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Received: 2001-12-28
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Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits
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HUANG Xian-mei, ZHU Wei-hua, KANG Man-li. Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits[J]. Journal of Zhejiang University Science A, 2003, 4(1): 114-120.
@article{title="Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits",
author="HUANG Xian-mei, ZHU Wei-hua, KANG Man-li",
journal="Journal of Zhejiang University Science A",
volume="4",
number="1",
pages="114-120",
year="2003",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2003.0114"
}
%0 Journal Article
%T Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits
%A HUANG Xian-mei
%A ZHU Wei-hua
%A KANG Man-li
%J Journal of Zhejiang University SCIENCE A
%V 4
%N 1
%P 114-120
%@ 1869-1951
%D 2003
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2003.0114
TY - JOUR
T1 - Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits
A1 - HUANG Xian-mei
A1 - ZHU Wei-hua
A1 - KANG Man-li
J0 - Journal of Zhejiang University Science A
VL - 4
IS - 1
SP - 114
EP - 120
%@ 1869-1951
Y1 - 2003
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2003.0114
Abstract: To investigate the effect of doxorubicin (DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR) Ca2+ transport proteins and the mechanism of taurine (Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX, 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function, concentration of calcium in cardiomyocytes (Myo[Ca2+]i), activity of SR ca2+-ATPase(SERCA2a), level of SERCA2a mRNA and Ca2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR ca2+-ATPase and level of SERCA2a mRNA decreased, while Myo[Ca2+]i increased in DOX-treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca2+]i and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX-induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.
[1]Applequist, S.E., Keyna, U. Calvin, M.R., Beck-Engeser,G.B., Raman, C. and Tack, H.M., 1995. Sequence of the rabbit glyceraldehyde-3-phosphate dehydrogenase-encoding cDNA. Gene, 163(2):325-326.
[2]Beuckelmann,D.J., Nabauer,M. and Erdmann,E.,1992. Intracellular calcium handling in isolated ventricular myocytes from patients with terminal heart failure. Circulation, 85(3):1046-1055.
[3]Bradford, M.M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem, 72:248-254.
[4]Huang, X.M. and Kang, M.L., 1999. The gene expression and regulation of the myocardial sarcoplasmic reticulum Ca2+ transport proteins. Foreign Medical Sciences, Section of Pediatrics, 26(6):284-286 (in Chinese).
[5]Huxtable, R.J. 1992. Physiological actions of taurine. Physiological Reviews,72(1):101-144.
[6]Ji,Y, Wu, L.L. and Fu,F.Y., 1997. Fengying. Effects of taurine on Ca2+ transport of rat cardiac sarcoplasmic reticulum. J. Beijing Medical University,29(1):29-31(in Chinese).
[7]Jones,L.R., Besch, H.R. and Fleming, J.W., 1979, McConnaughey, M.M; Watanabe,A.M. Separation of cardiac sarcolemma from vesicles of cardiac sarcoplasmic reticulum-comparative biochemical analysis of component activities. J Biol Chem, 254(2):530-539.
[8]Kittleson, M.D., Keene, B., Pion, P.D. and Loyer, C.G., 1997. Results of the multicenter spaniel trial(MUST):taurine-and carnitine-responsive dilated cardiomyopathy in American cocker spaniels with decreased plasma taurine concentration. J Vet Intern Med,11(4):204-211.
[9]Labudova, O., Yeghiazarjan, C., Hoger, H. and Lubec, G.,1999. Taurine modulates expression of transporters in rat brain and heart. Amino Acids,17(3):301-313.
[10]MacLennan, D.H., Brandl, C.J., Korczak, B. and Green, N.M., 1985, Amino-acid sequence of a Ca2++Mg2+-dependent ATPase from rabbit muscle sarcoplasmic reticulum,deduced from its complementary DNA sequence. Nature,316(22):696-670.
[11]Maeda, A., Honda,M., Kuramochi,T. and Takabatake, T., 1998. Doxorubicin cardiotoxicity: diastolic cardiac myocyte dysfunction as a result of impaired calcium handling in isolated cardiac myocytes. Jpn Circ J,62(7):505-511.
[12]Otsu,K., Willard, H.F., Khanna, V.K., Zorzato, F., Green, N.M. and MacLennan, D.H.,1990. Molecular cloning of cDNA encoding the Ca2+ release channel(ryanodine receptor) of rabbit cardiac muscle sarcoplasmic reticulum. J Biol Chem,265(23):13472-13483.
[13]Shan, K., Lincoff, M. and Young, J.B., 1996. Anthracycline-induced cardiotoxicity. Ann Intern Med,125(1): 47-58.
[14]Sun, X.W., Ye, Y.X. and Deng, X.X., 1996. Changes in calcium uptake and Ca(2+)-ATPase activity of cardiac sarcoplasmic reticulum in rat associated with hypoxia of various degrees. ACTA Academiae Medicinae Scinicae, 18(1):38-42(in Chinese).
[15]Shu, Y.G., Yang, Y.Z. and Chen, H.Z., 1997. Effects of taurine on calcium in cardiomyocyte. Advance of Physiology, 28(2):157-159 (in Chinese).
[16]Timbrel, J.A., 1995. The in vivo and in vitro protective properties of taurine. Gen Pharmacol, 26:435-446.
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