CLC number: S855.1
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2019-06-13
Cited: 0
Clicked: 3418
Ke He, Yong-Ping Xin, Ying Shan, Xian Zhang, Hou-Hui Song, Wei-Huan Fang. Phosphorylation residue T175 in RsbR protein is required for efficient induction of sigma B factor and survival of Listeria monocytogenes under acidic stress[J]. Journal of Zhejiang University Science B, 2019, 20(8): 660-669.
@article{title="Phosphorylation residue T175 in RsbR protein is required for efficient induction of sigma B factor and survival of Listeria monocytogenes under acidic stress",
author="Ke He, Yong-Ping Xin, Ying Shan, Xian Zhang, Hou-Hui Song, Wei-Huan Fang",
journal="Journal of Zhejiang University Science B",
volume="20",
number="8",
pages="660-669",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800551"
}
%0 Journal Article
%T Phosphorylation residue T175 in RsbR protein is required for efficient induction of sigma B factor and survival of Listeria monocytogenes under acidic stress
%A Ke He
%A Yong-Ping Xin
%A Ying Shan
%A Xian Zhang
%A Hou-Hui Song
%A Wei-Huan Fang
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 8
%P 660-669
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800551
TY - JOUR
T1 - Phosphorylation residue T175 in RsbR protein is required for efficient induction of sigma B factor and survival of Listeria monocytogenes under acidic stress
A1 - Ke He
A1 - Yong-Ping Xin
A1 - Ying Shan
A1 - Xian Zhang
A1 - Hou-Hui Song
A1 - Wei-Huan Fang
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 8
SP - 660
EP - 669
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800551
Abstract: Listeria monocytogenes is an important zoonotic foodborne pathogen that can tolerate a number of environmental stresses. rsbR, an upstream regulator of the sigma B (SigB) factor, is thought to sense environmental challenges and trigger the SigB pathway. In Bacillus subtilis, two phosphorylation sites in rsbR are involved in activating the SigB pathway and a feedback mechanism, respectively. In this study, the role of rsbR in L. monocytogenes under mild and severe stresses was investigated. Strains with genetic deletion (ΔrsbR), complementation (C-ΔrsbR), and phosphorylation site mutations in the rsbR (rsbR-T175A, rsbR-T209A, and rsbR-T175A-T209A) were constructed to evaluate the roles of these rsbR sequences in listerial growth and survival. SigB was examined at the transcriptional and translational levels. Deletion of rsbR reduced listerial growxth and survival in response to acidic stress. Substitution of the phosphorylation residue rsbR-T175A disabled rsbR complementation, while rsbR-T209A significantly upregulated SigB expression and listerial survival. Our results provide clear evidence that two phosphorylation sites of rsbR are functional in L. monocytogenes under acidic conditions, similar to the situation in B. subtilis.
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