CLC number: TS207.5
On-line Access: 2018-11-02
Received: 2018-03-06
Revision Accepted: 2018-07-31
Crosschecked: 2018-10-10
Cited: 0
Clicked: 4211
Xian Zhang, Ke He, Yun Fang, Tong Cao, Narayan Paudyal, Xiao-feng Zhang, Hou-hui Song, Xiao-liang Li, Wei-huan Fang. Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples[J]. Journal of Zhejiang University Science B, 2018, 19(11): 871-883.
@article{title="Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples",
author="Xian Zhang, Ke He, Yun Fang, Tong Cao, Narayan Paudyal, Xiao-feng Zhang, Hou-hui Song, Xiao-liang Li, Wei-huan Fang",
journal="Journal of Zhejiang University Science B",
volume="19",
number="11",
pages="871-883",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800085"
}
%0 Journal Article
%T Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples
%A Xian Zhang
%A Ke He
%A Yun Fang
%A Tong Cao
%A Narayan Paudyal
%A Xiao-feng Zhang
%A Hou-hui Song
%A Xiao-liang Li
%A Wei-huan Fang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 11
%P 871-883
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800085
TY - JOUR
T1 - Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples
A1 - Xian Zhang
A1 - Ke He
A1 - Yun Fang
A1 - Tong Cao
A1 - Narayan Paudyal
A1 - Xiao-feng Zhang
A1 - Hou-hui Song
A1 - Xiao-liang Li
A1 - Wei-huan Fang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 11
SP - 871
EP - 883
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800085
Abstract: A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.
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[52]List of electronic supplementary materials
[53]Table S1 Optimal conditions of the dual flow immunochromatographic assay
[54]Table S2 Buffer types, optimum buffers, and optimum concentrations of additives in the dual flow immunochromatographic assay
[55]Table S3 Comparison of results of gold nanoparticles-based immunochromatographic assay published in the past
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