CLC number: R318.08
On-line Access: 2017-11-06
Received: 2016-09-29
Revision Accepted: 2017-02-20
Crosschecked: 2017-10-20
Cited: 0
Clicked: 5741
Citations: Bibtex RefMan EndNote GB/T7714
Bing Zhang, Pei-biao Zhang, Zong-liang Wang, Zhong-wen Lyu, Han Wu. Tissue-engineered composite scaffold of poly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded with autologous mesenchymal stem cells for bone regeneration[J]. Journal of Zhejiang University Science B, 2017, 18(11): 963-976.
@article{title="Tissue-engineered composite scaffold of poly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded with autologous mesenchymal stem cells for bone regeneration",
author="Bing Zhang, Pei-biao Zhang, Zong-liang Wang, Zhong-wen Lyu, Han Wu",
journal="Journal of Zhejiang University Science B",
volume="18",
number="11",
pages="963-976",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600412"
}
%0 Journal Article
%T Tissue-engineered composite scaffold of poly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded with autologous mesenchymal stem cells for bone regeneration
%A Bing Zhang
%A Pei-biao Zhang
%A Zong-liang Wang
%A Zhong-wen Lyu
%A Han Wu
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 11
%P 963-976
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600412
TY - JOUR
T1 - Tissue-engineered composite scaffold of poly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded with autologous mesenchymal stem cells for bone regeneration
A1 - Bing Zhang
A1 - Pei-biao Zhang
A1 - Zong-liang Wang
A1 - Zhong-wen Lyu
A1 - Han Wu
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 11
SP - 963
EP - 976
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600412
Abstract: Objective: A new therapeutic strategy using nanocomposite scaffolds of grafted hydroxyapatite (g-HA)/poly(lactide-co-glycolide) (PLGA) carried with autologous mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2) was assessed for the therapy of critical bone defects. At the same time, tissue response and in vivo mineralization of tissue-engineered implants were investigated. Methods: A composite scaffold of PLGA and g-HA was fabricated by the solvent casting and particulate-leaching method. The tissue-engineered implants were prepared by seeding the scaffolds with autologous bone marrow MSCs in vitro. Then, mineralization and osteogenesis were observed by intramuscular implantation, as well as the repair of the critical radius defects in rabbits. Results: After eight weeks post-surgery, scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) revealed that g-HA/PLGA had a better interface of tissue response and higher mineralization than PLGA. Apatite particles were formed and varied both in macropores and micropores of g-HA/PLGA. Computer radiographs and histological analysis revealed that there were more and more quickly formed new bone formations and better fusion in the bone defect areas of g-HA/PLGA at 2–8 weeks post-surgery. Typical bone synostosis between the implant and bone tissue was found in g-HA/PLGA, while only fibrous tissues formed in PLGA. Conclusions: The incorporation of g-HA mainly improved mineralization and bone formation compared with PLGA. The application of MSCs can enhance bone formation and mineralization in PLGA scaffolds compared with cell-free scaffolds. Furthermore, it can accelerate the absorption of scaffolds compared with composite scaffolds.
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