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CLC number: R779.65; Q819

On-line Access: 2019-11-21

Received: 2019-04-09

Revision Accepted: 2019-08-01

Crosschecked: 2019-10-11

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Liang Ma


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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.12 P.945-959


Integrated 3D bioprinting-based geometry-control strategy for fabricating corneal substitutes

Author(s):  Bin Zhang, Qian Xue, Han-Yi Hu, Meng-Fei Yu, Lei Gao, Yi-Chen Luo, Yang Li, Jin-Tao Li, Liang Ma, yu-Feng Yao, Hua-Yong Yang

Affiliation(s):  State Key Laboratory of Fluid Power & Mechatronic Systems, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   liangma@zju.edu.cn

Key Words:  3D bioprinting, Corneal alternative, Digital light processing (DLP), Extrusion, Geometry-control

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Bin Zhang, Qian Xue, Han-Yi Hu, Meng-Fei Yu, Lei Gao, Yi-Chen Luo, Yang Li, Jin-Tao Li, Liang Ma, yu-Feng Yao, Hua-Yong Yang. Integrated 3D bioprinting-based geometry-control strategy for fabricating corneal substitutes[J]. Journal of Zhejiang University Science B, 2019, 20(12): 945-959.

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author="Bin Zhang, Qian Xue, Han-Yi Hu, Meng-Fei Yu, Lei Gao, Yi-Chen Luo, Yang Li, Jin-Tao Li, Liang Ma, yu-Feng Yao, Hua-Yong Yang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Integrated 3D bioprinting-based geometry-control strategy for fabricating corneal substitutes
%A Bin Zhang
%A Qian Xue
%A Han-Yi Hu
%A Meng-Fei Yu
%A Lei Gao
%A Yi-Chen Luo
%A Yang Li
%A Jin-Tao Li
%A Liang Ma
%A yu-Feng Yao
%A Hua-Yong Yang
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 12
%P 945-959
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900190

T1 - Integrated 3D bioprinting-based geometry-control strategy for fabricating corneal substitutes
A1 - Bin Zhang
A1 - Qian Xue
A1 - Han-Yi Hu
A1 - Meng-Fei Yu
A1 - Lei Gao
A1 - Yi-Chen Luo
A1 - Yang Li
A1 - Jin-Tao Li
A1 - Liang Ma
A1 - yu-Feng Yao
A1 - Hua-Yong Yang
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 12
SP - 945
EP - 959
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1900190

Background: The shortage of donor corneas is a severe global issue, and hence the development of corneal alternatives is imperative and urgent. Although attempts to produce artificial cornea substitutes by tissue engineering have made some positive progress, many problems remain that hamper their clinical application worldwide. For example, the curvature of tissue-engineered cornea substitutes cannot be designed to fit the bulbus oculi of patients. Objective: To overcome these limitations, in this paper, we present a novel integrated three-dimensional (3D) bioprinting-based cornea substitute fabrication strategy to realize design, customized fabrication, and evaluation of multi-layer hollow structures with complicated surfaces. Methods: The key rationale for this method is to combine digital light processing (DLP) and extrusion bioprinting into an integrated 3D cornea bioprinting system. A designable and personalized corneal substitute was designed based on mathematical modelling and a computer tomography scan of a natural cornea. The printed corneal substitute was evaluated based on biomechanical analysis, weight, structural integrity, and fit. Results: The results revealed that the fabrication of high water content and highly transparent curved films with geometric features designed according to the natural human cornea can be achieved using a rapid, simple, and low-cost manufacturing process with a high repetition rate and quality. Conclusions: This study demonstrated the feasibility of customized design, analysis, and fabrication of a corneal substitute. The programmability of this method opens up the possibility of producing substitutes for other cornea-like shell structures with different scale and geometry features, such as the glomerulus, atrium, and oophoron.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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