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Abstract: A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.

赭曲霉毒素A和玉米赤霉烯酮二联定量胶体金免疫层析试纸条的制备及应用

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[52]List of electronic supplementary materials

[53]Table S1 Optimal conditions of the dual flow immunochromatographic assay

[54]Table S2 Buffer types, optimum buffers, and optimum concentrations of additives in the dual flow immunochromatographic assay

[55]Table S3 Comparison of results of gold nanoparticles-based immunochromatographic assay published in the past