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CLC number: R697+.22

On-line Access: 2016-01-06

Received: 2015-07-16

Revision Accepted: 2015-11-09

Crosschecked: 2015-12-10

Cited: 3

Clicked: 2117

Citations:  Bibtex RefMan EndNote GB/T7714


Supatcharee Arun


Sitthichai Iamsaard


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.1 P.21-29


Changes of testicular phosphorylated proteins in response to restraint stress in male rats

Author(s):  Supatcharee Arun, Jaturon Burawat, Wannisa Sukhorum, Apichakan Sampannang, Nongnut Uabundit, Sitthichai Iamsaard

Affiliation(s):  1Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; more

Corresponding email(s):   sittia@kku.ac.th

Key Words:  Restraint-stress rats, Steroidogenic acute regulatory (StAR) protein, Testicular phosphorylated protein

Supatcharee Arun, Jaturon Burawat, Wannisa Sukhorum, Apichakan Sampannang, Nongnut Uabundit, Sitthichai Iamsaard. Changes of testicular phosphorylated proteins in response to restraint stress in male rats[J]. Journal of Zhejiang University Science B, 2016, 17(1): 21-29.

@article{title="Changes of testicular phosphorylated proteins in response to restraint stress in male rats",
author="Supatcharee Arun, Jaturon Burawat, Wannisa Sukhorum, Apichakan Sampannang, Nongnut Uabundit, Sitthichai Iamsaard",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Changes of testicular phosphorylated proteins in response to restraint stress in male rats
%A Supatcharee Arun
%A Jaturon Burawat
%A Wannisa Sukhorum
%A Apichakan Sampannang
%A Nongnut Uabundit
%A Sitthichai Iamsaard
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 1
%P 21-29
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500174

T1 - Changes of testicular phosphorylated proteins in response to restraint stress in male rats
A1 - Supatcharee Arun
A1 - Jaturon Burawat
A1 - Wannisa Sukhorum
A1 - Apichakan Sampannang
A1 - Nongnut Uabundit
A1 - Sitthichai Iamsaard
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 1
SP - 21
EP - 29
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500174

Objective: To investigate male reproductive parameters via changes of potential testicular protein markers in restraint-stress rats. Methods: Male Sprague-Dawley rats were divided into two groups (non-immobilized control and restraint-immobilized/stress groups, n=8 each group). The stress animals were immobilized (12 h/d) by a restraint cage for 7 consecutive days. All reproductive parameters, morphology and histology were observed and compared between groups. In addition, the expression of steroidogenic acute regulatory (StAR) and phosphotyrosine proteins (previously localized in Sertoli and late spermatid cells) in testicular lysate was assayed by immuno-Western blotting. Results: Testosterone level, sperm concentration and sperm head normality of stress rats were significantly decreased while the corticosterone level was increased as compared with the control (P<0.05). Histologically, stress rats showed low sperm mass in epididymal lumen and some atrophy of seminiferous tubules. Although the expression of testicular StAR protein was not significantly different between groups, changed patterns of the 131, 95, and 75 kDa testicular phosphorylated proteins were observed in the stress group compared with the control group. The intensity of a testicular 95-kDa phosphorylated protein was significantly decreased in stress rats. Conclusions: This study has demonstrated the alteration of testicular phosphorylated protein patterns, associated with adverse male reproductive parameters in stress rats. It could be an explanation of some infertility in stress males.


结果:实验组大鼠的睾酮水平和精子密度较对照组均显著降低,而精子畸形率和皮质酮显著升高。组织学比较发现实验组大鼠精子团数量减少并且出现输精管萎缩。两组大鼠睾丸组织中酪氨酸磷酸化蛋白的表达具有显著差异,实验组95 kDa的磷酸化蛋白表达量显著降低,但两组急性类固醇调节蛋白的表达没有显著差异。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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