CLC number: TS207.5
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2018-10-10
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Xian Zhang, Ke He, Yun Fang, Tong Cao, Narayan Paudyal, Xiao-feng Zhang, Hou-hui Song, Xiao-liang Li, Wei-huan Fang. Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples[J]. Journal of Zhejiang University Science B, 2018, 19(11): 871-883.
@article{title="Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples",
author="Xian Zhang, Ke He, Yun Fang, Tong Cao, Narayan Paudyal, Xiao-feng Zhang, Hou-hui Song, Xiao-liang Li, Wei-huan Fang",
journal="Journal of Zhejiang University Science B",
volume="19",
number="11",
pages="871-883",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800085"
}
%0 Journal Article
%T Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples
%A Xian Zhang
%A Ke He
%A Yun Fang
%A Tong Cao
%A Narayan Paudyal
%A Xiao-feng Zhang
%A Hou-hui Song
%A Xiao-liang Li
%A Wei-huan Fang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 11
%P 871-883
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800085
TY - JOUR
T1 - Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples
A1 - Xian Zhang
A1 - Ke He
A1 - Yun Fang
A1 - Tong Cao
A1 - Narayan Paudyal
A1 - Xiao-feng Zhang
A1 - Hou-hui Song
A1 - Xiao-liang Li
A1 - Wei-huan Fang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 11
SP - 871
EP - 883
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800085
Abstract: A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.
[1]Alshannaq A, Yu JH, 2017. Occurrence, toxicity, and analysis of major mycotoxins in food. Int J Environ Res Public Health, 14(6):632.
[2]Anfossi L, Giovannoli C, Giraudi G, et al., 2012. A lateral flow immunoassay for the rapid detection of ochratoxin A in wine and grape must. J Agric Food Chem, 60(46):11491-11497.
[3]Asghar MA, Iqbal J, Ahmed A, et al., 2016. Development and validation of a high-performance liquid chromatography method with post-column derivatization for the detection of aflatoxins in cereals and grains. Toxicol Ind Health, 32(6):1122-1134.
[4]Bernhardt K, Valenta H, Kersten S, et al., 2016. Determination of T-2 toxin, HT-2 toxin, and three other type A trichothecenes in layer feed by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS)— comparison of two sample preparation methods. Mycotoxin Res, 32(2):89-97.
[5]Bienenmann-Ploum ME, Vincent U, Campbell K, et al., 2013. Single-laboratory validation of a multiplex flow cytometric immunoassay for the simultaneous detection of coccidiostats in eggs and feed. Anal Bioanal Chem, 405(29):9571-9577.
[6]Burkin AA, Kononenko GP, Soboleva NA, 2002. Group-specific antibodies against zearalenone and its metabolites and synthetic analogs. Appl Biochem Microbiol, 38(2):169-176.
[7]Burmistrova NA, Goryacheva IY, Basova EY, et al., 2009. Application of a new anti-zearalenone monoclonal antibody in different immunoassay formats. Anal Bioanal Chem, 395(5):1301-1307.
[8]Byzova NA, Zvereva EA, Zherdev AV, et al., 2010. Rapid pretreatment-free immunochromatographic assay of chloramphenicol in milk. Talanta, 81(3):843-848.
[9]Cheat S, Pinton P, Cossalter AM, et al., 2016. The mycotoxins deoxynivalenol and nivalenol show in vivo synergism on jejunum enterocytes apoptosis. Food Chem Toxicol, 87: 45-54.
[10]Cho YJ, Lee DH, Kim DO, et al., 2005. Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay. J Agric Food Chem, 53(22):8447-8451.
[11]Chun HS, Choi EH, Chang HJ, et al., 2009. A fluorescence polarization immunoassay for the detection of zearalenone in corn. Anal Chim Acta, 639(1-2):83-89.
[12]de Lima Rocha DF, dos Santos Oliveira M, Furlong EB, et al., 2017. Evaluation of the TLC quantification method and occurrence of deoxynivalenol in wheat flour of southern Brazil. Food Addit Contam Part A, 34(12):2220-2229.
[13]Frens G, 1973. Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nat Phys Sci, 241(105):20-22.
[14]Gendloff EH, Casale WL, Ram BP, et al., 1986. Hapten- protein conjugates prepared by the mixed anhydride method: cross-reactive antibodies in heterologous antisera. J Immunol Methods, 92(1):15-20.
[15]Guo YR, Liu SY, Gui WJ, et al., 2009. Gold immunochromatographic assay for simultaneous detection of carbofuran and triazophos in water samples. Anal Biochem, 389(1):32-39.
[16]Huang ZB, Xu Y, Li LS, et al., 2012. Development of an immunochromatographic strip test for the rapid simultaneous detection of deoxynivalenol and zearalenone in wheat and maize. Food Control, 28(1):7-12.
[17]Ji F, Mokoena MP, Zhao HY, et al., 2017. Development of an immunochromatographic strip test for the rapid detection of zearalenone in wheat from Jiangsu province, China. PLoS ONE, 12(5):e0175282.
[18]Kawamura O, Sato S, Kajii H, et al., 1989. A sensitive enzyme-linked immunosorbent assay of ochratoxin A based on monoclonal antibodies. Toxicon, 27(8):887-897.
[19]Kolosova AY, de Saeger S, Sibanda L, et al., 2007. Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol. Anal Bioanal Chem, 389(7-8):2103-2107.
[20]Lee HJ, Ryu D, 2017. Worldwide occurrence of mycotoxins in cereals and cereal-derived food products: public health perspectives of their co-occurrence. J Agric Food Chem, 65(33):7034-7051.
[21]Li X, Li PW, Zhang Q, et al., 2013. Multi-component immunochromatographic assay for simultaneous detection of aflatoxin B1, ochratoxin A and zearalenone in agro-food. Biosens Bioelectron, 49:426-432.
[22]Liu DW, Liu HY, Zhang HB, et al., 2016. Potential natural exposure of endangered red-crowned crane (Grus japonensis) to mycotoxins aflatoxin B1, deoxynivalenol, zearalenone, T-2 toxin, and ochratoxin A. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 17(2):158-168.
[23]Liu H, Xiu Y, Xu Y, et al., 2017. Development of a colloidal gold immunochromatographic assay (GICA) for the rapid detection of Spiroplasma eriocheiris in commercially exploited crustaceans from China. J Fish Dis, 40(12):1839-1847.
[24]Liu R, Liu Y, Lan MJ, et al., 2016. Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 17(4):282-293.
[25]Long M, Yang SH, Wang Y, et al., 2016. The protective effect of selenium on chronic zearalenone-induced reproductive system damage in male mice. Molecules, 21(12):1687.
[26]Luo ME, Tang Y, Xiang JJ, et al., 2013. Preparation of anti-zearalenone monoclonal antibody and preliminary establishment of colloidal gold immunochromatographic assay for zearalenone. Chin J Cell Mol Immunol, 29(7):729-733 (in Chinese).
[27]Majdinasab M, Sheikh-Zeinoddin M, Soleimanian-Zad S, et al., 2015. Ultrasensitive and quantitative gold nanoparticle-based immunochromatographic assay for detection of ochratoxin A in agro-products. J Chromatogr B, 974: 147-154.
[28]Molinelli A, Grossalber K, Krska R, 2009. A rapid lateral flow test for the determination of total type B fumonisins in maize. Anal Bioanal Chem, 395(5):1309-1316.
[29]Perry JL, Christensen T, Goldsmith MR, et al., 2003. Binding of ochratoxin A to human serum albumin stabilized by a protein-ligand ion pair. J Phys Chem B, 107(31):7884-7888.
[30]Pierron A, Alassane-Kpembi I, Oswald IP, 2016. Impact of mycotoxin on immune response and consequences for pig health. Anim Nutr, 2(2):63-68.
[31]Schmidt-Heydt M, Geisen R, 2007. A microarray for monitoring the production of mycotoxins in food. Int J Food Microbiol, 117(2):131-140.
[32]Shim WB, Kim KY, Chung DH, 2009a. Development and validation of a gold nanoparticle immunochromatographic assay (ICG) for the detection of zearalenone. J Agric Food Chem, 57(10):4035-4041.
[33]Shim WB, Dzantiev BB, Eremin SA, et al., 2009b. One-step simultaneous immunochromatographic strip test for multianalysis of ochratoxin a and zearalenone. J Microbiol Biotechnol, 19(1):83-92.
[34]Song CM, Liu QT, Zhi AM, et al., 2011. Development of a lateral flow colloidal gold immunoassay strip for the rapid detection of olaquindox residues. J Agric Food Chem, 59(17):9319-9326.
[35]Song G, Wu JY, Xie Y, et al., 2017. Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 18(12):1075-1082.
[36]Sun SJ, Xie J, Peng T, et al., 2017. Broad-spectrum immunoaffinity cleanup for the determination of aflatoxins B1, B2, G1, G2, M1, M2 in Ophiocordyceps sinensis and its pharmaceutical preparations by ultra performance liquid chromatography tandem mass spectrometry. J Chromatogr B, 1068-1069:112-118.
[37]Sun YB, Hu XF, Zhang Y, et al., 2014. Development of an immunochromatographic strip test for the rapid detection of zearalenone in corn. J Agric Food Chem, 62(46):11116-11121.
[38]Sun YN, Xing GX, Yang JF, et al., 2016. Development of an immunochromatographic test strip for simultaneous qualitative and quantitative detection of ochratoxin A and zearalenone in cereal. J Sci Food Agric, 96(11):3673-3678.
[39]Torović L, 2018. Aflatoxins and ochratoxin A in flour: a survey of the Serbian retail market. Food Addit Contam Part B Surveill, 11(1):26-32.
[40]Urusov AE, Petrakova AV, Gubaydullina MK, et al., 2017. High-sensitivity immunochromatographic assay for fumonisin B1 based on indirect antibody labeling. Biotechnol Lett, 39(5):751-758.
[41]Wang XC, Fan HX, Fan MX, et al., 2016. A sensitive immunochromatographic assay using colloidal gold-antibody probe for rapid detection of fumonisin B1 in corn. Food Addit Contam Part A, 33(9):1435-1443.
[42]Wang Y, Liu N, Ning BN, et al., 2012. Simultaneous and rapid detection of six different mycotoxins using an immunochip. Biosens Bioelectron, 34(1):44-50.
[43]Wang YK, Yan YX, Ji WH, et al., 2013a. Novel chemiluminescence immunoassay for the determination of zearalenone in food samples using gold nanoparticles labeled with streptavidin-horseradish peroxidase. J Agric Food Chem, 61(18):4250-4256.
[44]Wang YK, Yan YX, Ji WH, et al., 2013b. Rapid simultaneous quantification of zearalenone and fumonisin B1 in corn and wheat by lateral flow dual immunoassay. J Agric Food Chem, 61(21):5031-5036.
[45]Wu JX, Zhang SE, Zhou XP, 2010. Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 11(1):52-60.
[46]Yan QH, Zhou JX, Li HZ, et al., 2015. Coexistence of and interaction relationships between an aflatoxin-producing fungus and a bacterium. Fungal Biol, 119(7):605-614.
[47]Yang C, Lates V, Prieto-Simón B, et al., 2012. Aptamer-DNAzyme hairpins for biosensing of ochratoxin A. Biosens Bioelectron, 32(1):208-212.
[48]Yang SP, Zhang HY, Sun FF, et al., 2017. Metabolic profile of zearalenone in liver microsomes from different species and its in vivo metabolism in rats and chickens using ultra high-pressure liquid chromatography-quadrupole/time-of-flight mass spectrometry. J Agric Food Chem, 65(51):11292-11303.
[49]Zhang MY, Yan LZ, Huang Q, et al., 2018. Highly sensitive simultaneous detection of major ochratoxins by an immunochromatographic assay. Food Control, 84:215-220.
[50]Zhang X, Sun MJ, Kang Y, et al., 2015a. Identification of a high-affinity monoclonal antibody against ochratoxin A and its application in enzyme-linked immunosorbent assay. Toxicon, 106:89-96.
[51]Zhang X, Wang X, Sun MJ, et al., 2015b. A magnetic nanoparticle based enzyme-linked immunosorbent assay for sensitive quantification of zearalenone in cereal and feed samples. Toxins, 7(10):4216-4231.
[52]List of electronic supplementary materials
[53]Table S1 Optimal conditions of the dual flow immunochromatographic assay
[54]Table S2 Buffer types, optimum buffers, and optimum concentrations of additives in the dual flow immunochromatographic assay
[55]Table S3 Comparison of results of gold nanoparticles-based immunochromatographic assay published in the past
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