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On-line Access: 2024-06-24

Received: 2023-05-08

Revision Accepted: 2023-08-09

Crosschecked: 2024-06-24

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Citations:  Bibtex RefMan EndNote GB/T7714


Miao CHU


Huaqiang TAO


Dechun GENG


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Journal of Zhejiang University SCIENCE B 2024 Vol.25 No.6 P.513-528


Heme oxygenase 1 linked to inactivation of subchondral osteoclasts in osteoarthritis

Author(s):  Miao CHU, Guangdong CHEN, Kai CHEN, Pengfei ZHU, Zhen WANG, Zhonglai QIAN, Huaqiang TAO, Yaozeng XU, Dechun GENG

Affiliation(s):  Department of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, China; more

Corresponding email(s):   szgengdc@suda.edu.cn, 20224032009@stu.suda.edu.cn

Key Words:  Osteoclast, Oxidative stress, Osteoarthritis (OA), Heme oxygenase 1 (HMOX1), Carnosol

Miao CHU, Guangdong CHEN, Kai CHEN, Pengfei ZHU, Zhen WANG, Zhonglai QIAN, Huaqiang TAO, Yaozeng XU, Dechun GENG. Heme oxygenase 1 linked to inactivation of subchondral osteoclasts in osteoarthritis[J]. Journal of Zhejiang University Science B, 2024, 25(6): 513-528.

@article{title="Heme oxygenase 1 linked to inactivation of subchondral osteoclasts in osteoarthritis",
author="Miao CHU, Guangdong CHEN, Kai CHEN, Pengfei ZHU, Zhen WANG, Zhonglai QIAN, Huaqiang TAO, Yaozeng XU, Dechun GENG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Heme oxygenase 1 linked to inactivation of subchondral osteoclasts in osteoarthritis
%A Miao CHU
%A Guangdong CHEN
%A Pengfei ZHU
%A Zhen WANG
%A Zhonglai QIAN
%A Huaqiang TAO
%A Yaozeng XU
%A Dechun GENG
%J Journal of Zhejiang University SCIENCE B
%V 25
%N 6
%P 513-528
%@ 1673-1581
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300303

T1 - Heme oxygenase 1 linked to inactivation of subchondral osteoclasts in osteoarthritis
A1 - Miao CHU
A1 - Guangdong CHEN
A1 - Kai CHEN
A1 - Pengfei ZHU
A1 - Zhen WANG
A1 - Zhonglai QIAN
A1 - Huaqiang TAO
A1 - Yaozeng XU
A1 - Dechun GENG
J0 - Journal of Zhejiang University Science B
VL - 25
IS - 6
SP - 513
EP - 528
%@ 1673-1581
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300303

osteoarthritis (OA) is a chronic progressive osteoarthropathy in the elderly. osteoclast activation plays a crucial role in the occurrence of subchondral bone loss in early OA. However, the specific mechanism of osteoclast differentiation in OA remains unclear. In our study, gene expression profiles related to OA disease progression and osteoclast activation were screened from the Gene Expression Omnibus (GEO) repository. GEO2R and Funrich analysis tools were employed to find differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses demonstrated that chemical carcinogenesis, reactive oxygen species (ROS), and response to oxidative stress were mainly involved in osteoclast differentiation in OA subchondral bone. Furthermore, fourteen DEGs that are associated with oxidative stress were identified. The first ranked differential gene, heme oxygenase 1 (HMOX1), was selected for further validation. Related results showed that osteoclast activation in the pathogenesis of OA subchondral bone is accompanied by the downregulation of HMOX1. carnosol was revealed to inhibit osteoclastogenesis by targeting HMOX1 and upregulating the expression of antioxidant protein in vitro. Meanwhile, carnosol was found to alleviate the severity of OA by inhibiting the activation of subchondral osteoclasts in vivo. Our research indicated that the activation of osteoclasts due to subchondral bone redox dysplasia may serve as a significant pathway for the advancement of OA. Targeting HMOX1 in subchondral osteoclasts may offer novel insights for the treatment of early OA.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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