fruit development, Transient transformation system, Solanum lycopersicum, Overexpression

Ning Zhang, Jing Jiang, Yan-li Yang, Zhi-he Wang. Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit[J]. Journal of Zhejiang University Science B, 2015, 16(10): 845-856.

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%T Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit
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T1 - Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit
A1 - Ning Zhang
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400319

In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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