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CLC number: R589.2

On-line Access: 2016-06-03

Received: 2016-02-19

Revision Accepted: 2016-04-28

Crosschecked: 2016-05-12

Cited: 4

Clicked: 2044

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Wei Chen

http://orcid.org/0000-0002-2373-2437

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.6 P.437-446

http://doi.org/10.1631/jzus.B1600074


Myricetin protects against diet-induced obesity and ameliorates oxidative stress in C57BL/6 mice


Author(s):  Hong-ming Su, Li-na Feng, Xiao-dong Zheng, Wei Chen

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   zjuchenwei@zju.edu.cn

Key Words:  Myricetin, Obesity, Adipogenesis, Oxidative stress


Hong-ming Su, Li-na Feng, Xiao-dong Zheng, Wei Chen. Myricetin protects against diet-induced obesity and ameliorates oxidative stress in C57BL/6 mice[J]. Journal of Zhejiang University Science B, 2016, 17(6): 437-446.

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author="Hong-ming Su, Li-na Feng, Xiao-dong Zheng, Wei Chen",
journal="Journal of Zhejiang University Science B",
volume="17",
number="6",
pages="437-446",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600074"
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%T Myricetin protects against diet-induced obesity and ameliorates oxidative stress in C57BL/6 mice
%A Hong-ming Su
%A Li-na Feng
%A Xiao-dong Zheng
%A Wei Chen
%J Journal of Zhejiang University SCIENCE B
%V 17
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%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600074

TY - JOUR
T1 - Myricetin protects against diet-induced obesity and ameliorates oxidative stress in C57BL/6 mice
A1 - Hong-ming Su
A1 - Li-na Feng
A1 - Xiao-dong Zheng
A1 - Wei Chen
J0 - Journal of Zhejiang University Science B
VL - 17
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SP - 437
EP - 446
%@ 1673-1581
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600074


Abstract: 
Background: myricetin is a naturally occurring antioxidant commonly found in various plants. However, little information is available with respect to its direct anti-obesity effects. Objective: This study was undertaken to investigate the effect of myricetin on high-fat diet (HFD)-induced obesity in C57BL/6 mice. Results: Administration of myricetin dramatically reduced the body weight of diet-induced obese mice compared with solely HFD-induced mice. Several parameters related to obesity including serum glucose, triglyceride, and cholesterol were significantly decreased in myricetin-treated mice. Moreover, obesity-associated oxidative stress (glutathione peroxidase (GPX) activity, total antioxidant capacity (T-AOC), and malondialdehyde (MDA)) and inflammation (tumor necrosis factor-α (TNF-α)) were ameliorated in myricetin-treated mice. Further investigation revealed that the protective effect of myricetin against HFD-induced obesity in mice appeared to be partially mediated through the down-regulation of mRNA expression of adipogenic transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), and lipogenic transcription factor sterol regulatory element-binding protein 1c (SREBP-1c). Conclusions: Consumption of myricetin may help to prevent obesity and obesity-related metabolic complications.

杨梅素对膳食诱导的C57BL/6小鼠肥胖及氧化应激的干预作用

目的:研究杨梅素(myricetin)对高脂膳食(HFD)诱导的C57BL/6小鼠肥胖的干预作用,并探讨其作用机理。
创新点:首次在高脂膳食诱导的C57BL/6肥胖小鼠模型中证明杨梅素可明显减轻肥胖小鼠的体重,同时改善伴随肥胖的氧化应激和炎症。分子机理研究表明杨梅素通过下调脂代谢相关的转录因子(PPARγC/EBPαSREBP-1c)mRNA的表达,从而发挥减肥功效。
方法:将C57BL/6小鼠分为正常膳食组(ND,n=12)和高脂膳食组(HFD,n=24),分别给予正常膳食和高脂膳食喂食2周。之后,高脂膳食组分为高脂膳食组(n=12)和杨梅素保护组(HFD+M,灌胃150 mg/(kg·d)的杨梅素,n=12),继续处理10周。分析小鼠常规生理指标(体重和脏器重量)、生化指标(血糖浓度、甘油三酯和总胆固醇含量)、氧化应激指标(谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC))和炎症指标(肿瘤坏死因子α(TNF-α))等。苏木精-伊红染色法(H&E)和油红染色法分别观察脂肪组织和肝脏组织形态。实时逆转录聚合酶链反应(RT-PCR)分析小鼠脂肪组织PPARγC/EBPαSREBP-1c等基因的mRNA表达水平。
结论:与高脂膳食对照组相比,杨梅素处理可以显著减轻肥胖小鼠的体重。同时能显著降低肥胖小鼠血糖、甘油三酯和总胆固醇的含量。此外,杨梅素处理能缓解伴随肥胖的氧化应激(GPX活性、T-AOC和丙二醛(MDA))和炎症(TNF-α)。分子机理研究发现杨梅素通过下调脂肪细胞生成(PPARγC/EBPα)和脂质合成(SREBP-1c)密切相关的转录因子的mRNA表达。本研究结果将为杨梅素减肥产品的开发提供一定的理论依据。

关键词:杨梅素;肥胖;脂肪生成;氧化应激

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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