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Bio-Design and Manufacturing  2021 Vol.4 No.3 P.641-674

http://doi.org/10.1007/s42242-021-00126-7


Recent advances in chemically defined and tunable hydrogel platforms for organoid culture


Author(s):  Tarun Agarwal, Nehar Celikkin, Marco Costantini, Tapas K. Maiti & Pooyan Makvandi

Affiliation(s):  Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India; more

Corresponding email(s):   tkmaiti@hijli.iitkgp.ernet.in, maititapask@gmail.com

Key Words:  Organoid, 3D culture, Biomaterials, Chemically defined hydrogels, Cell-instructive microenvironment


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Tarun Agarwal, Nehar Celikkin, Marco Costantini, Tapas K. Maiti & Pooyan Makvandi . Recent advances in chemically defined and tunable hydrogel platforms for organoid culture[J]. Journal of Zhejiang University Science D, 2021, 4(3): 641-674.

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Abstract: 
Recent developments in organoid culture technologies have made it possible to closely recapitulate intrinsic characteristics of different tissues under in vitro conditions. These organoids act as a translational bridge between the traditional 2D/3D cultures and the in vivo models for studying the tissue development processes, disease modeling, and drug screening. Matrigel and tissue-specific extracellular matrix have been shown to support organoid development, efficiently; however, their chemically undefined nature, non-tunable properties, and associated batch-to-batch variations often limit reproducibility of the assembly process. In this regard, chemically defined platforms offer wider opportunities to optimize and recreate tissue-specific microenvironment. The present review delineates the current research trends in this sphere, focusing on material perspective and the target tissues (e.g., neural, liver, pancreatic, renal, and intestinal). The review winds up with a discussion on the current limitations and future perspective to provide a basis for future research.

印度理工学院Tapas K. Maiti等 | 用于类器官培养的化学成分确定且性质可调的水凝胶平台的最新进展

本综述论文聚焦具有可调特性和明确化学成分的水凝胶,并讨论其在类器官构建中的应用。类器官培养技术的最新发展使得在体外条件下密切概括不同组织的内在特征成为可能。这些类器官可以充当传统2D / 3D培养模型和体内模型之间的转化桥梁,并用于研究组织发育过程、疾病模型构建和药物筛选。基质胶(Matrigel)和组织特异性细胞外基质已被证明可以有效地支持类器官发育;然而,它们在化学成分上没有明确的界定,且性质不可调的特性以及相关的批次间的变化通常会限制实验过程的可重复性。在这方面,化学成分明确的平台为优化和重建组织特异性微环境提供了更广泛的机会。本综述描述了该领域目前的研究趋势,侧重于材料视角和靶组织(例如神经、肝脏、胰腺、肾脏和肠道)。本文还讨论了当前的局限性和未来前景,为未来的研究提供了基础。

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