CLC number: S855.3
On-line Access: 2018-07-04
Received: 2017-06-16
Revision Accepted: 2017-10-01
Crosschecked: 2018-06-08
Cited: 0
Clicked: 4949
Ying Shan, Zi-Qi Liu, Guo-Wei Li, Cong Chen, Hao Luo, Ya-Jie Liu, Xun-Hui Zhuo, Xing-Fen Shi, Wei-Huan Fang, Xiao-Liang Li. Nucleocapsid protein from porcine epidemic diarrhea virus isolates can antagonize interferon-λ production by blocking the nuclear factor-κB nuclear translocation[J]. Journal of Zhejiang University Science B, 2018, 19(7): 570-580.
@article{title="Nucleocapsid protein from porcine epidemic diarrhea virus isolates can antagonize interferon-λ production by blocking the nuclear factor-κB nuclear translocation",
author="Ying Shan, Zi-Qi Liu, Guo-Wei Li, Cong Chen, Hao Luo, Ya-Jie Liu, Xun-Hui Zhuo, Xing-Fen Shi, Wei-Huan Fang, Xiao-Liang Li",
journal="Journal of Zhejiang University Science B",
volume="19",
number="7",
pages="570-580",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700283"
}
%0 Journal Article
%T Nucleocapsid protein from porcine epidemic diarrhea virus isolates can antagonize interferon-λ production by blocking the nuclear factor-κB nuclear translocation
%A Ying Shan
%A Zi-Qi Liu
%A Guo-Wei Li
%A Cong Chen
%A Hao Luo
%A Ya-Jie Liu
%A Xun-Hui Zhuo
%A Xing-Fen Shi
%A Wei-Huan Fang
%A Xiao-Liang Li
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 7
%P 570-580
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700283
TY - JOUR
T1 - Nucleocapsid protein from porcine epidemic diarrhea virus isolates can antagonize interferon-λ production by blocking the nuclear factor-κB nuclear translocation
A1 - Ying Shan
A1 - Zi-Qi Liu
A1 - Guo-Wei Li
A1 - Cong Chen
A1 - Hao Luo
A1 - Ya-Jie Liu
A1 - Xun-Hui Zhuo
A1 - Xing-Fen Shi
A1 - Wei-Huan Fang
A1 - Xiao-Liang Li
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 7
SP - 570
EP - 580
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700283
Abstract: porcine epidemic diarrhea virus (PEDV) is a highly infectious pathogen that can cause severe diseases in pigs and result in enormous economic losses in the worldwide swine industry. Previous studies revealed that PEDV exhibits an obvious capacity for modulating interferon (IFN) signaling or expression. The newly discovered type III IFN, which plays a crucial role in antiviral immunity, has strong antiviral activity against PEDV proliferation in IPEC-J2 cells. In this study, we aimed to investigate the effect of PEDV nucleocapsid (N) protein on type III IFN-λ. We found that the N proteins of ten PEDV strains isolated between 2013 and 2017 from different local farms shared high nucleotide identities, while the N protein of the CV777 vaccine strain formed a monophyletic branch in the phylogenetic tree. The N protein of the epidemic strain could antagonize type III IFN, but not type I or type II IFN expression induced by polyinosinic-polycytidylic acid (poly(I:C)) in IPEC-J2 cells. Subsequently, we demonstrated that the inhibition of poly(I:C)-induced IFN-λ3 production by PEDV N protein was dependent on the blocking of nuclear factor-κ;b (NF-κ;B) nuclear translocation. These findings might help increase understanding of the pathogenesis of PEDV and its mechanisms for evading the host immune response.
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