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CLC number: S432.1

On-line Access: 2019-08-05

Received: 2019-03-01

Revision Accepted: 2019-05-12

Crosschecked: 2019-07-09

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yan Liang

https://orcid.org/0000-0002-1248-8563

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.9 P.713-727

http://doi.org/10.1631/jzus.B1900105


Antepenultimate residue at the C-terminus of NADPH oxidase RBOHD is critical for its function in the production of reactive oxygen species in Arabidopsis


Author(s):  Qiu-Ying Li, Ping Li, Nang Myint Phyu Sin Htwe, Ke-Ke Shangguan, Yan Liang

Affiliation(s):  Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   yanliang@zju.edu.cn

Key Words:  Reactive oxygen species (ROS), NADPH oxidase (NOX), Microbe associated molecular pattern (MAMP), Lipopolysaccharides (LPS), Respiratory burst oxidase homolog D (RBOHD)


Qiu-Ying Li, Ping Li, Nang Myint Phyu Sin Htwe, Ke-Ke Shangguan, Yan Liang. Antepenultimate residue at the C-terminus of NADPH oxidase RBOHD is critical for its function in the production of reactive oxygen species in Arabidopsis[J]. Journal of Zhejiang University Science B, 2019, 20(9): 713-727.

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author="Qiu-Ying Li, Ping Li, Nang Myint Phyu Sin Htwe, Ke-Ke Shangguan, Yan Liang",
journal="Journal of Zhejiang University Science B",
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number="9",
pages="713-727",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900105"
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%T Antepenultimate residue at the C-terminus of NADPH oxidase RBOHD is critical for its function in the production of reactive oxygen species in Arabidopsis
%A Qiu-Ying Li
%A Ping Li
%A Nang Myint Phyu Sin Htwe
%A Ke-Ke Shangguan
%A Yan Liang
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900105

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T1 - Antepenultimate residue at the C-terminus of NADPH oxidase RBOHD is critical for its function in the production of reactive oxygen species in Arabidopsis
A1 - Qiu-Ying Li
A1 - Ping Li
A1 - Nang Myint Phyu Sin Htwe
A1 - Ke-Ke Shangguan
A1 - Yan Liang
J0 - Journal of Zhejiang University Science B
VL - 20
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SP - 713
EP - 727
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1900105


Abstract: 
Production of reactive oxygen species (ROS) is a conserved immune response primarily mediated by NADPH oxidases (NOXs), also known in plants as respiratory burst oxidase homologs (RBOHs). Most microbe-associated molecular patterns (MAMPs) trigger a very fast and transient ROS burst in plants. However, recently, we found that lipopolysaccharides (LPS), a typical bacterial MAMP, triggered a biphasic ROS burst. In this study, we isolated mutants defective in LPS-triggered biphasic ROS burst (delt) in Arabidopsis, and cloned the DELT1 gene that was shown to encode RBOHD. In the delt1-2 allele, the antepenultimate residue, glutamic acid (E919), at the C-terminus of RBOHD was mutated to lysine (K). E919 is a highly conserved residue in NADPH oxidases, and a mutation of the corresponding residue E568 in human NOX2 has been reported to be one of the causes of chronic granulomatous disease. Consistently, we found that residue E919 was indispensable for RBOHD function in the MAMP-induced ROS burst and stomatal closure. It has been suggested that the mutation of this residue in other NADPH oxidases impairs the protein’s stability and complex assembly. However, we found that the E919K mutation did not affect RBOHD protein abundance or the ability of protein association, suggesting that the residue E919 in RBOHD might have a regulatory mechanism different from that of other NOXs. Taken together, our results confirm that the antepenultimate residue E is critical for NADPH oxidases and provide a new insight into the regulatory mechanisms of RBOHD.

拟南芥NADPH氧化酶RBOHD羧基端倒数第三位氨基酸在其介导活性氧迸发中的重要作用

目的:解析呼吸爆发氧化酶同系物蛋白D(RBOHD)介导活性氧迸发的分子机制.
创新点:首次研究RBOHD蛋白羧基端在植物体内活性氧迸发中的作用,并对其机制进行初步探究.
方法:本文利用正向遗传学方法筛选得到在多种病原物相关分子模式(PAMP)处理后活性氧不迸发的突变体delt.然后结合图位克隆和全基因测序技术,发现DELT1编码了RBOHD蛋白.DELT1-2在RBOHD羧基端倒数第三位谷氨酸位置发生了突变.深入分析发现,谷氨酸的突变不影响DELT1-2表达、蛋白定位和互作等功能,但会导致植物不响应PAMP诱导的气孔关闭.
结论:RBOHD羧基端倒数第三位谷氨酸对其功能发挥起着决定作用.

关键词:活性氧;NADPH氧化酶;微生物相关分子模式;脂多糖;呼吸爆发氧化酶同系物蛋白D(RBOHD)

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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